Gene description for MPI
Gene name mannose phosphate isomerase
Gene symbol MPI
Other names/aliases CDG1B
PMI
PMI1
Species Homo sapiens
 Database cross references - MPI
ExoCarta ExoCarta_4351
Vesiclepedia VP_4351
Entrez Gene 4351
HGNC 7216
MIM 154550
UniProt P34949  
 MPI identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for MPI
Molecular Function
    mannose-6-phosphate isomerase activity GO:0004476 IBA
    mannose-6-phosphate isomerase activity GO:0004476 IDA
    mannose-6-phosphate isomerase activity GO:0004476 IMP
    mannose-6-phosphate isomerase activity GO:0004476 TAS
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
Biological Process
    GDP-mannose biosynthetic process GO:0009298 IBA
    GDP-mannose biosynthetic process GO:0009298 TAS
    mannose to fructose-6-phosphate catabolic process GO:0061611 IDA
    mannose to fructose-6-phosphate catabolic process GO:0061611 IMP
    GDP-mannose biosynthetic process from fructose-6-phosphate GO:0061729 IMP
    GDP-mannose biosynthetic process from glucose GO:0141199 IEA
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified MPI in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MPI
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLHL9 55958
Affinity Capture-MS Homo sapiens
2 APP 351
Reconstituted Complex Homo sapiens
3 ANLN 54443
Affinity Capture-MS Homo sapiens
4 HSPE1 3336
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
5 FAM185A  
Co-fractionation Homo sapiens
6 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
7 MAGEF1  
Affinity Capture-MS Homo sapiens
8 TIA1 7072
Co-fractionation Homo sapiens
9 IVL 3713
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SOD1 6647
Co-fractionation Homo sapiens
11 FAS 355
Affinity Capture-MS Homo sapiens
12 CCDC113  
Affinity Capture-MS Homo sapiens
13 RIT1 6016
Negative Genetic Homo sapiens
14 RPA2 6118
Proximity Label-MS Homo sapiens
15 SERPINB4 6318
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 CUL3 8452
Affinity Capture-MS Homo sapiens
17 PPCS 79717
Affinity Capture-MS Homo sapiens
18 NANS 54187
Co-fractionation Homo sapiens
19 ADA 100
Affinity Capture-MS Homo sapiens
20 OPTN 10133
Affinity Capture-MS Homo sapiens
21 KLHL13 90293
Affinity Capture-MS Homo sapiens
22 S100A14 57402
Affinity Capture-MS Homo sapiens
23 MIF 4282
Co-fractionation Homo sapiens
24 TMEM173  
Affinity Capture-MS Homo sapiens
25 NECAP2 55707
Co-fractionation Homo sapiens
26 PCBP1 5093
Co-fractionation Homo sapiens
27 RPA3 6119
Proximity Label-MS Homo sapiens
28 CKAP5 9793
Co-fractionation Homo sapiens
29 PITPNB 23760
Co-fractionation Homo sapiens
30 SAR1B 51128
Affinity Capture-MS Homo sapiens
31 DAZAP1 26528
Co-fractionation Homo sapiens
32 SERBP1 26135
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 CAPN9  
Co-fractionation Homo sapiens
34 GMPPB 29925
Co-fractionation Homo sapiens
35 BDKRB2  
Two-hybrid Homo sapiens
36 TGM1 7051
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 TKFC 26007
Affinity Capture-MS Homo sapiens
38 CPNE8 144402
Affinity Capture-MS Homo sapiens
39 NECAP1 25977
Co-fractionation Homo sapiens
40 SERPINA3 12
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 P2RY10  
Affinity Capture-MS Homo sapiens
42 NEDD8 4738
Co-fractionation Homo sapiens
43 SRI 6717
Co-fractionation Homo sapiens
View the network image/svg+xml



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