Gene description for CALM3
Gene name calmodulin 3 (phosphorylase kinase, delta)
Gene symbol CALM3
Other names/aliases HEL-S-72
PHKD
PHKD3
Species Homo sapiens
 Database cross references - CALM3
ExoCarta ExoCarta_808
Vesiclepedia VP_808
Entrez Gene 808
HGNC 1449
MIM 114183
UniProt P62158  
 CALM3 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 17956143    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
Saliva 19199708    
Squamous carcinoma cells 20124223    
Thymus 23844026    
Tracheobronchial cells 19190083    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for CALM3
Molecular Function
    calcium ion binding GO:0005509 IBA
    calcium ion binding GO:0005509 IDA
    protein binding GO:0005515 IPI
    adenylate cyclase binding GO:0008179 IPI
    adenylate cyclase activator activity GO:0010856 IDA
    protein kinase binding GO:0019901 IPI
    titin binding GO:0031432 IPI
    protein serine/threonine kinase activator activity GO:0043539 IDA
    protein serine/threonine kinase activator activity GO:0043539 TAS
    transmembrane transporter binding GO:0044325 IPI
    calcium-dependent protein binding GO:0048306 IEA
    protein phosphatase activator activity GO:0072542 IDA
Biological Process
    G2/M transition of mitotic cell cycle GO:0000086 IEA
    regulation of heart rate GO:0002027 IMP
    detection of calcium ion GO:0005513 IMP
    G protein-coupled receptor signaling pathway GO:0007186 TAS
    positive regulation of peptidyl-threonine phosphorylation GO:0010800 TAS
    negative regulation of peptidyl-threonine phosphorylation GO:0010801 TAS
    regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum GO:0010880 IDA
    regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion GO:0010881 IC
    substantia nigra development GO:0021762 HEP
    positive regulation of protein autophosphorylation GO:0031954 TAS
    regulation of cytokinesis GO:0032465 IMP
    regulation of calcium-mediated signaling GO:0050848 IEA
    response to calcium ion GO:0051592 IDA
    regulation of cardiac muscle contraction GO:0055117 IMP
    negative regulation of ryanodine-sensitive calcium-release channel activity GO:0060315 ISS
    positive regulation of ryanodine-sensitive calcium-release channel activity GO:0060316 IDA
    positive regulation of protein serine/threonine kinase activity GO:0071902 TAS
    calcineurin-mediated signaling GO:0097720 IDA
    regulation of cardiac muscle cell action potential GO:0098901 IMP
    presynaptic endocytosis GO:0140238 IEA
    negative regulation of high voltage-gated calcium channel activity GO:1901842 IMP
    regulation of cell communication by electrical coupling involved in cardiac conduction GO:1901844 IC
    negative regulation of calcium ion export across plasma membrane GO:1905913 IEA
Subcellular Localization
    spindle pole GO:0000922 IDA
    nucleus GO:0005634 HDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 TAS
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    spindle microtubule GO:0005876 IDA
    plasma membrane GO:0005886 TAS
    voltage-gated potassium channel complex GO:0008076 IEA
    sarcomere GO:0030017 IDA
    vesicle GO:0031982 HDA
    protein-containing complex GO:0032991 IDA
    calcium channel complex GO:0034704 IDA
    myelin sheath GO:0043209 IBA
    calyx of Held GO:0044305 IEA
    sperm midpiece GO:0097225 IEA
    presynaptic cytosol GO:0099523 IEA
    catalytic complex GO:1902494 IDA
 Experiment description of studies that identified CALM3 in sEVs
1
Experiment ID 20
MISEV standards
EM
Biophysical techniques
HSP90|CD63|CD81|LAMP1
Enriched markers
GOLGA2|cytochrome c
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17956143    
