ExoCarta: Gene summary

Credits | Contact

Gene description for CETN2
Gene name	centrin, EF-hand protein, 2
Gene symbol	CETN2
Other names/aliases	CALT CEN2
Species	Homo sapiens
Database cross references - CETN2
ExoCarta	ExoCarta_1069
Vesiclepedia	VP_1069
Entrez Gene	1069
HGNC	1867
MIM	300006
UniProt	P41208
CETN2 identified in exosomes derived from the following tissue/cell type
Gastric cancer cells	33991177
Gastric cancer cells	33991177
Gastric cancer cells	33991177

Gene ontology annotations for CETN2

Molecular Function
	calcium ion binding	GO:0005509	IBA
	calcium ion binding	GO:0005509	NAS
	protein binding	GO:0005515	IPI
	microtubule binding	GO:0008017	IEA
	G-protein beta/gamma-subunit complex binding	GO:0031683	IEA
	heterotrimeric G-protein binding	GO:0032795	IEA
Biological Process
	microtubule cytoskeleton organization	GO:0000226	IBA
	mitotic cell cycle	GO:0000278	NAS
	nucleotide-excision repair	GO:0006289	IBA
	nucleotide-excision repair	GO:0006289	IDA
	centriole replication	GO:0007099	IBA
	centriole replication	GO:0007099	IMP
	spermatogenesis	GO:0007283	IEA
	protein transport	GO:0015031	IEA
	regulation of cytokinesis	GO:0032465	IMP
	mRNA transport	GO:0051028	IEA
	cell division	GO:0051301	IEA
Subcellular Localization
	nucleus	GO:0005634	IBA
	nucleoplasm	GO:0005654	TAS
	centrosome	GO:0005813	IBA
	centrosome	GO:0005813	IDA
	centrosome	GO:0005813	NAS
	centriole	GO:0005814	IBA
	centriole	GO:0005814	IDA
	cytosol	GO:0005829	TAS
	photoreceptor connecting cilium	GO:0032391	IEA
	ciliary basal body	GO:0036064	IEA
	nuclear pore nuclear basket	GO:0044615	IDA
	apical part of cell	GO:0045177	IEA
	transcription export complex 2	GO:0070390	IDA
	XPC complex	GO:0071942	IDA
	9+2 motile cilium	GO:0097729	IEA

Experiment description of studies that identified CETN2 in exosomes

Experiment ID

363

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

364

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Experiment ID

365

MISEV standards
✘	Biophysical techniques
✔ TSG101\|GAPDH\|AQP1\|CD151\|CD81\|CD82\|CD9\|EPCAM\|FLOT1\|FLOT2\|ICAM1\|ITGA2B\|LAMP2\|RAB35\|RAB5A\|RAB5B\|SDCBP\|TFRC\|UCHL1	Enriched markers
✔ DCLK1	Negative markers
✔ NTA	Particle analysis

Identified molecule

Protein

Identification method

Mass spectrometry

PubMed ID

33991177

Organism

Homo sapiens

Experiment description

Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells

Authors

"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."

Journal name

Proteomics

Publication year

2021

Sample

Gastric cancer cells

Sample name

MKN1 - 100K pellet

Isolation/purification methods

Differential centrifugation
Ultracentrifugation

Flotation density

Molecules identified in the study

Protein

Methods used in the study

Western blotting
Mass spectrometry

Protein-protein interactions for CETN2

Protein Interactor

ExoCarta ID

Identification method

PubMed

Species

CDCA5

Co-fractionation

Homo sapiens

XPC

Co-purification		Homo sapiens
Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Reconstituted Complex		Homo sapiens
Co-fractionation		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-fractionation		Homo sapiens

CCNH

Affinity Capture-MS

Homo sapiens

BCKDK

10295

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

MACROD1

28992

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ERCC2

2068

Affinity Capture-MS

Homo sapiens

NAA40

Co-fractionation

Homo sapiens

RPA2

6118

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HIST1H2BG

8339

Affinity Capture-MS

Homo sapiens

TSNAX

7257

Affinity Capture-MS

Homo sapiens

PRMT6

Two-hybrid

Homo sapiens

CALM1

801

Affinity Capture-MS

Homo sapiens

NUP160

23279

Affinity Capture-Western		Homo sapiens
Affinity Capture-Western		Homo sapiens

WDR76

Affinity Capture-MS

Homo sapiens

Cetn2

Affinity Capture-MS

Mus musculus

DCAF12L2

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

USP39

10713

Co-fractionation

Homo sapiens

NUP153

9972

Affinity Capture-Western

Homo sapiens

IMPDH2

3615

Co-fractionation

Homo sapiens

CALM3

808

Affinity Capture-MS

Homo sapiens

POU5F1

Affinity Capture-Western

Homo sapiens

FBXL13

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SFI1

9814

Reconstituted Complex		Homo sapiens
Affinity Capture-MS		Homo sapiens

HIST2H2AC

8338

Affinity Capture-MS

Homo sapiens

DNHD1

Affinity Capture-MS

Homo sapiens

XIRP1

Affinity Capture-MS

Homo sapiens

PKN2

5586

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

RAD23A

5886

Affinity Capture-MS

Homo sapiens

FAS

355

Proximity Label-MS

Homo sapiens

Wiz

Affinity Capture-MS

Mus musculus

CALM2

805

Affinity Capture-MS

Homo sapiens

RAD23B

5887

Affinity Capture-Western		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HIST1H4A

