ExoCarta: Gene summary

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Gene description for OASL
Gene name	2'-5'-oligoadenylate synthetase-like
Gene symbol	OASL
Other names/aliases	OASLd TRIP-14 TRIP14 p59 OASL p59-OASL p59OASL
Species	Homo sapiens
Database cross references - OASL
ExoCarta	ExoCarta_8638
Vesiclepedia	VP_8638
Entrez Gene	8638
HGNC	8090
MIM	603281
UniProt	Q15646
OASL identified in exosomes derived from the following tissue/cell type
Gastric cancer cells	33991177
Gastric cancer cells	33991177
Gastric cancer cells	33991177

Gene ontology annotations for OASL

Molecular Function
	2'-5'-oligoadenylate synthetase activity	GO:0001730	IDA
	DNA binding	GO:0003677	IDA
	RNA binding	GO:0003723	HDA
	double-stranded RNA binding	GO:0003725	IBA
	double-stranded RNA binding	GO:0003725	IDA
	protein binding	GO:0005515	IPI
	nuclear thyroid hormone receptor binding	GO:0046966	TAS
Biological Process
	response to virus	GO:0009615	IDA
	negative regulation of viral genome replication	GO:0045071	IBA
	negative regulation of viral genome replication	GO:0045071	IDA
	defense response to virus	GO:0051607	IDA
	type I interferon-mediated signaling pathway	GO:0060337	IBA
	interleukin-27-mediated signaling pathway	GO:0070106	IBA
	interleukin-27-mediated signaling pathway	GO:0070106	IEP
	antiviral innate immune response	GO:0140374	IBA
	positive regulation of RIG-I signaling pathway	GO:1900246	IDA
Subcellular Localization
	nucleoplasm	GO:0005654	IBA
	nucleoplasm	GO:0005654	IDA
	nucleolus	GO:0005730	IDA
	cytoplasm	GO:0005737	IDA
	cytosol	GO:0005829	IBA
	cytosol	GO:0005829	IDA
	cytosol	GO:0005829	TAS
	membrane	GO:0016020	HDA
	membrane	GO:0016020	IBA

Experiment description of studies that identified OASL in exosomes

Experiment ID

363

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

364

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

365

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Protein-protein interactions for OASL

