Experiment description of studies that identified PEG10 in sEVs

1 Experiment ID 226 MISEV standards ✔ EM Biophysical techniques ✔ GAPDH|CD9|FLOT1 Enriched markers ✔ LMNA|H2AFX|ATP5A1|TOMM20 Negative markers ✘ Particle analysis Identified molecule mRNA Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa) PubMed ID 26027894     Organism Homo sapiens Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting" Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar" Journal name Journal of Extracellular Vesicles Publication year 2015 Sample Endothelial cells Sample name HMEC-1 Isolation/purification methods Differential ultracentrifugation Sucrose density gradient Flotation density 1.10 g/mL Molecules identified in the study miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting

2 Experiment ID 234 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-C3 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

3 Experiment ID 235 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocellular carcinoma cells Sample name HKCI-8 Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RT-PCR RNA Sequencing

4 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for PEG10

Protein Interactor ExoCarta ID Identification method PubMed Species 1 SIAH1   Reconstituted Complex Homo sapiens Two-hybrid Homo sapiens Two-hybrid Homo sapiens 2 FAM127B   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 3 SRGAP1 57522 Affinity Capture-MS Homo sapiens 4 UBE2H 7328 Affinity Capture-MS Homo sapiens 5 SIAH2 6478 Two-hybrid Homo sapiens Reconstituted Complex Homo sapiens 6 Rbm14   Two-hybrid Mus musculus 7 CEP290   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 8 TMPO 7112 Affinity Capture-MS Homo sapiens 9 CCDC88A 55704 Affinity Capture-MS Homo sapiens 10 RNF181   Two-hybrid Homo sapiens 11 IRF4   Affinity Capture-MS Homo sapiens 12 SMCHD1 23347 Two-hybrid Homo sapiens 13 MAD2L2 10459 Proximity Label-MS Homo sapiens Affinity Capture-MS Homo sapiens 14 RBM14 10432 Affinity Capture-Western Homo sapiens 15 LNX2   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 16 NXF1 10482 Two-hybrid Homo sapiens 17 RBM25 58517 Affinity Capture-MS Homo sapiens 18 ATXN2 6311 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 19 PXMP2   Proximity Label-MS Homo sapiens 20 TCAF1 9747 Affinity Capture-MS Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 21 TOLLIP 54472 Affinity Capture-MS Homo sapiens Reconstituted Complex Homo sapiens 22 MEN1 4221 Affinity Capture-MS Homo sapiens 23 SFPQ 6421 Affinity Capture-MS Homo sapiens 24 SNRPA 6626 Two-hybrid Homo sapiens 25 MIB1 57534 Proximity Label-MS Homo sapiens 26 TSG101 7251 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens 27 CUL3 8452 Affinity Capture-MS Homo sapiens 28 NPL 80896 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 29 USP9X 8239 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 30 COMT 1312 Affinity Capture-MS Homo sapiens 31 Shoc2   Affinity Capture-MS Mus musculus 32 ATXN2L 11273 Affinity Capture-MS Homo sapiens 33 ATXN10 25814 Affinity Capture-Western Homo sapiens Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 34 ACBD5 91452 Proximity Label-MS Homo sapiens 35 RABL6 55684 Affinity Capture-MS Homo sapiens 36 HNRNPUL1 11100 Co-fractionation Homo sapiens 37 Ywhaz 22631 Two-hybrid Mus musculus 38 HMGCS1 3157 Co-fractionation Homo sapiens 39 NONO 4841 Affinity Capture-Western Homo sapiens 40 PLEKHA4 57664 Affinity Capture-MS Homo sapiens 41 COL1A1 1277 Affinity Capture-MS Homo sapiens 42 AP3D1 8943 Affinity Capture-MS Homo sapiens 43 MAP6 4135 Affinity Capture-Western Homo sapiens Affinity Capture-MS Homo sapiens 44 RBFOX2 23543 Two-hybrid Homo sapiens 45 OTUD1 220213 Affinity Capture-MS Homo sapiens 46 MAPT   FRET Homo sapiens 47 PSPC1 55269 Affinity Capture-MS Homo sapiens 48 BAG1 573 Co-fractionation Homo sapiens 49 GATA3   Affinity Capture-MS Homo sapiens 50 Ywhah 22629 Two-hybrid Mus musculus 51 Sap18   Two-hybrid Mus musculus 52 UPF1 5976 Affinity Capture-MS Homo sapiens 53 DNAJC7 7266 Proximity Label-MS Homo sapiens 54 IFI16 3428 Affinity Capture-MS Homo sapiens 55 Ube2i   Two-hybrid Mus musculus 56 RAB3IP   Two-hybrid Homo sapiens 57 IQCB1   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 58 EXOSC5 56915 Two-hybrid Homo sapiens 59 ACTB 60 Affinity Capture-MS Homo sapiens 60 LDOC1   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 61 SNX12 29934 Affinity Capture-MS Homo sapiens 62 SNW1 22938 Two-hybrid Homo sapiens 63 NPM1 4869 Proximity Label-MS Homo sapiens 64 ZNF512B   Two-hybrid Homo sapiens 65 POU5F1   Affinity Capture-MS Homo sapiens 66 Rpl35 66489 Affinity Capture-MS Mus musculus 67 CALCOCO2   Two-hybrid Homo sapiens 68 RC3H1 149041 Affinity Capture-MS Homo sapiens 69 FAM127A   Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 70 NANOG   Affinity Capture-MS Homo sapiens 71 ABCE1 6059 Affinity Capture-MS Homo sapiens 72 HNRNPH1 3187 Co-fractionation Homo sapiens 73 Tipin   Affinity Capture-MS Mus musculus 74 Ywhag 22628 Two-hybrid Mus musculus 75 FAM127C   Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens View the network

Pathways in which PEG10 is involved

No pathways found