Gene description for MKNK2
Gene name MAP kinase interacting serine/threonine kinase 2
Gene symbol MKNK2
Other names/aliases GPRK7
MNK2
Species Homo sapiens
 Database cross references - MKNK2
ExoCarta ExoCarta_2872
Entrez Gene 2872
HGNC 7111
MIM 605069
UniProt Q9HBH9  
 MKNK2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for MKNK2
Molecular Function
    ATP binding GO:0005524 IDA
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 IDA
    metal ion binding GO:0046872 IEA
Biological Process
    cellular response to arsenic-containing substance GO:0071243 IDA
    cell surface receptor signaling pathway GO:0007166 TAS
    protein phosphorylation GO:0006468 IDA
    hemopoiesis GO:0030097 IDA
    regulation of translation GO:0006417 IEA
    extrinsic apoptotic signaling pathway in absence of ligand GO:0097192 IEA
    intracellular signal transduction GO:0035556 IDA
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    nucleolus GO:0005730 IDA
    PML body GO:0016605 IEA
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified MKNK2 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for MKNK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK1 5594
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
2 ESR1  
Two-hybrid Homo sapiens
3 ESR2  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
4 EIF4G1 1981
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
5 MKNK2 2872
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MKNK2 is involved
No pathways found





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