Gene description for ALDH3B1
Gene name aldehyde dehydrogenase 3 family, member B1
Gene symbol ALDH3B1
Other names/aliases ALDH4
ALDH7
Species Homo sapiens
 Database cross references - ALDH3B1
ExoCarta ExoCarta_221
Vesiclepedia VP_221
Entrez Gene 221
HGNC 410
MIM 600466
UniProt P43353  
 ALDH3B1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Tracheobronchial cells 19190083    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for ALDH3B1
Molecular Function
    3-chloroallyl aldehyde dehydrogenase activity GO:0004028 IBA
    aldehyde dehydrogenase (NAD+) activity GO:0004029 IBA
    aldehyde dehydrogenase [NAD(P)+] activity GO:0004030 IDA
    aldehyde dehydrogenase [NAD(P)+] activity GO:0004030 TAS
    protein binding GO:0005515 IPI
    benzaldehyde dehydrogenase (NADP+) activity GO:0018477 IEA
    benzaldehyde dehydrogenase (NAD+) activity GO:0018479 IEA
    long-chain fatty aldehyde dehydrogenase (NAD+) activity GO:0050061 IEA
    medium-chain fatty aldehyde dehydrogenase (NAD+) activity GO:0052814 IEA
Biological Process
    alcohol metabolic process GO:0006066 TAS
    ethanol catabolic process GO:0006068 IEA
    aldehyde metabolic process GO:0006081 IBA
    lipid metabolic process GO:0006629 TAS
    response to oxidative stress GO:0006979 IDA
    sphingolipid catabolic process GO:0030149 TAS
    cellular response to oxidative stress GO:0034599 IDA
    aldehyde catabolic process GO:0046185 IDA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IEA
    plasma membrane GO:0005886 TAS
    secretory granule membrane GO:0030667 TAS
    vesicle GO:0031982 HDA
    specific granule membrane GO:0035579 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified ALDH3B1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 34
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
TSG101|CD63|MHCI|MHCII
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19190083    
Organism Homo sapiens
Experiment description Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: a possible role in innate defense.
Authors "Kesimer M, Scull M, Brighton B, Demaria G, Burns K, O'Neal W, Pickles RJ, Sheehan JK"
Journal name FASEB
Publication year 2009
Sample Tracheobronchial cells
Sample name Tracheobronchial epithelial cells
Isolation/purification methods Differential centrifugation
Filtration
Sucrose density gradient
Flotation density 1.16 -1.18 g/mL
Molecules identified in the study Protein
mRNA
Methods used in the study Mass spectrometry [QTOF]
Western blotting
Immunoelectron Microscopy
FACS
8
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
9
Experiment ID 196
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 197
MISEV standards
✔
EM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors "Raj DA, Fiume I, Capasso G, Pocsfalvi G."
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ALDH3B1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 RAMP3  
Affinity Capture-MS Homo sapiens
3 WDR6 11180
Affinity Capture-MS Homo sapiens
4 EIF2AK4 440275
Affinity Capture-MS Homo sapiens
5 TRIM65 201292
Affinity Capture-MS Homo sapiens
6 MTR 4548
Affinity Capture-MS Homo sapiens
7 FAS 355
Affinity Capture-MS Homo sapiens
8 TLK2  
Affinity Capture-MS Homo sapiens
9 ASTN2  
Affinity Capture-MS Homo sapiens
10 HSPA1L 3305
Affinity Capture-MS Homo sapiens
11 CBWD2  
Affinity Capture-MS Homo sapiens
12 FMN2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 FAM174A 345757
Affinity Capture-MS Homo sapiens
14 WDTC1 23038
Affinity Capture-MS Homo sapiens
15 ALG3 10195
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TAF9 6880
Affinity Capture-MS Homo sapiens
17 HSPA2 3306
Affinity Capture-MS Homo sapiens
18 NAGA 4668
Affinity Capture-MS Homo sapiens
19 KRT40  
Two-hybrid Homo sapiens
20 KRTAP4-2  
Two-hybrid Homo sapiens
21 OBSL1 23363
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 CHIT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 PPM1A 5494
