Gene description for MKNK2
Gene name
MAP kinase interacting serine/threonine kinase 2
Gene symbol
MKNK2
Other names/aliases
GPRK7 MNK2
Species
Homo sapiens
Database cross references - MKNK2
ExoCarta
ExoCarta_2872
Entrez Gene
2872
HGNC
7111
MIM
605069
UniProt
Q9HBH9
MKNK2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells
26054723
Hepatocytes
26054723
Gene ontology annotations for MKNK2
Experiment description of studies that identified MKNK2 in exosomes
1
Experiment ID
235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
2
Experiment ID
237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘
EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
Protein-protein interactions for MKNK2
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
MAPK1
5594
Two-hybrid
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
Two-hybrid
Homo sapiens
2
ESR1
Two-hybrid
Homo sapiens
3
ESR2
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
4
EIF4G1
1981
Affinity Capture-Western
Homo sapiens
Biochemical Activity
Homo sapiens
5
MKNK2
2872
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
View the network
image/svg+xml
Pathways in which MKNK2 is involved
No pathways found