Experiment description of studies that identified NUSAP1 in sEVs

1 Experiment ID 126 MISEV standards ✘ Biophysical techniques ✔ GAPDH Enriched markers ✘ Negative markers ✘ Particle analysis Identified molecule protein Identification method Mass spectrometry [LTQ-FT Ultra] PubMed ID Unpublished / Not applicable Organism Homo sapiens Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim" Journal name Mesenchymal Stem Cell Therapy Publication year 2011 Sample Mesenchymal stem cells Sample name huES9.E1 Isolation/purification methods HPLC Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Antobody array Mass spectrometry

2 Experiment ID 834 MISEV standards ✔ EM Biophysical techniques ✔ LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

3 Experiment ID 835 MISEV standards ✔ EM Biophysical techniques ✔ CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101 Enriched markers ✔ CANX Negative markers ✔ NTA Particle analysis Identified molecule Protein Identification method Mass spectrometry PubMed ID 35333565     Organism Homo sapiens Experiment description LAMP2A regulates the loading of proteins into exosomes Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P" Journal name Sci Adv Publication year 2022 Sample Retinal pigment epithelial cells Sample name ARPE-19 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Flotation density - Molecules identified in the study Protein Methods used in the study Western blotting Mass spectrometry

Protein-protein interactions for NUSAP1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 CDC14B 8555 Proximity Label-MS Homo sapiens 2 HECTD1 25831 Affinity Capture-MS Homo sapiens 3 SOX2   Affinity Capture-MS Homo sapiens 4 MCM3 4172 Co-fractionation Homo sapiens 5 MECP2 4204 Affinity Capture-MS Homo sapiens 6 RPL23A 6147 Affinity Capture-MS Homo sapiens 7 TMPO 7112 Affinity Capture-MS Homo sapiens 8 AP2B1 163 Co-fractionation Homo sapiens 9 FASN 2194 Negative Genetic Homo sapiens 10 S100P 6286 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 11 CSNK2B 1460 Affinity Capture-MS Homo sapiens 12 C5orf30   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 13 HIST1H3A 8350 Proximity Label-MS Homo sapiens 14 NR4A1   Affinity Capture-MS Homo sapiens 15 UGP2 7360 Co-fractionation Homo sapiens 16 UNC119B 84747 Affinity Capture-MS Homo sapiens 17 KIF14 9928 Affinity Capture-MS Homo sapiens 18 HIST1H4A 8359 Affinity Capture-MS Homo sapiens 19 USP15 9958 Co-fractionation Homo sapiens 20 IPO7 10527 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 21 KLF16   Proximity Label-MS Homo sapiens 22 HEXIM1 10614 Affinity Capture-MS Homo sapiens 23 CEBPA   Protein-peptide Homo sapiens 24 POP1 10940 Affinity Capture-MS Homo sapiens 25 Nup188   Affinity Capture-MS Mus musculus 26 KPNB1 3837 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 27 KLF9 687 Proximity Label-MS Homo sapiens 28 GAPDHS 26330 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 Kif2c 73804 Two-hybrid Mus musculus 30 UBE4B 10277 Co-fractionation Homo sapiens 31 SLC9A3R2 9351 Co-fractionation Homo sapiens 32 CTTN 2017 Affinity Capture-MS Homo sapiens 33 ELAVL1 1994 Affinity Capture-RNA Homo sapiens 34 PSMD10 5716 Co-fractionation Homo sapiens 35 CLUH 23277 Co-fractionation Homo sapiens 36 CCNF   Affinity Capture-Western Homo sapiens 37 PPP1R10   Affinity Capture-MS Homo sapiens 38 RANBP2 5903 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 39 HPDL 84842 Co-fractionation Homo sapiens 40 APP 351 Reconstituted Complex Homo sapiens Reconstituted Complex Homo sapiens 41 PIP4K2A 5305 Affinity Capture-MS Homo sapiens 42 USP36   Affinity Capture-MS Homo sapiens 43 RCL1 10171 Affinity Capture-MS Homo sapiens 44 KHDRBS2   Affinity Capture-MS Homo sapiens 45 PLOD2 5352 Co-fractionation Homo sapiens 46 PPP1R12A 4659 Co-fractionation Homo sapiens 47 GSTA2 2939 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 48 LRPAP1 4043 Affinity Capture-MS Homo sapiens 49 MYC   Affinity Capture-MS Homo sapiens 50 PRDX3 10935 Co-fractionation Homo sapiens 51 CEP70   Two-hybrid Homo sapiens 52 RAN 5901 Affinity Capture-MS Homo sapiens 53 CHAF1A   Co-fractionation Homo sapiens 54 PTPN12 5782 Co-fractionation Homo sapiens 55 B2M 567 Affinity Capture-MS Homo sapiens 56 U2AF2 11338 Affinity Capture-MS Homo sapiens 57 TAF2   Affinity Capture-MS Homo sapiens 58 Ranbp2   Affinity Capture-MS Mus musculus 59 HP1BP3 50809 Affinity Capture-MS Homo sapiens 60 DDX42 11325 Affinity Capture-MS Homo sapiens 61 KIFC1 3833 Affinity Capture-MS Homo sapiens 62 CPSF7 79869 Affinity Capture-MS Homo sapiens 63 CSNK1E 1454 Affinity Capture-MS Homo sapiens 64 RANBP1 5902 Affinity Capture-MS Homo sapiens 65 CST6   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 66 RPS6 6194 Proximity Label-MS Homo sapiens 67 DDRGK1 65992 Affinity Capture-MS Homo sapiens 68 METTL17   Affinity Capture-MS Homo sapiens 69 HIST1H2BG 8339 Proximity Label-MS Homo sapiens 70 RRP1B 23076 Affinity Capture-MS Homo sapiens 71 Ube2i   Affinity Capture-MS Mus musculus 72 RPS24 6229 Proximity Label-MS Homo sapiens 73 MYCN   Affinity Capture-MS Homo sapiens 74 TFAM 7019 Affinity Capture-MS Homo sapiens 75 DDX56   Affinity Capture-MS Homo sapiens 76 DAXX   Affinity Capture-MS Homo sapiens 77 RCC1 1104 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 78 NUP153 9972 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 79 OGT 8473 Reconstituted Complex Homo sapiens 80 KIF20A 10112 Affinity Capture-MS Homo sapiens View the network

Pathways in which NUSAP1 is involved

No pathways found