|
 Gene description for RNF123 |
| Gene name |
ring finger protein 123 |
| Gene symbol |
RNF123 |
| Other names/aliases |
FP1477
KPC1 |
| Species |
Homo sapiens |
| Database cross references - RNF123 |
| ExoCarta |
ExoCarta_63891 |
| Entrez Gene |
63891 |
| HGNC |
21148 |
| MIM |
614472 |
| UniProt |
Q5XPI4
|
| RNF123 identified in exosomes derived from the following tissue/cell type |
|
Hepatocellular carcinoma cells
|
26054723
|
|
Mesenchymal stem cells
|
Unpublished / Not applicable
|
| Gene ontology annotations for RNF123 |
|
|
| Experiment description of studies that identified RNF123 in exosomes |
| 1 |
| Experiment ID |
235 |
| ISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
TSG101|Alix|HSC70|GAPDH
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✔
HSP90B1
|
EV Negative markers |
|
✔
qNano
|
EV Particle analysis
|
|
| Identified molecule |
mRNA
|
| Identification method |
RNA Sequencing
|
| PubMed ID |
26054723
|
| Organism |
Homo sapiens |
| Experiment description |
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs |
| Authors |
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N |
| Journal name |
Carcinogenesis
|
| Publication year |
2015 |
| Sample |
Hepatocellular carcinoma cells |
| Sample name |
HKCI-8 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient |
| Flotation density |
1.13-1.19 g/mL
|
| Molecules identified in the study |
Protein
RNA |
| Methods used in the study |
Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing |
|
|
| 2 |
| Experiment ID |
126 |
| ISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
GAPDH
|
EV Cytosolic markers |
|
✘
|
EV Membrane markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
| PubMed ID |
Unpublished / Not applicable
|
| Organism |
Homo sapiens |
| Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
| Authors |
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim |
| Journal name |
Mesenchymal Stem Cell Therapy
|
| Publication year |
2011 |
| Sample |
Mesenchymal stem cells |
| Sample name |
huES9.E1 |
| Isolation/purification methods |
HPLC |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Antobody array
Mass spectrometry |
|
|
| Protein-protein interactions for RNF123 |
|
|
| Pathways in which RNF123 is involved |
|
|
|
