Gene description for UFSP2
Gene name UFM1-specific peptidase 2
Gene symbol UFSP2
Other names/aliases C4orf20
Species Homo sapiens
 Database cross references - UFSP2
ExoCarta ExoCarta_55325
Vesiclepedia VP_55325
Entrez Gene 55325
HGNC 25640
MIM 611482
UniProt Q9NUQ7  
 UFSP2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for UFSP2
Molecular Function
    protein binding GO:0005515 IPI
    deUFMylase activity GO:0071567 IBA
    deUFMylase activity GO:0071567 IDA
    deUFMylase activity GO:0071567 IMP
Biological Process
    proteolysis GO:0006508 IBA
    proteolysis GO:0006508 IMP
    regulation of type II interferon production GO:0032649 IEA
    ribosome disassembly GO:0032790 IDA
    regulation of intracellular estrogen receptor signaling pathway GO:0033146 IMP
    rescue of stalled ribosome GO:0072344 IDA
    negative regulation of proteolysis involved in protein catabolic process GO:1903051 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 ISS
    cytoplasm GO:0005737 ISS
    endoplasmic reticulum GO:0005783 IBA
    endoplasmic reticulum GO:0005783 IDA
 Experiment description of studies that identified UFSP2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for UFSP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LIG4 3981
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
2 C1orf27 54953
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
3 VAT1L 57687
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 SGCA  
Affinity Capture-MS Homo sapiens
5 CDH5  
Affinity Capture-MS Homo sapiens
6 TYROBP  
Affinity Capture-MS Homo sapiens
7 CBWD2  
Affinity Capture-MS Homo sapiens
8 SIGLEC5  
Affinity Capture-MS Homo sapiens
9 CD79A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 BTN3A3 10384
Affinity Capture-MS Homo sapiens
11 FAXC  
Affinity Capture-MS Homo sapiens
12 KIR3DL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 AMIGO1  
Affinity Capture-MS Homo sapiens
14 IGSF6  
Affinity Capture-MS Homo sapiens
15 HLA-B 3106
Affinity Capture-MS Homo sapiens
16 ASIC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 CPD 1362
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 GPRC5B 51704
Affinity Capture-MS Homo sapiens
19 AMIGO3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 COMT 1312
Affinity Capture-MS Homo sapiens
21 SLC51B  
Affinity Capture-MS Homo sapiens
22 SIGLEC12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 SLC16A11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 FAM177A1 283635
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 FAS 355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 KIR3DL3  
Affinity Capture-MS Homo sapiens
27 VSIG1  
Affinity Capture-MS Homo sapiens
28 BTN3A2 11118
Affinity Capture-MS Homo sapiens
29 ATG4A  
Affinity Capture-MS Homo sapiens
30 TMX1 81542
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 PARK2  
Affinity Capture-MS Homo sapiens
32 CEACAM21  
Affinity Capture-MS Homo sapiens
33 SLCO1B1  
Affinity Capture-MS Homo sapiens
34 ILVBL 10994
Affinity Capture-MS Homo sapiens
35 TIGIT  
Affinity Capture-MS Homo sapiens
36 CHMP6 79643
Affinity Capture-MS Homo sapiens
37 C16orf58 64755
Affinity Capture-MS Homo sapiens
38 CCDC107  
Affinity Capture-MS Homo sapiens
39 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
40 IL27RA 9466
Affinity Capture-MS Homo sapiens
41 CLSTN2 64084
Affinity Capture-MS Homo sapiens
42 PLXDC2 84898
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 KCNJ6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 NAPG 8774
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 GRIN2A  
Affinity Capture-MS Homo sapiens
46 ADAM18  
Affinity Capture-MS Homo sapiens
47 C17orf62 79415
Affinity Capture-MS Homo sapiens
48 SEC61B 10952
Affinity Capture-MS Homo sapiens
49 PCDHA4 56144
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ARFGAP1 55738
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 CLRN2  
Affinity Capture-MS Homo sapiens
52 KDF1  
Affinity Capture-MS Homo sapiens
53 REEP5 7905
Affinity Capture-MS Homo sapiens
54 MCOLN2  
Affinity Capture-MS Homo sapiens
55 DUOXA2  
Affinity Capture-MS Homo sapiens
56 ASCC1 51008
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
57 TMEM9B 56674
Affinity Capture-MS Homo sapiens
58 TCL1A  
Affinity Capture-MS Homo sapiens
59 C9orf9  
Affinity Capture-MS Homo sapiens
60 EGFR 1956
Negative Genetic Homo sapiens
61 FAM84B 157638
Affinity Capture-MS Homo sapiens
62 CD27  
Affinity Capture-MS Homo sapiens
63 SAAL1 113174
Affinity Capture-MS Homo sapiens
64 NDRG4 65009
Affinity Capture-MS Homo sapiens
65 FAM134C 162427
Affinity Capture-MS Homo sapiens
66 RPA3 6119
Proximity Label-MS Homo sapiens
67 LRIG1 26018
Affinity Capture-MS Homo sapiens
68 EMC2 9694
Affinity Capture-MS Homo sapiens
69 CD244 51744
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 PCDHA12  
Affinity Capture-MS Homo sapiens
71 CDHR5 53841
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 DDRGK1 65992
Affinity Capture-MS Homo sapiens
73 APOBEC1  
Two-hybrid Homo sapiens
74 LPPR2  
Affinity Capture-MS Homo sapiens
75 SAYSD1  
Affinity Capture-MS Homo sapiens
76 FMR1NB  
Affinity Capture-MS Homo sapiens
77 RIT1 6016
Negative Genetic Homo sapiens
78 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
79 OSTM1 28962
Affinity Capture-MS Homo sapiens
80 ASIC4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 NPL 80896
Affinity Capture-MS Homo sapiens
82 PODXL2 50512
Affinity Capture-MS Homo sapiens
83 DLK1  
Affinity Capture-MS Homo sapiens
84 SLAMF1 6504
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
85 HM13 81502
Affinity Capture-MS Homo sapiens
86 SEC62 7095
Affinity Capture-MS Homo sapiens
87 PTPRCAP 5790
Affinity Capture-MS Homo sapiens
88 SORT1 6272
Affinity Capture-MS Homo sapiens
89 RAB33B 83452
Affinity Capture-MS Homo sapiens
90 LMO1  
Two-hybrid Homo sapiens
91 VASN 114990
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 HLA-C 3107
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
93 PCDHB3  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which UFSP2 is involved
No pathways found





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