Organism Homo sapiens
Experiment description Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors "Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name JPR
Publication year 2007
Sample Colorectal cancer cells
Sample name HT29
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Diafiltration
Flotation density 1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
2
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 282
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 283
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 286
MISEV standards
CEM
Biophysical techniques
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
Negative markers
DLS
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors "Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 363
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 364
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 365
MISEV standards
Biophysical techniques
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
DCLK1
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 234
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
12
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
13
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
14
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
15
Experiment ID 211
MISEV standards
EM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
cytochrome c|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
16
Experiment ID 212
MISEV standards
CEM
Biophysical techniques
TSG101|Alix|EpCAM|TFRC
Enriched markers
Cytochrome C|GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
18
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
19
Experiment ID 66
MISEV standards
IEM
Biophysical techniques
TSG101|Alix|CD63|CD81
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
20
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
22
Experiment ID 34
MISEV standards
EM|IEM
Biophysical techniques
TSG101|CD63|MHCI|MHCII
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19190083    
Organism Homo sapiens
Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors "Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK"
Journal name FASEB
Publication year 2009
Sample Tracheobronchial cells
Sample name Tracheobronchial epithelial cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.16 -1.18 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
FACS
23
Experiment ID 196
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
24
Experiment ID 197
MISEV standards
EM
Biophysical techniques
Alix|TSG101|HSP70|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CALM3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MRPS27 23107
Affinity Capture-MS Homo sapiens
2 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
3 SLIRP 81892
Affinity Capture-MS Homo sapiens
4 C5AR2  
Two-hybrid Homo sapiens
5 PPP3CA 5530
Affinity Capture-MS Homo sapiens
6 KIF23 9493
Affinity Capture-MS Homo sapiens
7 KIF1A 547
Affinity Capture-MS Homo sapiens
8 MYO5B 4645
Affinity Capture-MS Homo sapiens
9 ARPC5L 81873
Affinity Capture-MS Homo sapiens
10 TBC1D4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 CETN3  
Affinity Capture-MS Homo sapiens
12 EIF3K 27335
Affinity Capture-MS Homo sapiens
13 ARPC2 10109
Affinity Capture-MS Homo sapiens
14 ACTR2 10097
Affinity Capture-MS Homo sapiens
15 PSMD11 5717
Affinity Capture-MS Homo sapiens
16 BTF3 689
Affinity Capture-MS Homo sapiens
17 ADRB2  
Affinity Capture-MS Homo sapiens
18 VPS13A 23230
Affinity Capture-MS Homo sapiens
19 RPL10 6134
Affinity Capture-MS Homo sapiens
20 SRGAP2 23380
Affinity Capture-MS Homo sapiens
21 PRPF3  
Affinity Capture-MS Homo sapiens
