Gene description for CCS
Gene name copper chaperone for superoxide dismutase
Gene symbol CCS
Other names/aliases -
Species Homo sapiens
 Database cross references - CCS
ExoCarta ExoCarta_9973
Vesiclepedia VP_9973
Entrez Gene 9973
HGNC 1613
MIM 603864
UniProt O14618  
 CCS identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CCS
Molecular Function
    copper ion binding GO:0005507 IBA
    copper ion binding GO:0005507 IDA
    protein binding GO:0005515 IPI
    protein-disulfide reductase activity GO:0015035 TAS
    superoxide dismutase copper chaperone activity GO:0016532 IBA
    cadherin binding GO:0045296 HDA
Biological Process
    superoxide metabolic process GO:0006801 TAS
    protein maturation by copper ion transfer GO:0015680 TAS
    removal of superoxide radicals GO:0019430 IBA
    cellular response to oxidative stress GO:0034599 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 HTP
    cytosol GO:0005829 TAS
 Experiment description of studies that identified CCS in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CCS
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NIT2 56954
Co-fractionation Homo sapiens
2 NIF3L1 60491
Co-fractionation Homo sapiens
3 RIT1 6016
Negative Genetic Homo sapiens
4 APOA1BP 128240
Co-fractionation Homo sapiens
5 PMPCA 23203
Co-fractionation Homo sapiens
6 GSTO1 9446
Co-fractionation Homo sapiens
7 ACSF2 80221
Co-fractionation Homo sapiens
8 XIAP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 GMPPB 29925
Co-fractionation Homo sapiens
10 SOD1 6647
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
11 RAE1 8480
Co-fractionation Homo sapiens
12 HSD17B7 51478
Co-fractionation Homo sapiens
13 FAS 355
Proximity Label-MS Homo sapiens
14 SUCLG2 8801
Co-fractionation Homo sapiens
15 PPA1 5464
Co-fractionation Homo sapiens
16 ANXA5 308
Co-fractionation Homo sapiens
17 DNAL4  
Affinity Capture-MS Homo sapiens
18 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 PCK2 5106
Co-fractionation Homo sapiens
20 SUCLG1 8802
Co-fractionation Homo sapiens
21 SSB 6741
Co-fractionation Homo sapiens
22 CUL4B 8450
Affinity Capture-MS Homo sapiens
23 SOX2  
Affinity Capture-MS Homo sapiens
24 DUSP13  
Affinity Capture-MS Homo sapiens
25 PPIL3 53938
Co-fractionation Homo sapiens
26 PMPCB 9512
Co-fractionation Homo sapiens
27 TXN 7295
Co-fractionation Homo sapiens
28 KDELC2 143888
Co-fractionation Homo sapiens
29 CDH1 999
Proximity Label-MS Homo sapiens
30 MYC  
Affinity Capture-MS Homo sapiens
31 ZFHX3  
Affinity Capture-MS Homo sapiens
32 CCS 9973
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
33 TPI1 7167
Co-fractionation Homo sapiens
34 GOT1 2805
Co-fractionation Homo sapiens
35 DPH5 51611
Co-fractionation Homo sapiens
36 ACTB 60
Co-fractionation Homo sapiens
37 APBA1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
38 SCRN2 90507
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 SLC31A1 1317
Affinity Capture-Western Homo sapiens
40 GMPS 8833
Co-fractionation Homo sapiens
41 ATOX1 475
Co-fractionation Homo sapiens
42 FDX1L  
Co-fractionation Homo sapiens
43 Sod1 20655
Affinity Capture-MS Mus musculus
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