Gene description for KRT79
Gene name keratin 79, type II
Gene symbol KRT79
Other names/aliases K6L
KRT6L
Species Homo sapiens
 Database cross references - KRT79
ExoCarta ExoCarta_338785
Vesiclepedia VP_338785
Entrez Gene 338785
HGNC 28930
MIM 611160
UniProt Q5XKE5  
 KRT79 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells 19837982    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells Unpublished / Not applicable
Neuroblastoma cells 25944692    
Prostate cancer cells 22723089    
Saliva 19199708    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for KRT79
Molecular Function
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
    structural constituent of skin epidermis GO:0030280 IBA
Biological Process
    keratinization GO:0031424 IBA
    intermediate filament organization GO:0045109 IBA
Subcellular Localization
    cytosol GO:0005829 TAS
    keratin filament GO:0045095 IBA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified KRT79 in sEVs
1
Experiment ID 21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry   
PubMed ID 19837982    
Organism Homo sapiens
Experiment description Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors "Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name MCP
Publication year 2009
Sample Colorectal cancer cells
Sample name LIM1215
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.10-1.12 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [Orbitrap]
Western blotting
2
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
6
Experiment ID 224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
7
Experiment ID 146
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors "Hosseini-Beheshti E, Guns ES."
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name RWPE - Rep 2
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
8
Experiment ID 66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19199708    
Organism Homo sapiens
Experiment description Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors "Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name JPR
Publication year 2009
Sample Saliva
Sample name Saliva
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
9
Experiment ID 191
MISEV standards
✘ Biophysical techniques
✔
Alix|CD81|CD9
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for KRT79
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
2 BAG3 9531
Affinity Capture-Luminescence Homo sapiens
3 KRT18 3875
Two-hybrid Homo sapiens
4 KRT84 3890
Cross-Linking-MS (XL-MS) Homo sapiens
5 BTRC 8945
Affinity Capture-MS Homo sapiens
6 KRT27 342574
Two-hybrid Homo sapiens
7 LGR4 55366
Affinity Capture-MS Homo sapiens
8 KRT36 8689
Two-hybrid Homo sapiens
9 KRT25 147183
Two-hybrid Homo sapiens
10 KRT33B 3884
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 CBL 867
Affinity Capture-MS Homo sapiens
12 INPPL1 3636
Affinity Capture-MS Homo sapiens
13 KRT6A 3853
Cross-Linking-MS (XL-MS) Homo sapiens
14 KRT35 3886
Two-hybrid Homo sapiens
15 CRK 1398
Affinity Capture-MS Homo sapiens
16 KRT34  
Two-hybrid Homo sapiens
17 PDHA1 5160
Proximity Label-MS Homo sapiens
18 EPS15 2060
Affinity Capture-MS Homo sapiens
19 NHLRC2 374354
Affinity Capture-MS Homo sapiens
20 KRT28 162605
Two-hybrid Homo sapiens
21 HNRNPA1 3178
Affinity Capture-MS Homo sapiens
22 SIRT1  
Affinity Capture-MS Homo sapiens
23 Cep152  
Affinity Capture-MS Mus musculus
24 TRIM54  
Two-hybrid Homo sapiens
25 TFCP2 7024
Affinity Capture-MS Homo sapiens
26 AP2M1 1173
Affinity Capture-MS Homo sapiens
27 KRT15 3866
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
28 KRT24 192666
Two-hybrid Homo sapiens
29 UBASH3B 84959
Affinity Capture-MS Homo sapiens
30 KRT20 54474
Cross-Linking-MS (XL-MS) Homo sapiens
31 KRT38 8687
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
32 Dctn3  
Affinity Capture-MS Mus musculus
33 EVI5L  
Two-hybrid Homo sapiens
34 KRT75 9119
Cross-Linking-MS (XL-MS) Homo sapiens
35 KRT13 3860
Two-hybrid Homo sapiens
36 KRT14 3861
Two-hybrid Homo sapiens
37 PIK3R2 5296
Affinity Capture-MS Homo sapiens
38 USHBP1  
Two-hybrid Homo sapiens
39 SYCE3  
Two-hybrid Homo sapiens
40 KRT19 3880
Two-hybrid Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
41 KRT5 3852
Cross-Linking-MS (XL-MS) Homo sapiens
42 YAP1 10413
Affinity Capture-MS Homo sapiens
43 KRT31 3881
Two-hybrid Homo sapiens
44 KRT16 3868
Two-hybrid Homo sapiens
45 KIF2C 11004
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which KRT79 is involved
PathwayEvidenceSource
Developmental Biology TAS Reactome
Formation of the cornified envelope TAS Reactome
Keratinization TAS Reactome





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