Gene description for SPEN
Gene name spen family transcriptional repressor
Gene symbol SPEN
Other names/aliases HIAA0929
MINT
RBM15C
SHARP
Species Homo sapiens
 Database cross references - SPEN
ExoCarta ExoCarta_23013
Vesiclepedia VP_23013
Entrez Gene 23013
HGNC 17575
MIM 613484
UniProt Q96T58  
 SPEN identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
 Gene ontology annotations for SPEN
Molecular Function
    DNA binding GO:0003677 IEA
    transcription corepressor activity GO:0003714 IDA
    RNA binding GO:0003723 HDA
    mRNA binding GO:0003729 IBA
    protein binding GO:0005515 IPI
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    Notch signaling pathway GO:0007219 IEA
    regulatory ncRNA-mediated heterochromatin formation GO:0031048 IMP
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of neurogenesis GO:0050769 IMP
    random inactivation of X chromosome GO:0060816 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    transcription repressor complex GO:0017053 IDA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SPEN in exosomes
1
Experiment ID 207
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
MISEV standards
EM
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
MISEV standards
Biophysical techniques
TSG101|HSP70|FLOT1
Enriched markers
VDAC
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
5
Experiment ID 235
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 63
MISEV standards
Biophysical techniques
AQP2
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for SPEN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CAMKV 79012
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 ARL6IP5 10550
Cross-Linking-MS (XL-MS) Homo sapiens
3 SP1  
Co-fractionation Homo sapiens
4 HIST1H2BG 8339
Proximity Label-MS Homo sapiens
5 DHX8 1659
Proximity Label-MS Homo sapiens
6 HSP90AB1 3326
Cross-Linking-MS (XL-MS) Homo sapiens
7 PIR 8544
Co-fractionation Homo sapiens
8 CFL1 1072
Cross-Linking-MS (XL-MS) Homo sapiens
9 PCBP3 54039
Co-fractionation Homo sapiens
10 DPPA4  
Affinity Capture-MS Homo sapiens
11 SRA1 10011
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
12 NCOR1  
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
13 FERMT3 83706
Affinity Capture-MS Homo sapiens
14 SULF2 55959
Affinity Capture-MS Homo sapiens
15 PRDX3 10935
Co-fractionation Homo sapiens
16 UBE2E2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
17 KLF8  
Proximity Label-MS Homo sapiens
18 NFKBIL1  
Affinity Capture-MS Homo sapiens
19 PPARD  
Affinity Capture-Western Homo sapiens
20 PRC1 9055
Affinity Capture-MS Homo sapiens
21 GUSBP5  
Affinity Capture-MS Homo sapiens
22 OBSL1 23363
Affinity Capture-MS Homo sapiens
23 EED  
Affinity Capture-MS Homo sapiens
24 MECP2 4204
Affinity Capture-MS Homo sapiens
25 ASF1A 25842
Proximity Label-MS Homo sapiens
26 RBPJ 3516
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
27 NOTCH2 4853
Co-fractionation Homo sapiens
28 RUNX1  
Protein-peptide Homo sapiens
29 TRIML2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
31 RPL4 6124
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
32 CTBP1 1487
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
33 NCOR2  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
34 HRSP12 10247
Two-hybrid Homo sapiens
35 WRAP73 49856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 NFIX 4784
Proximity Label-MS Homo sapiens
37 SRSF6 6431
Affinity Capture-MS Homo sapiens
38 CBX3 11335
Proximity Label-MS Homo sapiens
39 LOC102724023  
Cross-Linking-MS (XL-MS) Homo sapiens
40 SERTAD1  
Two-hybrid Homo sapiens
41 TTN 7273
Co-fractionation Homo sapiens
42 RAD18  
Affinity Capture-MS Homo sapiens
43 ARRDC3 57561
Affinity Capture-MS Homo sapiens
44 T  
Proximity Label-MS Homo sapiens
45 CEBPA  
Proximity Label-MS Homo sapiens
46 DAXX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CT45A5  
Affinity Capture-MS Homo sapiens
48 BCLAF1 9774
Affinity Capture-MS Homo sapiens
49 ATXN1 6310
Two-hybrid Homo sapiens
50 RNF40 9810
Affinity Capture-MS Homo sapiens
51 HIST1H3A 8350
Proximity Label-MS Homo sapiens
52 ABT1 29777
Affinity Capture-MS Homo sapiens
53 CCNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 BAG2 9532
Affinity Capture-MS Homo sapiens
55 SIRT7  
Affinity Capture-MS Homo sapiens
56 UBE2L6 9246
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
57 NR3C1 2908
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
58 EGFR 1956
Affinity Capture-MS Homo sapiens
59 ANKRD36B  
Affinity Capture-MS Homo sapiens
60 SCYL2 55681
Co-fractionation Homo sapiens
61 HNF4A  
Proximity Label-MS Homo sapiens
62 RUNX1T1  
Protein-peptide Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
63 PIP4K2A 5305
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 RPA3 6119
Proximity Label-MS Homo sapiens
65 ZC3H18  
Affinity Capture-MS Homo sapiens
66 HSPA1A 3303
Affinity Capture-MS Homo sapiens
67 BMI1  
Affinity Capture-MS Homo sapiens
68 FHL2 2274
Affinity Capture-MS Homo sapiens
69 BAG4  
Affinity Capture-MS Homo sapiens
70 RBBP8  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
71 NUP54 53371
Co-fractionation Homo sapiens
72 USP37  
Proximity Label-MS Homo sapiens
73 HSPA8 3312
Affinity Capture-MS Homo sapiens
74 MEN1 4221
Affinity Capture-MS Homo sapiens
75 PLCD3 113026
Affinity Capture-MS Homo sapiens
76 HDAC1 3065
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
77 CHD4 1108
Affinity Capture-MS Homo sapiens
78 KCNE3  
Affinity Capture-MS Homo sapiens
79 MKI67  
Affinity Capture-MS Homo sapiens
80 BPGM 669
Co-fractionation Homo sapiens
81 NUP50 10762
Proximity Label-MS Homo sapiens
82 Srsf1 110809
Affinity Capture-MS Mus musculus
83 TRIM37  
Proximity Label-MS Homo sapiens
84 CUL7 9820
Affinity Capture-MS Homo sapiens
85 CCT8L2  
Affinity Capture-MS Homo sapiens
86 HSPA2 3306
Affinity Capture-MS Homo sapiens
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