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 ATG7 10533
Affinity Capture-MS Homo sapiens
25 RNF123 63891
Affinity Capture-MS Homo sapiens
26 UBAC1 10422
Affinity Capture-MS Homo sapiens
27 HSPA8 3312
Affinity Capture-MS Homo sapiens
28 GPN3  
Affinity Capture-MS Homo sapiens
29 NUP155 9631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 BAG5 9529
Affinity Capture-MS Homo sapiens
31 SEZ6L2 26470
Affinity Capture-MS Homo sapiens
32 TCP1 6950
Affinity Capture-MS Homo sapiens
33 MARCH7  
Affinity Capture-MS Homo sapiens
34 CHIA  
Affinity Capture-MS Homo sapiens
35 WDR73  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 KRT31 3881
Two-hybrid Homo sapiens
37 B4GALT2  
Affinity Capture-MS Homo sapiens
38 BLM 641
Synthetic Growth Defect Homo sapiens
39 TUBA1A 7846
Affinity Capture-MS Homo sapiens
40 TUBA1C 84790
Affinity Capture-MS Homo sapiens
41 APOL2 23780
Affinity Capture-MS Homo sapiens
42 GPR182  
Affinity Capture-MS Homo sapiens
43 NOS2  
Affinity Capture-MS Homo sapiens
44 NME3 4832
Affinity Capture-MS Homo sapiens
45 GNAI2 2771
Affinity Capture-MS Homo sapiens
46 CTR9 9646
Affinity Capture-MS Homo sapiens
47 GFAP 2670
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 CACNG4  
Affinity Capture-MS Homo sapiens
49 TNFRSF10A 8797
Affinity Capture-MS Homo sapiens
50 USP32 84669
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 NMNAT2  
Affinity Capture-MS Homo sapiens
52 KRTAP10-7  
Two-hybrid Homo sapiens
53 ZMYM4  
Affinity Capture-MS Homo sapiens
54 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
55 NOTCH2NL 388677
Two-hybrid Homo sapiens
56 SELT 51714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 SPHK1 8877
Affinity Capture-MS Homo sapiens
58 NUBP2 10101
Affinity Capture-MS Homo sapiens
59 LILRA3  
Affinity Capture-MS Homo sapiens
60 KRTAP10-8  
Two-hybrid Homo sapiens
61 MADD 8567
Affinity Capture-MS Homo sapiens
62 HSD17B10 3028
Two-hybrid Homo sapiens
63 KRTAP10-3  
Two-hybrid Homo sapiens
64 SBF1 6305
Affinity Capture-MS Homo sapiens
65 FBXL12 54850
Affinity Capture-Western Homo sapiens
66 TUBA4A 7277
Affinity Capture-MS Homo sapiens
67 TANGO6 79613
Affinity Capture-MS Homo sapiens
68 LACRT 90070
Affinity Capture-MS Homo sapiens
69 KIF1BP 26128
Affinity Capture-MS Homo sapiens
70 UHRF1BP1 54887
Affinity Capture-MS Homo sapiens
71 ALDH3B2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 METTL21B  
Affinity Capture-MS Homo sapiens
73 PRSS8 5652
Affinity Capture-MS Homo sapiens
74 CDK20  
Affinity Capture-MS Homo sapiens
75 TSC22D3  
Affinity Capture-MS Homo sapiens
76 SLC9A6 10479
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 PASK  
Affinity Capture-MS Homo sapiens
78 KRTAP10-5  
Two-hybrid Homo sapiens
79 STUB1 10273
Affinity Capture-MS Homo sapiens
80 HK2 3099
Affinity Capture-MS Homo sapiens
81 TARSL2 123283
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 NSUN5P1  
Affinity Capture-MS Homo sapiens
83 GP9 2815
Affinity Capture-MS Homo sapiens
84 PPIP5K1 9677
Affinity Capture-MS Homo sapiens
85 IREB2 3658
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 DNAJA2 10294
Affinity Capture-MS Homo sapiens
87 COQ5  
Affinity Capture-MS Homo sapiens
88 VWA9  
Affinity Capture-MS Homo sapiens
89 WRAP53  
Affinity Capture-MS Homo sapiens
90 DMWD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 TUBB1 81027
Affinity Capture-MS Homo sapiens
92 INF2 64423
Affinity Capture-MS Homo sapiens
93 CBWD3  
Affinity Capture-MS Homo sapiens
94 PIK3R2 5296
Affinity Capture-MS Homo sapiens
95 UBA6 55236
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
96 SLC1A1 6505
Affinity Capture-MS Homo sapiens
97 HSPA1B 3304
Affinity Capture-MS Homo sapiens
98 PIK3R3 8503
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 KRTAP10-9  
Two-hybrid Homo sapiens
View the network image/svg+xml



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