22 VAMP2 6844
Co-fractionation Homo sapiens
23 GRAMD1A  
Affinity Capture-MS Homo sapiens
24 AIF1L 83543
Affinity Capture-MS Homo sapiens
25 CAMTA2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
26 CFL1 1072
Affinity Capture-MS Homo sapiens
27 CCDC124 115098
Affinity Capture-MS Homo sapiens
28 STX4 6810
Affinity Capture-MS Homo sapiens
29 CLEC7A  
Two-hybrid Homo sapiens
30 CEP97 79598
Affinity Capture-MS Homo sapiens
31 EIF3M 10480
Affinity Capture-MS Homo sapiens
32 TRIM54  
Two-hybrid Homo sapiens
33 LUZP1 7798
Affinity Capture-MS Homo sapiens
34 LRPPRC 10128
Affinity Capture-MS Homo sapiens
35 CAPZB 832
Affinity Capture-MS Homo sapiens
36 RPS20 6224
Affinity Capture-MS Homo sapiens
37 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
38 WDR76  
Affinity Capture-MS Homo sapiens
39 PCNT  
Affinity Capture-MS Homo sapiens
40 TFAM 7019
Affinity Capture-MS Homo sapiens
41 PPP1CB 5500
Affinity Capture-MS Homo sapiens
42 SPECC1L 23384
Affinity Capture-MS Homo sapiens
43 PCK1 5105
Affinity Capture-MS Homo sapiens
44 MPP5 64398
Affinity Capture-MS Homo sapiens
45 H1FX 8971
Affinity Capture-MS Homo sapiens
46 KCNQ2  
Two-hybrid Homo sapiens
47 PPP3CB  
Affinity Capture-MS Homo sapiens
48 MYLK3 91807
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 FBXW7  
Affinity Capture-MS Homo sapiens
50 RAI14 26064
Affinity Capture-MS Homo sapiens
51 IQGAP1 8826
Affinity Capture-MS Homo sapiens
52 MRPL12 6182
Affinity Capture-MS Homo sapiens
53 ABCD3 5825
Affinity Capture-MS Homo sapiens
54 PSMC6 5706
Affinity Capture-MS Homo sapiens
55 OBSL1 23363
Affinity Capture-MS Homo sapiens
56 RIOK3 8780
Affinity Capture-MS Homo sapiens
57 PHLDB2 90102
Affinity Capture-MS Homo sapiens
58 TOM1 10043
Affinity Capture-MS Homo sapiens
59 C11orf65  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
60 RCAN1  
Affinity Capture-MS Homo sapiens
61 CAMK2B 816
Affinity Capture-MS Homo sapiens
62 MYO1B 4430
Affinity Capture-MS Homo sapiens
63 PEAK1 79834
Affinity Capture-MS Homo sapiens
64 ARHGAP19  
Affinity Capture-MS Homo sapiens
65 ATRX 546
Affinity Capture-MS Homo sapiens
66 PPP3R1 5534
Affinity Capture-MS Homo sapiens
67 SDCBP 6386
Two-hybrid Homo sapiens
68 CTTNBP2NL  
Affinity Capture-MS Homo sapiens
69 CEP131 22994
Affinity Capture-MS Homo sapiens
70 MYL6 4637
Affinity Capture-MS Homo sapiens
71 TBC1D1 23216
Affinity Capture-MS Homo sapiens
72 IQCE 23288
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
73 UNK  
Affinity Capture-RNA Homo sapiens
74 BSG 682
Affinity Capture-MS Homo sapiens
75 UBR4 23352
Affinity Capture-MS Homo sapiens
76 CAMK2A 815
Affinity Capture-MS Homo sapiens
77 KCNN1  
Two-hybrid Homo sapiens
78 HNF1A  
Affinity Capture-MS Homo sapiens
79 MYO18A 399687
Affinity Capture-MS Homo sapiens
80 ZNF280A  
Two-hybrid Homo sapiens
81 UBN2  
Affinity Capture-MS Homo sapiens
82 MAGED2 10916
Affinity Capture-MS Homo sapiens
83 CAPZA1 829
Affinity Capture-MS Homo sapiens
84 PINX1  
Affinity Capture-MS Homo sapiens
85 CUL4B 8450
Affinity Capture-MS Homo sapiens
86 RPS28 6234
Affinity Capture-MS Homo sapiens
87 MRPS34 65993
Affinity Capture-MS Homo sapiens
88 IQCG 84223
Two-hybrid Homo sapiens
89 RPS10 6204
Affinity Capture-MS Homo sapiens
90 LRRFIP2 9209
Affinity Capture-MS Homo sapiens
91 RALA 5898
Affinity Capture-MS Homo sapiens
92 PHKA1 5255
Affinity Capture-MS Homo sapiens
93 ZCCHC3  
Affinity Capture-MS Homo sapiens
94 TRIM24  
Affinity Capture-MS Homo sapiens
95 ANKS6 203286
Affinity Capture-MS Homo sapiens
96 BVES  
Affinity Capture-MS Homo sapiens
97 NSMF  
Two-hybrid Homo sapiens
98 KIAA1683  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
99 YAP1 10413
Affinity Capture-MS Homo sapiens
