Gene description for CYP51A1
Gene name cytochrome P450, family 51, subfamily A, polypeptide 1
Gene symbol CYP51A1
Other names/aliases CP51
CYP51
CYPL1
LDM
P450-14DM
P450L1
Species Homo sapiens
 Database cross references - CYP51A1
ExoCarta ExoCarta_1595
Vesiclepedia VP_1595
Entrez Gene 1595
HGNC 2649
MIM 601637
UniProt Q16850  
 CYP51A1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for CYP51A1
Molecular Function
    iron ion binding GO:0005506 IEA
    sterol 14-demethylase activity GO:0008398 IDA
    oxidoreductase activity GO:0016491 IBA
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen GO:0016712 TAS
    heme binding GO:0020037 IDA
Biological Process
    steroid biosynthetic process GO:0006694 IDA
    cholesterol biosynthetic process GO:0006695 TAS
    sterol metabolic process GO:0016125 TAS
    cholesterol biosynthetic process via 24,25-dihydrolanosterol GO:0033488 IBA
    negative regulation of protein catabolic process GO:0042177 ISS
    negative regulation of protein secretion GO:0050709 ISS
    negative regulation of amyloid-beta clearance GO:1900222 ISS
Subcellular Localization
    endoplasmic reticulum membrane GO:0005789 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified CYP51A1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for CYP51A1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 OR2M7  
Affinity Capture-MS Homo sapiens
2 HSD17B11 51170
Proximity Label-MS Homo sapiens
3 OR10H1  
Affinity Capture-MS Homo sapiens
4 LGR4 55366
Affinity Capture-MS Homo sapiens
5 RXFP1  
Affinity Capture-MS Homo sapiens
6 OR10H2  
Affinity Capture-MS Homo sapiens
7 METTL7A 25840
Proximity Label-MS Homo sapiens
8 TRIAP1  
Co-fractionation Homo sapiens
9 PCDHGB5 56101
Affinity Capture-MS Homo sapiens
10 SRPRB 58477
Affinity Capture-MS Homo sapiens
11 PCDHGB4 8641
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 HRAS 3265
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 C9orf78 51759
Affinity Capture-MS Homo sapiens
14 TMEM9B 56674
Affinity Capture-MS Homo sapiens
15 DNAJC25 548645
Proximity Label-MS Homo sapiens
16 TXNDC12 51060
Co-fractionation Homo sapiens
17 RTN4 57142
Affinity Capture-MS Homo sapiens
18 PTCHD3  
Affinity Capture-MS Homo sapiens
19 DEGS1  
Affinity Capture-MS Homo sapiens
20 MS4A15  
Affinity Capture-MS Homo sapiens
21 HTR3E  
Affinity Capture-MS Homo sapiens
22 PRDX3 10935
Co-fractionation Homo sapiens
23 KIF23 9493
Affinity Capture-MS Homo sapiens
24 FAM57A  
Affinity Capture-MS Homo sapiens
25 COPB2 9276
Affinity Capture-MS Homo sapiens
26 CYP19A1  
Affinity Capture-MS Homo sapiens
27 YES1 7525
Affinity Capture-MS Homo sapiens
28 TREML2 79865
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SLC31A1 1317
Affinity Capture-MS Homo sapiens
30 PIGH  
Affinity Capture-MS Homo sapiens
31 C3orf52  
Affinity Capture-MS Homo sapiens
32 TTYH1  
Affinity Capture-MS Homo sapiens
33 CCDC47 57003
Affinity Capture-MS Homo sapiens
34 MECP2 4204
Affinity Capture-MS Homo sapiens
35 SNX14  
Affinity Capture-MS Homo sapiens
36 EMD 2010
Proximity Label-MS Homo sapiens
37 PARK2  
Affinity Capture-MS Homo sapiens
38 SCAP  
Affinity Capture-MS Homo sapiens
39 TMEM150A  
Affinity Capture-MS Homo sapiens
40 ZDHHC12  
Affinity Capture-MS Homo sapiens
41 ABHD11 83451
Co-fractionation Homo sapiens
42 PCDHGC4  
Affinity Capture-MS Homo sapiens
43 CCDC107  
Affinity Capture-MS Homo sapiens
44 CCNY 219771
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 PTPRO 5800
Affinity Capture-MS Homo sapiens
46 SEC61B 10952
Proximity Label-MS Homo sapiens
47 CLRN2  
Affinity Capture-MS Homo sapiens
48 EIF4A2 1974
Affinity Capture-MS Homo sapiens
49 KCNS3 3790
Affinity Capture-MS Homo sapiens
50 ZNF420  
Two-hybrid Homo sapiens
51 HADHA 3030
Co-fractionation Homo sapiens
52 RPN2 6185
Proximity Label-MS Homo sapiens
53 HMGCLL1  
Co-fractionation Homo sapiens
54 CANX 821
Affinity Capture-MS Homo sapiens
55 POTEI 653269
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 TM4SF18  
Affinity Capture-MS Homo sapiens
57 ADCY9 115
Affinity Capture-MS Homo sapiens
58 MCOLN3  
Affinity Capture-MS Homo sapiens
59 CALCOCO1 57658
Affinity Capture-MS Homo sapiens
60 IL22RA1 58985
Affinity Capture-MS Homo sapiens
61 HSD3B7 80270
Proximity Label-MS Homo sapiens
62 ACTBL2 345651
Affinity Capture-MS Homo sapiens
63 NR3C1 2908
Affinity Capture-MS Homo sapiens
64 CCP110  
Affinity Capture-MS Homo sapiens
65 PCDHA3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 FTH1 2495
Co-fractionation Homo sapiens
67 SSTR1 6751
Affinity Capture-MS Homo sapiens
68 RPA3 6119
Proximity Label-MS Homo sapiens
69 ATP2A1 487
Proximity Label-MS Homo sapiens
70 MARCH4  
Affinity Capture-MS Homo sapiens
71 HSPA4 3308
Co-fractionation Homo sapiens
72 TGFA  
Affinity Capture-MS Homo sapiens
73 C9orf91 203197
Affinity Capture-MS Homo sapiens
74 KCNK16  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 HUWE1 10075
Affinity Capture-MS Homo sapiens
76 AQP9  
Affinity Capture-MS Homo sapiens
77 KCNA2  
Affinity Capture-MS Homo sapiens
78 HLA-DMB 3109
Affinity Capture-MS Homo sapiens
79 PGRMC1 10857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 TSPAN17 26262
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 ATG101  
Affinity Capture-MS Homo sapiens
82 PPP1R9B 84687
Affinity Capture-MS Homo sapiens
83 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
84 PTGER3  
Affinity Capture-MS Homo sapiens
85 EVA1C  
Affinity Capture-MS Homo sapiens
86 CGRRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 RAB7A 7879
Proximity Label-MS Homo sapiens
88 FAM189A2  
Affinity Capture-MS Homo sapiens
89 SERBP1 26135
Affinity Capture-MS Homo sapiens
90 EVA1B 55194
Affinity Capture-MS Homo sapiens
91 C9orf72  
Affinity Capture-MS Homo sapiens
92 DERL1 79139
Proximity Label-MS Homo sapiens
93 SPINT2 10653
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 INSRR 3645
Affinity Capture-MS Homo sapiens
95 PCDHB3  
Affinity Capture-MS Homo sapiens
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