Gene description for OGDH
Gene name oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Gene symbol OGDH
Other names/aliases AKGDH
E1k
OGDC
Species Homo sapiens
 Database cross references - OGDH
ExoCarta ExoCarta_4967
Vesiclepedia VP_4967
Entrez Gene 4967
HGNC 8124
MIM 613022
UniProt Q02218  
 OGDH identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Breast cancer cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Embryonic kidney cells 34108659    
Foreskin fibroblasts 34108659    
Lymphoma cells 34108659    
Mammary cancer-associated fibroblasts 34108659    
Mesenchymal stem cells 36408942    
Monocytic leukemia cells 34108659    
Normal mammary epithelial cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic cancer cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pancreatic duct epithalial cells 34108659    
Pluripotent stem cells 34108659    
T lymphocytes 34108659    
 Gene ontology annotations for OGDH
Molecular Function
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 IBA
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 IDA
    oxoglutarate dehydrogenase (succinyl-transferring) activity GO:0004591 ISS
    protein binding GO:0005515 IPI
    thiamine pyrophosphate binding GO:0030976 IDA
    oxoglutarate dehydrogenase (NAD+) activity GO:0034602 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    generation of precursor metabolites and energy GO:0006091 ISS
    glycolytic process GO:0006096 IEA
    tricarboxylic acid cycle GO:0006099 IBA
    2-oxoglutarate metabolic process GO:0006103 IDA
    succinyl-CoA metabolic process GO:0006104 IDA
    cerebellar cortex development GO:0021695 IEA
    striatum development GO:0021756 IEA
    hippocampus development GO:0021766 IEA
    thalamus development GO:0021794 IEA
    pyramidal neuron development GO:0021860 IEA
    tangential migration from the subventricular zone to the olfactory bulb GO:0022028 IEA
    olfactory bulb mitral cell layer development GO:0061034 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    mitochondrial matrix GO:0005759 TAS
    mitochondrial membrane GO:0031966 ISS
    oxoglutarate dehydrogenase complex GO:0045252 IBA
    oxoglutarate dehydrogenase complex GO:0045252 IDA
 Experiment description of studies that identified OGDH in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MCF7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
6
Experiment ID 414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
7
Experiment ID 426
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 427
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Breast cancer cells
Sample name MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
10
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 419
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
13
Experiment ID 420
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Embryonic kidney cells
Sample name HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Foreskin fibroblasts
Sample name BJ
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
15
Experiment ID 417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Lymphoma cells
Sample name Raji
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
16
Experiment ID 411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Mammary cancer-associated fibroblasts
Sample name mCAF
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
17
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
18
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
19