100 LRCH1 23143
Affinity Capture-MS Homo sapiens
101 ACTN4 81
Affinity Capture-MS Homo sapiens
102 MYO10 4651
Affinity Capture-Western Homo sapiens
103 TIGD1  
Affinity Capture-MS Homo sapiens
104 EFHD1  
Affinity Capture-MS Homo sapiens
105 IQCB1  
Affinity Capture-MS Homo sapiens
106 RPL36 25873
Affinity Capture-MS Homo sapiens
107 FLNB 2317
Affinity Capture-MS Homo sapiens
108 LYAR 55646
Affinity Capture-MS Homo sapiens
109 PML 5371
Affinity Capture-MS Homo sapiens
110 IQSEC2 23096
Affinity Capture-MS Homo sapiens
111 ARHGEF12 23365
Affinity Capture-MS Homo sapiens
112 ARPC5 10092
Affinity Capture-MS Homo sapiens
113 ATP2B4 493
Affinity Capture-MS Homo sapiens
114 POLB 5423
Affinity Capture-MS Homo sapiens
115 PELP1 27043
Affinity Capture-MS Homo sapiens
116 RPS15A 6210
Affinity Capture-MS Homo sapiens
117 CAMKK2 10645
Affinity Capture-MS Homo sapiens
118 DNM1L 10059
Affinity Capture-MS Homo sapiens
119 TOM1L2 146691
Affinity Capture-MS Homo sapiens
120 VPS13C 54832
Affinity Capture-MS Homo sapiens
121 LRCH3 84859
Affinity Capture-MS Homo sapiens
122 KIF1B 23095
Affinity Capture-MS Homo sapiens
123 CACNA1A  
Two-hybrid Homo sapiens
124 ITPR2 3709
Affinity Capture-MS Homo sapiens
125 CDC73  
Affinity Capture-MS Homo sapiens
126 CAMKK1  
Affinity Capture-MS Homo sapiens
127 LRRC59 55379
Affinity Capture-MS Homo sapiens
128 RC3H2  
Affinity Capture-MS Homo sapiens
129 DOCK7 85440
Affinity Capture-MS Homo sapiens
130 ATP2B1 490
Affinity Capture-MS Homo sapiens
131 CAMK1D 57118
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 LMO7 4008
Affinity Capture-MS Homo sapiens
133 RLIM 51132
Affinity Capture-MS Homo sapiens
134 MYL1 4632
Affinity Capture-MS Homo sapiens
135 PRKAR2A 5576
Affinity Capture-MS Homo sapiens
136 ASPM 259266
Affinity Capture-MS Homo sapiens
137 TJP1 7082
Affinity Capture-MS Homo sapiens
138 CUL3 8452
Affinity Capture-MS Homo sapiens
139 RPS7 6201
Affinity Capture-MS Homo sapiens
140 CCP110  
Affinity Capture-MS Homo sapiens
141 CTTN 2017
Affinity Capture-MS Homo sapiens
142 SPECC1 92521
Affinity Capture-MS Homo sapiens
143 PHKG2 5261
Affinity Capture-MS Homo sapiens
144 CD33 945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 HMBOX1  
Affinity Capture-MS Homo sapiens
146 OSTF1 26578
Affinity Capture-MS Homo sapiens
147 CEP290  
Affinity Capture-MS Homo sapiens
148 PSMD7 5713
Affinity Capture-MS Homo sapiens
149 MYO5C 55930
Affinity Capture-MS Homo sapiens
150 SPTBN1 6711
Affinity Capture-MS Homo sapiens
151 RPS4X 6191
Affinity Capture-MS Homo sapiens
152 NXF1 10482
Affinity Capture-RNA Homo sapiens
153 CDC42 998
Affinity Capture-MS Homo sapiens
154 LIN7A 8825
Affinity Capture-MS Homo sapiens
155 PDCD4 27250
Affinity Capture-MS Homo sapiens
156 RPS27 6232
Affinity Capture-MS Homo sapiens
157 DMAP1 55929
Affinity Capture-MS Homo sapiens
158 GADD45GIP1  
Affinity Capture-MS Homo sapiens
159 MYL6B 140465
Affinity Capture-MS Homo sapiens
160 MYO1D 4642
Affinity Capture-MS Homo sapiens
161 MYO6 4646
Affinity Capture-MS Homo sapiens
162 NEFM 4741
Affinity Capture-MS Homo sapiens
163 HECW2  
Affinity Capture-MS Homo sapiens
164 ESCO1  
Affinity Capture-MS Homo sapiens
165 PHKB 5257
Affinity Capture-MS Homo sapiens
166 DHX30 22907
Affinity Capture-MS Homo sapiens
167 DLST 1743
Affinity Capture-MS Homo sapiens
168 MYO9B 4650
Affinity Capture-MS Homo sapiens
169 SH2B1 25970
Affinity Capture-MS Homo sapiens
170 SCO1  
Affinity Capture-MS Homo sapiens
171 PSMC5 5705
Affinity Capture-MS Homo sapiens
172 HIP1 3092
Affinity Capture-MS Homo sapiens
173 AMOT 154796
Affinity Capture-MS Homo sapiens
174 KIAA1671  
Affinity Capture-MS Homo sapiens
175 AKAP2 11217
