Gene description for KRT82
Gene name keratin 82, type II
Gene symbol KRT82
Other names/aliases HB2
Hb-2
KRTHB2
Species Homo sapiens
 Database cross references - KRT82
ExoCarta ExoCarta_3888
Vesiclepedia VP_3888
Entrez Gene 3888
HGNC 6459
MIM 601078
UniProt Q9NSB4  
 KRT82 identified in exosomes derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for KRT82
Molecular Function
    protein binding GO:0005515 IPI
    structural constituent of skin epidermis GO:0030280 IBA
    structural constituent of skin epidermis GO:0030280 NAS
Biological Process
    keratinization GO:0031424 IBA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    keratin filament GO:0045095 IBA
    keratin filament GO:0045095 IDA
    keratin filament GO:0045095 NAS
 Experiment description of studies that identified KRT82 in exosomes
1
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for KRT82
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GAN 8139
Affinity Capture-MS Homo sapiens
2 RNF40 9810
Affinity Capture-MS Homo sapiens
3 TWF2 11344
Affinity Capture-MS Homo sapiens
4 GRB2 2885
Affinity Capture-MS Homo sapiens
5 KRT33B 3884
Two-hybrid Homo sapiens
6 DLG3 1741
Affinity Capture-MS Homo sapiens
7 RC3H2  
Affinity Capture-MS Homo sapiens
8 VAC14 55697
Two-hybrid Homo sapiens
9 KRT35 3886
Two-hybrid Homo sapiens
10 KRT34  
Two-hybrid Homo sapiens
11 PLSCR4  
Two-hybrid Homo sapiens
12 EPPK1 83481
Affinity Capture-MS Homo sapiens
13 EPS15 2060
Affinity Capture-MS Homo sapiens
14 PLEKHB2 55041
Affinity Capture-MS Homo sapiens
15 HOXA1 3198
Two-hybrid Homo sapiens
16 KRT27 342574
Two-hybrid Homo sapiens
17 ELF3 1999
Affinity Capture-MS Homo sapiens
18 KRT15 3866
Two-hybrid Homo sapiens
19 CCDC101  
Affinity Capture-MS Homo sapiens
20 EFNA4  
Affinity Capture-MS Homo sapiens
21 DNPEP 23549
Affinity Capture-MS Homo sapiens
22 HGS 9146
Two-hybrid Homo sapiens
23 KRT40  
Two-hybrid Homo sapiens
24 COL8A1 1295
Two-hybrid Homo sapiens
25 WTAP 9589
Affinity Capture-MS Homo sapiens
26 LYAR 55646
Affinity Capture-MS Homo sapiens
27 OBSL1 23363
Affinity Capture-MS Homo sapiens
28 KRT16 3868
Co-fractionation Homo sapiens
View the network image/svg+xml
 Pathways in which KRT82 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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