Gene description for TCIRG1
Gene name T-cell, immune regulator 1, ATPase, H+ transporting, lysosomal V0 subunit A3
Gene symbol TCIRG1
Other names/aliases ATP6N1C
ATP6V0A3
Atp6i
OC-116kDa
OC116
OPTB1
Stv1
TIRC7
Vph1
a3
Species Homo sapiens
 Database cross references - TCIRG1
ExoCarta ExoCarta_10312
Vesiclepedia VP_10312
Entrez Gene 10312
HGNC 11647
MIM 604592
UniProt Q13488  
 TCIRG1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for TCIRG1
Molecular Function
    protein binding GO:0005515 IPI
    proton-transporting ATPase activity, rotational mechanism GO:0046961 IEA
    ATPase binding GO:0051117 IBA
Biological Process
    autophagosome assembly GO:0000045 IEA
    ossification GO:0001503 IEA
    osteoclast proliferation GO:0002158 IEA
    intracellular calcium ion homeostasis GO:0006874 IEA
    apoptotic process GO:0006915 IEA
    inflammatory response GO:0006954 IEA
    cellular defense response GO:0006968 TAS
    vacuolar acidification GO:0007035 IBA
    vacuolar acidification GO:0007035 NAS
    protein catabolic process in the vacuole GO:0007039 ISS
    lysosomal lumen acidification GO:0007042 NAS
    positive regulation of cell population proliferation GO:0008284 TAS
    regulation of proton transport GO:0010155 IEA
    response to silver ion GO:0010272 IEA
    gene expression GO:0010467 IEA
    regulation of gene expression GO:0010468 IEA
    immunoglobulin mediated immune response GO:0016064 IEA
    macroautophagy GO:0016236 ISS
    optic nerve development GO:0021554 IEA
    establishment of cell polarity GO:0030010 IEA
    B cell differentiation GO:0030183 IEA
    T cell differentiation GO:0030217 IEA
    osteoclast differentiation GO:0030316 IEA
    ruffle organization GO:0031529 IEA
    protein localization to organelle GO:0033365 IEA
    memory T cell activation GO:0035709 IEA
    T-helper 1 cell activation GO:0035711 IEA
    T cell homeostasis GO:0043029 IEA
    tooth eruption GO:0044691 IEA
    bone resorption GO:0045453 IEA
    regulation of osteoblast differentiation GO:0045667 IEA
    pH reduction GO:0045851 IEA
    regulation of insulin secretion GO:0050796 IEA
    establishment of vesicle localization GO:0051650 IEA
    retina development in camera-type eye GO:0060041 IEA
    hematopoietic stem cell homeostasis GO:0061484 IEA
    enamel mineralization GO:0070166 IEA
    cellular response to cytokine stimulus GO:0071345 IEA
    phagosome acidification GO:0090383 IEA
    dentin mineralization GO:0097188 IEA
    proton transmembrane transport GO:1902600 NAS
Subcellular Localization
    vacuolar proton-transporting V-type ATPase, V0 domain GO:0000220 IEA
    nucleus GO:0005634 IEA
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 NAS
    lysosomal membrane GO:0005765 TAS
    late endosome GO:0005770 IEA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 NAS
    plasma membrane GO:0005886 TAS
    endosome membrane GO:0010008 NAS
    endosome membrane GO:0010008 TAS
    apical plasma membrane GO:0016324 IDA
    vacuolar proton-transporting V-type ATPase complex GO:0016471 IBA
    phagocytic vesicle membrane GO:0030670 TAS
    proton-transporting V-type ATPase complex GO:0033176 NAS
    ficolin-1-rich granule membrane GO:0101003 TAS
