Gene description for WDFY3
Gene name WD repeat and FYVE domain containing 3
Gene symbol WDFY3
Other names/aliases ALFY
ZFYVE25
Species Homo sapiens
 Database cross references - WDFY3
ExoCarta ExoCarta_23001
Vesiclepedia VP_23001
Entrez Gene 23001
HGNC 20751
UniProt Q8IZQ1  
 WDFY3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for WDFY3
Molecular Function
    protein binding GO:0005515 IPI
    1-phosphatidylinositol binding GO:0005545 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    aggrephagy GO:0035973 IBA
    aggrephagy GO:0035973 IMP
Subcellular Localization
    autophagosome membrane GO:0000421 IDA
    nuclear envelope GO:0005635 IDA
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    autophagosome GO:0005776 IMP
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    membrane GO:0016020 IDA
    inclusion body GO:0016234 IDA
    PML body GO:0016605 IDA
    axon GO:0030424 IEA
    nuclear membrane GO:0031965 IEA
    Atg12-Atg5-Atg16 complex GO:0034274 IDA
    perikaryon GO:0043204 IEA
 Experiment description of studies that identified WDFY3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for WDFY3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GABARAPL1 23710
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 MAP1LC3C  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
3 ATG5 9474
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 NFE2L1  
Affinity Capture-MS Homo sapiens
5 ATG9A 79065
Proximity Label-MS Homo sapiens
6 HLA-E 3133
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 HCST  
Affinity Capture-MS Homo sapiens
8 PDCD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 MDFI  
Two-hybrid Homo sapiens
10 FPR1  
Affinity Capture-MS Homo sapiens
11 TERF2IP 54386
Affinity Capture-MS Homo sapiens
12 FFAR1  
Affinity Capture-MS Homo sapiens
13 S100A4 6275
Affinity Capture-MS Homo sapiens
14 APAF1 317
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 HLA-B 3106
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SUV39H1  
Two-hybrid Homo sapiens
17 KLRC1  
Affinity Capture-MS Homo sapiens
18 TACSTD2 4070
Affinity Capture-MS Homo sapiens
19 ITFG3 83986
Affinity Capture-MS Homo sapiens
20 VCAM1 7412
Affinity Capture-MS Homo sapiens
21 GPR17 2840
Affinity Capture-MS Homo sapiens
22 Mad2l1bp  
Affinity Capture-MS Mus musculus
23 BSG 682
Affinity Capture-MS Homo sapiens
24 Lrr1  
Affinity Capture-MS Mus musculus
25 DPYSL4 10570
Affinity Capture-MS Homo sapiens
26 GCGR  
Affinity Capture-MS Homo sapiens
27 BAG6 7917
Affinity Capture-MS Homo sapiens
28 GABARAP 11337
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Co-localization Homo sapiens
29 GPR182  
Affinity Capture-MS Homo sapiens
30 TRIM66  
Affinity Capture-MS Homo sapiens
31 LCN1 3933
Affinity Capture-MS Homo sapiens
32 LZTR1 8216
Affinity Capture-MS Homo sapiens
33 CEP76  
Two-hybrid Homo sapiens
34 DEF8  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RHOT2 89941
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 DAXX  
Affinity Capture-MS Homo sapiens
37 RTEL1 51750
Affinity Capture-MS Homo sapiens
38 ZBTB44  
Two-hybrid Homo sapiens
39 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
40 ATG16L1 55054
Affinity Capture-Western Homo sapiens
41 HERC1 8925
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 FURIN 5045
Affinity Capture-MS Homo sapiens
43 ICAM1 3383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 CD79B 974
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 LRRC25  
Affinity Capture-MS Homo sapiens
46 PTH2R  
Affinity Capture-MS Homo sapiens
47 ATG12  
Affinity Capture-Western Homo sapiens
48 MGARP  
Affinity Capture-MS Homo sapiens
49 TRAF6 7189
Affinity Capture-Western Homo sapiens
50 GPR12  
Affinity Capture-MS Homo sapiens
51 NR3C1 2908
Proximity Label-MS Homo sapiens
52 AQP3  
Affinity Capture-MS Homo sapiens
53 OPRL1 4987
Affinity Capture-MS Homo sapiens
54 WEE1 7465
Affinity Capture-MS Homo sapiens
55 NXF1 10482
Affinity Capture-RNA Homo sapiens
56 SGTB  
Affinity Capture-MS Homo sapiens
57 PRMT6  
Two-hybrid Homo sapiens
58 LACRT 90070
Affinity Capture-MS Homo sapiens
59 TMEM132A 54972
Affinity Capture-MS Homo sapiens
60 WDR60 55112
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 GPR45  
Affinity Capture-MS Homo sapiens
62 WDR75  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 P2RY10  
Affinity Capture-MS Homo sapiens
64 Cxxc1  
Affinity Capture-MS Mus musculus
65 TRIM39  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 RAB5C 5878
Affinity Capture-MS Homo sapiens
67 EEF2K  
Affinity Capture-MS Homo sapiens
68 MAGEA8  
Affinity Capture-MS Homo sapiens
69 SLC2A12 154091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 ALG13 79868
Affinity Capture-MS Homo sapiens
71 SCGB2A1 4246
Affinity Capture-MS Homo sapiens
72 OPALIN  
Affinity Capture-MS Homo sapiens
73 EDEM1  
Affinity Capture-MS Homo sapiens
74 GABARAPL2 11345
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
75 SQSTM1 8878
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
76 DMWD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 SHMT2 6472
Affinity Capture-RNA Homo sapiens
78 FLCN 201163
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
79 DCAF6 55827
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 DDX11 1663
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 MAP1LC3A 84557
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
82 MOV10 4343
Affinity Capture-RNA Homo sapiens
83 NEDD1 121441
Affinity Capture-MS Homo sapiens
84 RAB11A 8766
Proximity Label-MS Homo sapiens
85 P2RX2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 PRMT1 3276
Two-hybrid Homo sapiens
87 Tecpr2  
Affinity Capture-MS Mus musculus
View the network image/svg+xml
 Pathways in which WDFY3 is involved
No pathways found





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