Experiment ID 418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Monocytic leukemia cells
Sample name THP-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
20
Experiment ID 413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Normal mammary epithelial cells
Sample name MCF10A
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
21
Experiment ID 406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name BxPC3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
22
Experiment ID 415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
23
Experiment ID 434
MISEV standards
✘ Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
24
Experiment ID 435
MISEV standards
✘ Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic cancer cells
Sample name PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
25
Experiment ID 408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPDE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
26
Experiment ID 409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pancreatic duct epithalial cells
Sample name HPNE
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
27
Experiment ID 416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample Pluripotent stem cells
Sample name PSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
28
Experiment ID 410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 34108659    
Organism Homo sapiens
Experiment description Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors "Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name Nat Cell Biol
Publication year 2021
Sample T lymphocytes
Sample name Jurkat
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
mRNA
Methods used in the study RT-qPCR
Western blotting
Mass spectrometry
Flow cytometry
 Protein-protein interactions for OGDH
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 COX5A 9377
Co-fractionation Homo sapiens
2 CYC1 1537
Co-fractionation Homo sapiens
3 SLIRP 81892
Proximity Label-MS Homo sapiens
4 ISG15 9636
Affinity Capture-MS Homo sapiens
5 EMC2 9694
Co-fractionation Homo sapiens
6 NDUFS4 4724
Co-fractionation Homo sapiens
7 RAB1A 5861
Co-fractionation Homo sapiens
8 NDUFS1 4719
Co-fractionation Homo sapiens
9 USP13  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 CSNK1A1 1452
Co-fractionation Homo sapiens
11 USP5 8078
Co-fractionation Homo sapiens
12 SUCLG1 8802
Co-fractionation Homo sapiens
13 MTG2  
Proximity Label-MS Homo sapiens
14 NGRN  
Proximity Label-MS Homo sapiens
15 APOE 348
Co-fractionation Homo sapiens
16 MRRF  
Proximity Label-MS Homo sapiens
17 PDE6A  
Cross-Linking-MS (XL-MS) Homo sapiens
18 DNAJC15  
Proximity Label-MS Homo sapiens
19 NDUFS2 4720
Co-fractionation Homo sapiens
20 GFM2 84340
Proximity Label-MS Homo sapiens
21 CCDC90B  
Proximity Label-MS Homo sapiens
22 TOMM22 56993
Co-fractionation Homo sapiens
23 LRPPRC 10128
Proximity Label-MS Homo sapiens
24 KIAA1429 25962
Affinity Capture-MS Homo sapiens
25 TRUB2  
Proximity Label-MS Homo sapiens
26 MGST1 4257
Co-fractionation Homo sapiens
27 WDR76  
Affinity Capture-MS Homo sapiens
28 NDUFS3 4722
Co-fractionation Homo sapiens
29 CRYZ 1429
Proximity Label-MS Homo sapiens
30 PRDX3 10935
Co-fractionation Homo sapiens
31 PMPCA 23203
Proximity Label-MS Homo sapiens
32 HINT2 84681
Proximity Label-MS Homo sapiens
33 CYB5R1 51706
Co-fractionation Homo sapiens
34 MRPS36 92259
Affinity Capture-MS Homo sapiens
35 C8orf82  
Proximity Label-MS Homo sapiens
36 CPSF7 79869
Co-fractionation Homo sapiens
37 COA3 28958
Co-fractionation Homo sapiens
38 SLC25A12 8604
Proximity Label-MS Homo sapiens