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

GNL2

29889

Affinity Capture-MS

Homo sapiens

RPSA

3921

Affinity Capture-MS

Homo sapiens

ZNF668

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPL26L1

51121

Affinity Capture-MS

Homo sapiens

ZNF771

Affinity Capture-MS

Homo sapiens

ZBTB11

Affinity Capture-MS

Homo sapiens

ZNF346

Affinity Capture-MS

Homo sapiens

KIAA0020

9933

Affinity Capture-MS

Homo sapiens

NOP9

161424

Affinity Capture-MS

Homo sapiens

NOP14

8602

Affinity Capture-MS

Homo sapiens

CENPC

Affinity Capture-MS

Homo sapiens

LARP1B

55132

Affinity Capture-MS

Homo sapiens

RPS19

6223

Affinity Capture-MS

Homo sapiens

RPL14

9045

Affinity Capture-MS

Homo sapiens

LOC100132735

Protein-RNA

Homo sapiens

RSBN1

Affinity Capture-MS

Homo sapiens

NUFIP1

Affinity Capture-MS

Homo sapiens

UTP3

57050

Affinity Capture-MS

Homo sapiens

SOX2

Affinity Capture-MS

Homo sapiens

Spire2

Affinity Capture-MS

Mus musculus

ZNF189

Affinity Capture-MS

Homo sapiens

WHSC1

7468

Affinity Capture-MS

Homo sapiens

LLPH

Affinity Capture-MS

Homo sapiens

DDX24

57062

Affinity Capture-MS

Homo sapiens

RRP12

23223

Affinity Capture-MS

Homo sapiens

TM9SF4

9777

Affinity Capture-MS

Homo sapiens

NPM1

4869

Affinity Capture-MS

Homo sapiens

KRI1

Affinity Capture-MS

Homo sapiens

HELZ2

Affinity Capture-MS

Homo sapiens

RPF2

84154

Affinity Capture-MS

Homo sapiens

ZCCHC3

Affinity Capture-MS

Homo sapiens

STAU2

27067

Affinity Capture-MS

Homo sapiens

PRKRA

8575

Affinity Capture-MS

Homo sapiens

PATZ1

Affinity Capture-MS

Homo sapiens

ABT1

29777

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RRP8

Affinity Capture-MS

Homo sapiens

CD14

929

Affinity Capture-MS

Homo sapiens

RPS3A

6189

Affinity Capture-MS

Homo sapiens

Klc3

Affinity Capture-MS

Mus musculus

C18orf21

Affinity Capture-MS

Homo sapiens

RPL19

6143

Affinity Capture-MS

Homo sapiens

RBM19

9904

Affinity Capture-MS

Homo sapiens

CCDC86

Affinity Capture-MS

Homo sapiens

TMEM63B

55362

Affinity Capture-MS

Homo sapiens

ZCRB1

Affinity Capture-MS

Homo sapiens

BRIX1

55299

Affinity Capture-MS

Homo sapiens

SEZ6L2

26470

Two-hybrid

Homo sapiens

ZKSCAN8

Affinity Capture-MS

Homo sapiens

SCAF1

Affinity Capture-MS

Homo sapiens

DDX21

9188

Affinity Capture-MS

Homo sapiens

RPL4

6124

Affinity Capture-MS

Homo sapiens

RBM28

55131

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RPS10

6204

Affinity Capture-MS

Homo sapiens

ZNF616

Affinity Capture-MS

Homo sapiens

STAU1

6780

Affinity Capture-MS

Homo sapiens

NEIL1

Affinity Capture-MS

Homo sapiens

SRSF6

6431

Affinity Capture-MS

Homo sapiens

PURG

Affinity Capture-MS

Homo sapiens

HIST1H2AM

8336

Affinity Capture-MS

Homo sapiens

NKRF

55922

Affinity Capture-MS

Homo sapiens

NOC4L

79050

Affinity Capture-MS

Homo sapiens

HIST1H1E

3008

Affinity Capture-MS

Homo sapiens

REXO4

Affinity Capture-MS

Homo sapiens

DDX54

79039

Affinity Capture-MS

Homo sapiens

LYAR

55646

Affinity Capture-MS

Homo sapiens

Map9

Affinity Capture-MS

Mus musculus

RPLP0

6175

Affinity Capture-MS

Homo sapiens

PIK3C2A

5286

Affinity Capture-MS

Homo sapiens

MAGEB2

4113

Affinity Capture-MS

Homo sapiens

SPTY2D1

Affinity Capture-MS

Homo sapiens

DDX27

55661

Affinity Capture-MS

Homo sapiens

GLTSCR2

Affinity Capture-MS

Homo sapiens

LARP1

23367

Affinity Capture-MS

Homo sapiens

SF3B2

10992

Affinity Capture-MS

Homo sapiens

ZNF316

Affinity Capture-MS

Homo sapiens

SPATS2L

26010

Affinity Capture-MS

Homo sapiens

KMT2A

Affinity Capture-MS

Homo sapiens

MUS81

Affinity Capture-MS

Homo sapiens

RPS2

6187

Affinity Capture-MS

Homo sapiens

ECD

Affinity Capture-MS

Homo sapiens

POP1

10940

Affinity Capture-MS

Homo sapiens

ZC3HAV1

56829

Affinity Capture-MS

Homo sapiens

ZBTB24

Affinity Capture-MS

Homo sapiens

SIRT7

Affinity Capture-MS

Homo sapiens

RPL35

11224

Affinity Capture-MS

Homo sapiens

TAF1B

Affinity Capture-MS

Homo sapiens

ZNF354A

Affinity Capture-MS

Homo sapiens

RPS6

6194

Affinity Capture-MS

Homo sapiens

RPL3

6122

Affinity Capture-MS

Homo sapiens

DHX30

22907

Affinity Capture-MS

Homo sapiens

DHX57

90957

Affinity Capture-MS

Homo sapiens

ZNF777

Affinity Capture-MS

Homo sapiens

PRKRIR

Affinity Capture-MS

Homo sapiens

CEBPZ

Affinity Capture-MS

Homo sapiens

ZCCHC6

79670

Affinity Capture-MS

Homo sapiens

GZF1

Affinity Capture-MS

Homo sapiens

HP1BP3

50809

Affinity Capture-MS

Homo sapiens

HIST1H1A

3024

Affinity Capture-MS

Homo sapiens

FYTTD1

Affinity Capture-MS

Homo sapiens

100

NOP16

51491

Affinity Capture-MS

Homo sapiens

101

ASH1L

55870

Affinity Capture-MS

Homo sapiens

102

FTSJ3

117246

Affinity Capture-MS

Homo sapiens

103

NGDN

Affinity Capture-MS

Homo sapiens

104

ZNF574

Affinity Capture-MS

Homo sapiens

105

RPL13

6137

Affinity Capture-MS

Homo sapiens

106

YBX1

4904

Affinity Capture-MS

Homo sapiens

107

DDX50

79009

Affinity Capture-MS

Homo sapiens

108

ZNF624

57547

Affinity Capture-MS

Homo sapiens

109

PRDM10

Affinity Capture-MS

Homo sapiens

110

ZNF689

Affinity Capture-MS

Homo sapiens

111

PRDM15

Affinity Capture-MS

Homo sapiens

112

ZNF445

Affinity Capture-MS

Homo sapiens

113

DGCR8

Affinity Capture-MS

Homo sapiens

114

NSA2

Affinity Capture-MS

Homo sapiens

115

DDX58

23586

Affinity Capture-RNA

Homo sapiens

116

ZNF317

57693

Affinity Capture-MS

Homo sapiens

117

KRR1

11103

Affinity Capture-MS

Homo sapiens

118

LARP7

51574

Affinity Capture-MS

Homo sapiens

119

ZNF646

Affinity Capture-MS

Homo sapiens

120

TTF1

Affinity Capture-MS

Homo sapiens

121

MOV10

4343

Affinity Capture-MS

Homo sapiens

122

YBX3

8531

Affinity Capture-MS

Homo sapiens

123

YTHDC2

64848

Affinity Capture-MS

Homo sapiens

124

SORT1

6272

Affinity Capture-MS

Homo sapiens

125

CIT

11113

Affinity Capture-MS

Homo sapiens

126

HERC5

51191

Affinity Capture-MS

Homo sapiens

127

ZNF845

Affinity Capture-MS

Homo sapiens

128

CCR1

Affinity Capture-MS

Homo sapiens

129

FARS2

10667

Affinity Capture-MS

Homo sapiens

130

ZNF865

Affinity Capture-MS

Homo sapiens

131

ZSCAN25

Affinity Capture-MS

Homo sapiens

132

IMP4

Affinity Capture-MS

Homo sapiens

View the network

Pathways in which OASL is involved

Pathway	Evidence	Source
Antiviral mechanism by IFN-stimulated genes	TAS	Reactome
Cytokine Signaling in Immune system	TAS	Reactome
Immune System	TAS	Reactome
Interferon alpha/beta signaling	TAS	Reactome
Interferon gamma signaling	TAS	Reactome
Interferon Signaling	TAS	Reactome
OAS antiviral response	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here