 Experiment description of studies that identified TCIRG1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for TCIRG1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CCDC115 84317
Affinity Capture-MS Homo sapiens
2 ATP6V0D1 9114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 ATP6V1B2 526
Affinity Capture-MS Homo sapiens
4 ABCC2 1244
Co-fractionation Homo sapiens
5 ADCY3 109
Affinity Capture-MS Homo sapiens
6 CSNK1G3 1456
Affinity Capture-MS Homo sapiens
7 PPP1R15B  
Affinity Capture-MS Homo sapiens
8 TLX3  
Two-hybrid Homo sapiens
9 SARAF  
Affinity Capture-MS Homo sapiens
10 ZDHHC9  
Affinity Capture-MS Homo sapiens
11 ATP6V0D2 245972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 ATP6AP2 10159
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 KCNT2  
Affinity Capture-MS Homo sapiens
14 VAPA 9218
Co-fractionation Homo sapiens
15 ABCB9  
Affinity Capture-MS Homo sapiens
16 TYW1B  
Affinity Capture-MS Homo sapiens
17 NDFIP1 80762
Affinity Capture-MS Homo sapiens
18 ATP6V1C1 528
Affinity Capture-MS Homo sapiens
19 POGZ 23126
Two-hybrid Homo sapiens
20 KCNK1  
Two-hybrid Homo sapiens
21 ICA1  
Affinity Capture-MS Homo sapiens
22 GOLT1B 51026
Affinity Capture-MS Homo sapiens
23 ATP6V1D 51382
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
24 TMEM199  
Affinity Capture-MS Homo sapiens
25 ERLEC1 27248
Co-fractionation Homo sapiens
26 ATG9A 79065
Affinity Capture-MS Homo sapiens
27 RPS15 6209
Co-fractionation Homo sapiens
28 EGFR 1956
Affinity Capture-MS Homo sapiens
29 PDE3B  
Affinity Capture-MS Homo sapiens
30 ATP6V1G2  
Affinity Capture-MS Homo sapiens
31 PGAM2 5224
Affinity Capture-MS Homo sapiens
32 ATP6V1F 9296
Affinity Capture-MS Homo sapiens
33 BAG6 7917
Affinity Capture-MS Homo sapiens
34 GOLGA7 51125
Affinity Capture-MS Homo sapiens
35 RPS18 6222
Co-fractionation Homo sapiens
36 ATP6V0C 527
Affinity Capture-MS Homo sapiens
37 TERF1 7013
Two-hybrid Homo sapiens
38 ADD2 119
Affinity Capture-MS Homo sapiens
39 DNAJC18  
Affinity Capture-MS Homo sapiens
40 KIAA2013 90231
Affinity Capture-MS Homo sapiens
41 ATP6AP1 537
Affinity Capture-MS Homo sapiens
42 ATL2 64225
Affinity Capture-MS Homo sapiens
43 AP3S2 10239
Affinity Capture-MS Homo sapiens
44 ATP6V1E1 529
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
45 SORT1 6272
Affinity Capture-MS Homo sapiens
46 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
47 FLOT1 10211
Affinity Capture-MS Homo sapiens
48 DCAF10  
Affinity Capture-MS Homo sapiens
49 ATP6V0A2 23545
Affinity Capture-MS Homo sapiens
50 TTC9C 283237
Affinity Capture-MS Homo sapiens
51 ATP6V1B1 525
Co-fractionation Homo sapiens
52 TMX2 51075
Affinity Capture-MS Homo sapiens
53 VMA21  
Affinity Capture-MS Homo sapiens
54 ATP6V0A1 535
Affinity Capture-MS Homo sapiens
55 EWSR1 2130
Co-fractionation Homo sapiens
56 AP3D1 8943
Affinity Capture-MS Homo sapiens
57 PLD6  
Affinity Capture-MS Homo sapiens
58 FLOT2 2319
Affinity Capture-MS Homo sapiens
59 ATP2A3 489
Affinity Capture-MS Homo sapiens
60 TMEM63A 9725
Affinity Capture-MS Homo sapiens
61 DDX28  
Co-fractionation Homo sapiens
62 TAP2 6891
Affinity Capture-MS Homo sapiens
63 SLC30A5 64924
Co-fractionation Homo sapiens
View the network image/svg+xml



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