39 C21orf33  
Proximity Label-MS Homo sapiens
40 MRPS26 64949
Proximity Label-MS Homo sapiens
41 SURF1  
Proximity Label-MS Homo sapiens
42 ATP5O 539
Co-fractionation Homo sapiens
43 EXD2  
Proximity Label-MS Homo sapiens
44 NDUFV1 4723
Co-fractionation Homo sapiens
45 PHB 5245
Co-fractionation Homo sapiens
46 DHODH 1723
Co-fractionation Homo sapiens
47 ATP5H 10476
Co-fractionation Homo sapiens
48 MECP2 4204
Affinity Capture-MS Homo sapiens
49 PMPCB 9512
Proximity Label-MS Homo sapiens
50 TACO1  
Proximity Label-MS Homo sapiens
51 FASTKD2  
Proximity Label-MS Homo sapiens
52 SERBP1 26135
Affinity Capture-MS Homo sapiens
53 NDUFA6  
Co-fractionation Homo sapiens
54 VWA8 23078
Proximity Label-MS Homo sapiens
55 UQCRQ 27089
Co-fractionation Homo sapiens
56 NDUFS5 4725
Co-fractionation Homo sapiens
57 MTERF3  
Proximity Label-MS Homo sapiens
58 KIF14 9928
Affinity Capture-MS Homo sapiens
59 MDH2 4191
Proximity Label-MS Homo sapiens
60 UNK  
Affinity Capture-RNA Homo sapiens
61 MRPL38  
Co-fractionation Homo sapiens
62 MRPL13  
Co-fractionation Homo sapiens
63 DLD 1738
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
64 ATP5A1 498
Co-fractionation Homo sapiens
65 SCFD1 23256
Co-fractionation Homo sapiens
66 C6orf203  
Proximity Label-MS Homo sapiens
67 TSR1 55720
Co-fractionation Homo sapiens
68 COX15 1355
Proximity Label-MS Homo sapiens
69 FASTKD5  
Proximity Label-MS Homo sapiens
70 PARK2  
Affinity Capture-MS Homo sapiens
71 STOML2 30968
Co-fractionation Homo sapiens
72 NDUFA8 4702
Co-fractionation Homo sapiens
73 TUFM 7284
Proximity Label-MS Homo sapiens
74 MTIF3  
Proximity Label-MS Homo sapiens
75 CERS2 29956
Affinity Capture-MS Homo sapiens
76 HSCB 150274
Proximity Label-MS Homo sapiens
77 SLC25A20 788
Co-fractionation Homo sapiens
78 MDM2  
Affinity Capture-MS Homo sapiens
79 RPA2 6118
Proximity Label-MS Homo sapiens
80 NTRK1 4914
Affinity Capture-MS Homo sapiens
81 PDHX 8050
Co-fractionation Homo sapiens
82 CCDC109B 55013
Proximity Label-MS Homo sapiens
83 C20orf24  
Co-fractionation Homo sapiens
84 EXOSC5 56915
Affinity Capture-MS Homo sapiens
85 TCAIM  
Affinity Capture-MS Homo sapiens
86 DNAJA1 3301
Co-fractionation Homo sapiens
87 MOV10 4343
Affinity Capture-RNA Homo sapiens
88 HUWE1 10075
Affinity Capture-MS Homo sapiens
89 GJB1 2705
Affinity Capture-MS Homo sapiens
90 UQCRC1 7384
Co-fractionation Homo sapiens
91 ACTB 60
Co-fractionation Homo sapiens
92 MCU 90550
Proximity Label-MS Homo sapiens
93 MTFMT  
Proximity Label-MS Homo sapiens
94 MRPL11 65003
Proximity Label-MS Homo sapiens
95 MTRF1L  
Proximity Label-MS Homo sapiens
96 AGPS 8540
Co-fractionation Homo sapiens
97 GNG5 2787
Co-fractionation Homo sapiens
98 VDAC2 7417
Co-fractionation Homo sapiens
99 NIPSNAP1 8508
Co-fractionation Homo sapiens
100 ATP5B 506
Co-fractionation Homo sapiens
101 MRPL41 64975
Co-fractionation Homo sapiens
102 ANLN 54443
Affinity Capture-MS Homo sapiens
103 OTC 5009
Proximity Label-MS Homo sapiens
104 PREB 10113
Co-fractionation Homo sapiens
105 HADHB 3032
Co-fractionation Homo sapiens
106 HADHA 3030
Co-fractionation Homo sapiens
107 MINOS1  
Co-fractionation Homo sapiens
108 AIFM1 9131
Co-fractionation Homo sapiens
109 MRPS12  
Proximity Label-MS Homo sapiens
110 C19orf52  
Proximity Label-MS Homo sapiens
111 MTIF2 4528
Proximity Label-MS Homo sapiens
112 CHCHD1  
Proximity Label-MS Homo sapiens
113 SLC25A51  
Proximity Label-MS Homo sapiens
114 LETM1 3954
Co-fractionation Homo sapiens
115 UQCRC2 7385
Co-fractionation Homo sapiens
116 EEF2 1938