Affinity Capture-MS Homo sapiens
176 LRCH2  
Affinity Capture-MS Homo sapiens
177 MPRIP 23164
Affinity Capture-MS Homo sapiens
178 CETN2 1069
Affinity Capture-MS Homo sapiens
179 HMG20A  
Affinity Capture-MS Homo sapiens
180 CAMK2G 818
Affinity Capture-MS Homo sapiens
181 DDX49 54555
Affinity Capture-MS Homo sapiens
182 DDX55  
Affinity Capture-MS Homo sapiens
183 CCDC8  
Affinity Capture-MS Homo sapiens
184 DRD2  
Two-hybrid Homo sapiens
185 EIF3H 8667
Affinity Capture-MS Homo sapiens
186 ARPC1A 10552
Affinity Capture-MS Homo sapiens
187 PLEKHG3 26030
Affinity Capture-MS Homo sapiens
188 DENND4C 55667
Affinity Capture-MS Homo sapiens
189 RPL13 6137
Affinity Capture-MS Homo sapiens
190 ARPC3 10094
Affinity Capture-MS Homo sapiens
191 MYO1C 4641
Affinity Capture-MS Homo sapiens
192 UBE3B 89910
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
193 ARRB2 409
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
194 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
195 SPTAN1 6709
Affinity Capture-MS Homo sapiens
196 CCND2  
Reconstituted Complex Homo sapiens
197 DIDO1  
Affinity Capture-MS Homo sapiens
198 GRB2 2885
Affinity Capture-MS Homo sapiens
199 KTN1 3895
Affinity Capture-MS Homo sapiens
200 SVIL 6840
Affinity Capture-MS Homo sapiens
201 SSFA2 6744
Affinity Capture-MS Homo sapiens
202 ABHD10 55347
Affinity Capture-MS Homo sapiens
203 APEX1 328
Affinity Capture-RNA Homo sapiens
204 IQGAP3 128239
Affinity Capture-MS Homo sapiens
205 MRPL53  
Affinity Capture-MS Homo sapiens
206 PDHA1 5160
Affinity Capture-MS Homo sapiens
207 CAPZA2 830
Affinity Capture-MS Homo sapiens
208 ZNF536  
Affinity Capture-MS Homo sapiens
209 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
210 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
211 ITPR3 3710
Affinity Capture-MS Homo sapiens
212 HIST1H2BD 3017
Affinity Capture-MS Homo sapiens
213 ELFN2 114794
Affinity Capture-MS Homo sapiens
214 DDIT4L  
Two-hybrid Homo sapiens
215 MAP7D3 79649
Affinity Capture-MS Homo sapiens
216 UBE3C 9690
Affinity Capture-MS Homo sapiens
217 APPBP2  
Two-hybrid Homo sapiens
218 CHRM3 1131
Two-hybrid Homo sapiens
219 PHKA2 5256
Affinity Capture-MS Homo sapiens
220 PPP1R9A  
Affinity Capture-MS Homo sapiens
221 LIN7C 55327
Affinity Capture-MS Homo sapiens
222 CALM2 805
Affinity Capture-MS Homo sapiens
223 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
224 MOV10 4343
Affinity Capture-RNA Homo sapiens
225 INF2 64423
Affinity Capture-MS Homo sapiens
226 LRRK2 120892
Affinity Capture-MS Homo sapiens
227 DENND4A 10260
Affinity Capture-MS Homo sapiens
228 ARRB1 408
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
229 FLNA 2316
Affinity Capture-MS Homo sapiens
230 FECH 2235
Affinity Capture-MS Homo sapiens
231 MYO1E 4643
Affinity Capture-MS Homo sapiens
232 SPATA17  
Two-hybrid Homo sapiens
233 RPS27L 51065
Affinity Capture-MS Homo sapiens
234 PPP3CC 5533
Affinity Capture-MS Homo sapiens
235 CUL7 9820
Affinity Capture-MS Homo sapiens
236 CAMK2D 817
Affinity Capture-MS Homo sapiens
237 C9orf72  
Affinity Capture-MS Homo sapiens
238 WFS1 7466
Affinity Capture-MS Homo sapiens
239 KIF1C 10749
Affinity Capture-MS Homo sapiens
240 VIM 7431
Affinity Capture-MS Homo sapiens
241 RPL22L1 200916
Affinity Capture-MS Homo sapiens
242 RBM26 64062
Affinity Capture-MS Homo sapiens
243 ARPC4-TTLL3 100526693
Affinity Capture-MS Homo sapiens
244 UNC13B 10497
Affinity Capture-MS Homo sapiens
245 RACGAP1 29127
Affinity Capture-MS Homo sapiens
246 POC5  
Two-hybrid Homo sapiens
247 PNN 5411
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which CALM3 is involved
No pathways found





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