Gene description for ARL4C
Gene name ADP-ribosylation factor-like 4C
Gene symbol ARL4C
Other names/aliases ARL7
LAK
Species Homo sapiens
 Database cross references - ARL4C
ExoCarta ExoCarta_10123
Vesiclepedia VP_10123
Entrez Gene 10123
HGNC 698
MIM 604787
UniProt P56559  
 ARL4C identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for ARL4C
Molecular Function
    GTPase activity GO:0003924 IEA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IBA
    GTP binding GO:0005525 IEA
    alpha-tubulin binding GO:0043014 IDA
Biological Process
    intracellular protein transport GO:0006886 IBA
    endocytic recycling GO:0032456 IBA
    endocytic recycling GO:0032456 IDA
Subcellular Localization
    nucleus GO:0005634 TAS
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    filopodium GO:0030175 IEA
 Experiment description of studies that identified ARL4C in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
4
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for ARL4C
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RAD23A 5886
Affinity Capture-MS Homo sapiens
2 POLE4  
Affinity Capture-MS Homo sapiens
3 RAP1GDS1 5910
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TERF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 UBQLN4 56893
Two-hybrid Homo sapiens
6 RGS12 6002
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 POLE2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 FBXO33  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 FAS 355
Affinity Capture-MS Homo sapiens
10 XRCC2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 DDX19B 11269
Affinity Capture-MS Homo sapiens
12 MISP 126353
Affinity Capture-MS Homo sapiens
13 ARF5 381
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 RBP4 5950
Affinity Capture-MS Homo sapiens
15 RAD51B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 TSEN2  
Affinity Capture-MS Homo sapiens
17 CBWD1 55871
Affinity Capture-MS Homo sapiens
18 TERF2IP 54386
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 EDC3 80153
Affinity Capture-MS Homo sapiens
20 APP 351
Reconstituted Complex Homo sapiens
21 GAB1  
Affinity Capture-MS Homo sapiens
22 RAD51D  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 NKIRAS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 DIRAS1 148252
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PTPRK 5796
Affinity Capture-MS Homo sapiens
26 DCUN1D4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 OAF 220323
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PAF1 54623
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 DNAJC12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 APIP 51074
Affinity Capture-MS Homo sapiens
31 ARL10  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 TUBB8 347688
Affinity Capture-MS Homo sapiens
33 LOC388882  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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