Co-fractionation Homo sapiens
117 COX7A2 1347
Co-fractionation Homo sapiens
118 RMND1 55005
Proximity Label-MS Homo sapiens
119 HSD17B10 3028
Two-hybrid Homo sapiens
120 ATP1B3 483
Co-fractionation Homo sapiens
121 ADAM7  
Cross-Linking-MS (XL-MS) Homo sapiens
122 NXF1 10482
Affinity Capture-RNA Homo sapiens
123 MRPL15 29088
Co-fractionation Homo sapiens
124 AURKB 9212
Affinity Capture-MS Homo sapiens
125 C12orf65  
Proximity Label-MS Homo sapiens
126 MTG1  
Proximity Label-MS Homo sapiens
127 RPUSD4 84881
Proximity Label-MS Homo sapiens
128 RPUSD3  
Proximity Label-MS Homo sapiens
129 RPA3 6119
Proximity Label-MS Homo sapiens
130 DHX30 22907
Proximity Label-MS Homo sapiens
131 ATP6V1A 523
Co-fractionation Homo sapiens
132 DLST 1743
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
133 ACAD9 28976
Proximity Label-MS Homo sapiens
134 ENO1 2023
Affinity Capture-RNA Homo sapiens
135 VDAC1 7416
Co-fractionation Homo sapiens
136 KRAS 3845
Negative Genetic Homo sapiens
137 COX2 4513
Co-fractionation Homo sapiens
138 HSPA9 3313
Proximity Label-MS Homo sapiens
139 DLAT 1737
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
140 NDUFC2 4718
Co-fractionation Homo sapiens
141 METTL15  
Proximity Label-MS Homo sapiens
142 MCUR1 63933
Proximity Label-MS Homo sapiens
143 SCCPDH 51097
Co-fractionation Homo sapiens
144 MTRF1  
Proximity Label-MS Homo sapiens
145 PLSCR1 5359
Two-hybrid Homo sapiens
146 HECTD1 25831
Affinity Capture-MS Homo sapiens
147 CLPP 8192
Proximity Label-MS Homo sapiens
148 CS 1431
Proximity Label-MS Homo sapiens
149 C17orf80 55028
Proximity Label-MS Homo sapiens
150 PHB2 11331
Co-fractionation Homo sapiens
151 TSFM 10102
Proximity Label-MS Homo sapiens
152 AUH 549
Proximity Label-MS Homo sapiens
153 MRPS2 51116
Co-fractionation Homo sapiens
154 TBRG4 9238
Proximity Label-MS Homo sapiens
155 DNAJC19 131118
Proximity Label-MS Homo sapiens
156 TP53 7157
Affinity Capture-MS Homo sapiens
157 ICT1 3396
Proximity Label-MS Homo sapiens
158 SUCLG2 8801
Co-fractionation Homo sapiens
159 LONP1 9361
Proximity Label-MS Homo sapiens
160 HDAC6 10013
Affinity Capture-MS Homo sapiens
161 ZNF782  
Co-fractionation Homo sapiens
162 PDHA1 5160
Co-fractionation Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
163 CAPZA2 830
Affinity Capture-MS Homo sapiens
164 FKBP9 11328
Co-fractionation Homo sapiens
165 PDHB 5162
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
166 Hdac6  
Affinity Capture-MS Mus musculus
167 OGDHL  
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
168 SSBP1 6742
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
169 SFXN1 94081
Co-fractionation Homo sapiens
170 TMEM70  
Proximity Label-MS Homo sapiens
171 FASTKD3  
Proximity Label-MS Homo sapiens
172 NDUFS8 4728
Co-fractionation Homo sapiens
173 GFM1 85476
Proximity Label-MS Homo sapiens
174 TEFM  
Proximity Label-MS Homo sapiens
175 TOMM5  
Co-fractionation Homo sapiens
176 RAB7A 7879
Co-fractionation Homo sapiens
177 PCCB 5096
Co-fractionation Homo sapiens
178 CYB5R3 1727
Co-fractionation Homo sapiens
179 GRSF1 2926
Proximity Label-MS Homo sapiens
180 C1QBP 708
Proximity Label-MS Homo sapiens
181 CEBPB  
Co-fractionation Homo sapiens
182 ATP5C1 509
Co-fractionation Homo sapiens
183 METTL17  
Proximity Label-MS Homo sapiens
184 ATXN3 4287
Affinity Capture-MS Homo sapiens
185 TFAM 7019
Proximity Label-MS Homo sapiens
186 DDX28  
Proximity Label-MS Homo sapiens
187 MTCH2 23788
Co-fractionation Homo sapiens
188 NDUFB4 4710
Co-fractionation Homo sapiens
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