Gene description for RHOBTB3
Gene name Rho-related BTB domain containing 3
Gene symbol RHOBTB3
Other names/aliases -
Species Homo sapiens
 Database cross references - RHOBTB3
ExoCarta ExoCarta_22836
Vesiclepedia VP_22836
Entrez Gene 22836
HGNC 18757
MIM 607353
UniProt O94955  
 RHOBTB3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RHOBTB3
Molecular Function
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IDA
    ATP hydrolysis activity GO:0016887 IDA
    ATP hydrolysis activity GO:0016887 TAS
    small GTPase binding GO:0031267 IPI
    ubiquitin protein ligase binding GO:0031625 IBA
Biological Process
    male gonad development GO:0008584 IEA
    regulation of proteolysis GO:0030162 IBA
    retrograde transport, endosome to Golgi GO:0042147 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IEA
    trans-Golgi network membrane GO:0032588 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified RHOBTB3 in exosomes
1
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RHOBTB3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADAM11 4185
Affinity Capture-MS Homo sapiens
2 KLK15  
Affinity Capture-MS Homo sapiens
3 RBPMS 11030
Two-hybrid Homo sapiens
4 CD3D 915
Affinity Capture-MS Homo sapiens
5 CCDC36  
Two-hybrid Homo sapiens
6 SSR2  
Affinity Capture-MS Homo sapiens
7 HCST  
Affinity Capture-MS Homo sapiens
8 RAMP3  
Affinity Capture-MS Homo sapiens
9 TRIM66  
Affinity Capture-MS Homo sapiens
10 PBK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 RBX1 9978
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 EGLN1 54583
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 VHL  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 RABGGTB 5876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 GP5 2814
Affinity Capture-MS Homo sapiens
16 TMEM130  
Affinity Capture-MS Homo sapiens
17 HGS 9146
Reconstituted Complex Homo sapiens
18 NUP210 23225
Affinity Capture-MS Homo sapiens
19 THTPA  
Affinity Capture-MS Homo sapiens
20 KLRC1  
Affinity Capture-MS Homo sapiens
21 PIGH  
Affinity Capture-MS Homo sapiens
22 HTR7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
23 DGCR2 9993
Affinity Capture-MS Homo sapiens
24 ATG7 10533
Affinity Capture-MS Homo sapiens
25 IL1R2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PCYOX1 51449
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 FAS 355
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 KLK5 25818
Affinity Capture-MS Homo sapiens
29 IGDCC4  
Affinity Capture-MS Homo sapiens
30 BSG 682
Affinity Capture-MS Homo sapiens
31 EFNA2  
Affinity Capture-MS Homo sapiens
32 CX3CL1  
Affinity Capture-MS Homo sapiens
33 RHOBTB2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
34 Lrrc41  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
35 ILVBL 10994
Affinity Capture-MS Homo sapiens
36 TIGIT  
Affinity Capture-MS Homo sapiens
37 CCNB1 891
Affinity Capture-Western Homo sapiens
38 IL2RA  
Affinity Capture-MS Homo sapiens
39 NTRK1 4914
Affinity Capture-MS Homo sapiens
40 GPR182  
Affinity Capture-MS Homo sapiens
41 TSPAN31 6302
Affinity Capture-MS Homo sapiens
42 FURIN 5045
Affinity Capture-MS Homo sapiens
43 ARRDC4  
Affinity Capture-MS Homo sapiens
44 CD83  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 TNFSF8  
Affinity Capture-MS Homo sapiens
46 LRRC25  
Affinity Capture-MS Homo sapiens
47 GALNT6 11226
Affinity Capture-MS Homo sapiens
48 RTN4R 65078
Affinity Capture-MS Homo sapiens
49 BHLHE40  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 CUL3 8452
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
51 CCNE1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
52 USP54 159195
Two-hybrid Homo sapiens
53 KLRD1  
Affinity Capture-MS Homo sapiens
54 METTL21B  
Affinity Capture-MS Homo sapiens
55 ZP2  
Affinity Capture-MS Homo sapiens
56 KCNE3  
Affinity Capture-MS Homo sapiens
57 L3MBTL3  
Two-hybrid Homo sapiens
58 RELT  
Affinity Capture-MS Homo sapiens
59 RAB9A 9367
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
60 PLIN3 10226
Co-purification Homo sapiens
61 LAMP3  
Affinity Capture-MS Homo sapiens
62 ISLR 3671
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 HUWE1 10075
Affinity Capture-MS Homo sapiens
64 HIF1A 3091
Affinity Capture-Western Homo sapiens
65 NRG1 3084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 CA6 765
Affinity Capture-MS Homo sapiens
67 IL17RB  
Affinity Capture-MS Homo sapiens
68 TMEM9 252839
Affinity Capture-MS Homo sapiens
69 EFHC2  
Two-hybrid Homo sapiens
70 RHOBTB3 22836
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
71 PLAGL2  
Two-hybrid Homo sapiens
72 OPALIN  
Affinity Capture-MS Homo sapiens
73 LDLRAD1  
Affinity Capture-MS Homo sapiens
74 DNAJA2 10294
Affinity Capture-MS Homo sapiens
75 CLEC2D  
Affinity Capture-MS Homo sapiens
76 ARF5 381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
77 HAVCR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 UBXN11  
Two-hybrid Homo sapiens
79 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
80 IL4R  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 KIR2DS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 C16orf72 29035
Affinity Capture-MS Homo sapiens
83 TNFRSF9  
Affinity Capture-MS Homo sapiens
84 LIMD1 8994
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
85 KIR2DL4  
Affinity Capture-MS Homo sapiens
86 VIPR1  
Affinity Capture-MS Homo sapiens
87 HLA-C 3107
Affinity Capture-MS Homo sapiens
88 KCNE4  
Affinity Capture-MS Homo sapiens
89 PCDHB3  
Affinity Capture-MS Homo sapiens
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