8359

Affinity Capture-MS

Homo sapiens

Pabpc1

18458

Affinity Capture-MS

Mus musculus

FAM96B

51647

Affinity Capture-MS

Homo sapiens

PNMA5

114824

Two-hybrid

Homo sapiens

RNF146

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FBXO16

Affinity Capture-MS

Homo sapiens

HIST1H2AM

8336

Affinity Capture-MS

Homo sapiens

GTF2H1

2965

Affinity Capture-MS

Homo sapiens

MNAT1

Affinity Capture-MS

Homo sapiens

HIST1H1E

3008

Co-fractionation

Homo sapiens

PNMA2

Affinity Capture-MS

Homo sapiens

KIF11

3832

Affinity Capture-MS

Homo sapiens

CTR9

9646

Affinity Capture-MS

Homo sapiens

POLB

5423

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FGF4

Affinity Capture-MS

Homo sapiens

HIST1H2AB

8335

Affinity Capture-MS

Homo sapiens

PIPSL

266971

Affinity Capture-MS

Homo sapiens

EIF4A2

1974

Co-fractionation

Homo sapiens

CETN1

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

ARSA

410

Two-hybrid

Homo sapiens

HNRNPD

3184

Affinity Capture-MS

Homo sapiens

NUP37

79023

Affinity Capture-Western

Homo sapiens

CDK7

1022

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

LRRC39

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

PITHD1

57095

Co-fractionation

Homo sapiens

CUL3

8452

Affinity Capture-MS

Homo sapiens

NUP43

348995

Affinity Capture-Western

Homo sapiens

EFCAB11

Affinity Capture-MS

Homo sapiens

CCDC127

Affinity Capture-MS

Homo sapiens

HIST1H3B

8358

Affinity Capture-MS

Homo sapiens

MYC

Affinity Capture-MS

Homo sapiens

HIST1H2BA

255626

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SGSM1

Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens

ATG14

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HIST1H3I

8354

Affinity Capture-MS

Homo sapiens

CCDC88A

55704

Affinity Capture-MS

Homo sapiens

CDC7

Affinity Capture-MS

Homo sapiens

APLF

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

IL6R

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

HIST1H2BI

8346

Affinity Capture-MS

Homo sapiens

NUP133

55746

Affinity Capture-Western

Homo sapiens

H2AFX

3014

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

FTL

2512

Affinity Capture-MS

Homo sapiens

AGAP3

116988

Affinity Capture-MS

Homo sapiens

PARP1

142

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

USP49

25862

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

SRRM1

10250

Cross-Linking-MS (XL-MS)

Homo sapiens

CCHCR1

Affinity Capture-MS

Homo sapiens

Cep135

Affinity Capture-MS

Mus musculus

CETN3

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

USP44

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Co-purification		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

GTF2H5

Affinity Capture-MS

Homo sapiens

FAM35A

Proximity Label-MS

Homo sapiens

H2AFB3

Affinity Capture-MS

Homo sapiens

ERCC3

Affinity Capture-MS

Homo sapiens

HIST2H2AA4

723790

Affinity Capture-MS

Homo sapiens

GTF2H3

Affinity Capture-MS

Homo sapiens

GTF2H4

2968

Affinity Capture-MS

Homo sapiens

Brca1

Affinity Capture-MS

Mus musculus

UBA6

55236

Co-fractionation

Homo sapiens

NUP85

79902

Affinity Capture-Western

Homo sapiens

HIST1H2AE

3012

Affinity Capture-MS

Homo sapiens

POC5

Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens
Two-hybrid		Homo sapiens
Two-hybrid		Homo sapiens
Affinity Capture-MS		Homo sapiens

DNAH14

Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens
Affinity Capture-MS		Homo sapiens

View the network

Pathways in which CETN2 is involved

Pathway	Evidence	Source
Anchoring of the basal body to the plasma membrane	TAS	Reactome
AURKA Activation by TPX2	TAS	Reactome
Cell Cycle	TAS	Reactome
Cell Cycle, Mitotic	TAS	Reactome
Centrosome maturation	TAS	Reactome
Cilium Assembly	TAS	Reactome
DNA Damage Recognition in GG-NER	TAS	Reactome
DNA Repair	TAS	Reactome
Formation of Incision Complex in GG-NER	TAS	Reactome
G2/M Transition	TAS	Reactome
Global Genome Nucleotide Excision Repair (GG-NER)	TAS	Reactome
Loss of Nlp from mitotic centrosomes	TAS	Reactome
Loss of proteins required for interphase microtubule organization from the centrosome	TAS	Reactome
M Phase	TAS	Reactome
Metabolism of proteins	TAS	Reactome
Mitotic G2-G2/M phases	TAS	Reactome
Mitotic Prometaphase	TAS	Reactome
Nucleotide Excision Repair	TAS	Reactome
Organelle biogenesis and maintenance	TAS	Reactome
Post-translational protein modification	TAS	Reactome
Recruitment of mitotic centrosome proteins and complexes	TAS	Reactome
Recruitment of NuMA to mitotic centrosomes	TAS	Reactome
Regulation of PLK1 Activity at G2/M Transition	TAS	Reactome
SUMO E3 ligases SUMOylate target proteins	TAS	Reactome
SUMOylation	TAS	Reactome
SUMOylation of DNA damage response and repair proteins	TAS	Reactome

Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here