Gene description for EFEMP1
Gene name EGF containing fibulin-like extracellular matrix protein 1
Gene symbol EFEMP1
Other names/aliases DHRD
DRAD
FBLN3
FBNL
FIBL-3
MLVT
MTLV
S1-5
Species Homo sapiens
 Database cross references - EFEMP1
ExoCarta ExoCarta_2202
Entrez Gene 2202
HGNC 3218
MIM 601548
UniProt Q12805  
 EFEMP1 identified in exosomes derived from the following tissue/cell type
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Nasopharyngeal carcinoma cells 25857718    
Urine 19056867    
 Gene ontology annotations for EFEMP1
Molecular Function
    protein binding GO:0005515 IPI
    epidermal growth factor-activated receptor activity GO:0005006 IDA
    calcium ion binding GO:0005509 IEA
    epidermal growth factor receptor binding GO:0005154 IDA
    growth factor activity GO:0008083 IEA
Biological Process
    epidermal growth factor receptor signaling pathway GO:0007173 IDA
    extracellular matrix organization GO:0030198 TAS
    visual perception GO:0007601 TAS
    regulation of transcription, DNA-templated GO:0006355 IDA
    negative regulation of chondrocyte differentiation GO:0032331 IDA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
Subcellular Localization
    extracellular matrix GO:0031012 IDA
    proteinaceous extracellular matrix GO:0005578 IEA
    extracellular exosome GO:0070062 IDA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 IDA
 Experiment description of studies that identified EFEMP1 in exosomes
1
Experiment ID 219
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name C666-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 220
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP69
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 221
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25857718    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from nasopharyngeal carcinoma cell identifies intercellular transfer of angiogenic proteins
Authors Chan YK, Zhang H, Liu P, Tsao GS, Li Lung M, Mak NK, Ngok-Shun Wong R, Ying-Kit Yue P
Journal name Int J Cancer
Publication year 2015
Sample Nasopharyngeal carcinoma cells
Sample name NP460
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.17-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for EFEMP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SGTA 6449
Two-hybrid Homo sapiens
2 SMAD4  
Affinity Capture-MS Homo sapiens
3 ARAF 369
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which EFEMP1 is involved
PathwayEvidenceSource
Molecules associated with elastic fibres TAS Reactome





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