Gene description for NFE2L2
Gene name nuclear factor, erythroid 2-like 2
Gene symbol NFE2L2
Other names/aliases NRF2
Species Homo sapiens
 Database cross references - NFE2L2
ExoCarta ExoCarta_4780
Entrez Gene 4780
HGNC 7782
MIM 600492
UniProt Q16236  
 NFE2L2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for NFE2L2
Molecular Function
    RNA polymerase II activating transcription factor binding GO:0001102 IPI
    transcription cofactor binding GO:0001221 IEA
    transcription regulatory region DNA binding GO:0044212 TAS
    RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription GO:0001205 IEA
    protein domain specific binding GO:0019904 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 IDA
    DNA binding GO:0003677 IDA
    RNA polymerase II distal enhancer sequence-specific DNA binding GO:0000980 IEA
    transcription regulatory region sequence-specific DNA binding GO:0000976 TAS
    protein binding GO:0005515 IPI
Biological Process
    regulation of removal of superoxide radicals GO:2000121 IEA
    cellular response to tumor necrosis factor GO:0071356 IMP
    protein ubiquitination GO:0016567 IDA
    cellular response to hydrogen peroxide GO:0070301 IMP
    PERK-mediated unfolded protein response GO:0036499 TAS
    negative regulation of endothelial cell apoptotic process GO:2000352 IMP
    positive regulation of reactive oxygen species metabolic process GO:2000379 IEA
    cellular response to laminar fluid shear stress GO:0071499 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IDA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway GO:1902176 IMP
    positive regulation of gene expression GO:0010628 IGI
    transcription from RNA polymerase II promoter GO:0006366 TAS
    positive regulation of blood coagulation GO:0030194 IEA
    proteasomal ubiquitin-independent protein catabolic process GO:0010499 IDA
    endoplasmic reticulum unfolded protein response GO:0030968 ISS
    cellular response to oxidative stress GO:0034599 TAS
    negative regulation of hydrogen peroxide-induced cell death GO:1903206 IGI
    regulation of embryonic development GO:0045995 IEA
    cellular response to fluid shear stress GO:0071498 IDA
    positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress GO:0036091 IEA
    positive regulation of transcription from RNA polymerase II promoter in response to stress GO:0036003 IMP
Subcellular Localization
    chromatin GO:0000785 IEA
    nucleoplasm GO:0005654 IDA
    plasma membrane GO:0005886 IDA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IDA
    cytoplasm GO:0005737 IDA
    protein-DNA complex GO:0032993 ISS
    centrosome GO:0005813 IDA
 Experiment description of studies that identified NFE2L2 in exosomes
1
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NFE2L2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PMF1  
Two-hybrid Homo sapiens
2 PPARG 5468
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
3 NFE2  
Affinity Capture-MS Homo sapiens
4 ATF4  
Affinity Capture-MS Homo sapiens
5 MAFK 7975
Invivo Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
6 EIF2AK3  
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
7 JUND 3727
Invivo Homo sapiens
8 KEAP1 9817
Reconstituted Complex Homo sapiens
9 MAFF  
Reconstituted Complex Homo sapiens
10 Keap1  
Reconstituted Complex Mus musculus
Two-hybrid Mus musculus
Phenotypic Suppression Mus musculus
11 CASP1  
Invivo Homo sapiens
Two-hybrid Homo sapiens
12 CREBBP  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 MAFG  
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
14 CASP3 836
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which NFE2L2 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here