Gene description for RAP1GAP
Gene name RAP1 GTPase activating protein
Gene symbol RAP1GAP
Other names/aliases RAP1GA1
RAP1GAP1
RAP1GAPII
RAPGAP
Species Homo sapiens
 Database cross references - RAP1GAP
ExoCarta ExoCarta_5909
Vesiclepedia VP_5909
Entrez Gene 5909
HGNC 9858
MIM 600278
UniProt P47736  
 RAP1GAP identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for RAP1GAP
Molecular Function
    GTPase activity GO:0003924 NAS
    GTPase activator activity GO:0005096 EXP
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IDA
    protein binding GO:0005515 IPI
    small GTPase binding GO:0031267 IPI
    protein homodimerization activity GO:0042803 IPI
Biological Process
    adaptive immune response GO:0002250 TAS
    signal transduction GO:0007165 NAS
    regulation of GTPase activity GO:0043087 IDA
    positive regulation of GTPase activity GO:0043547 IDA
    negative regulation of neuron differentiation GO:0045665 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 IEA
    negative regulation of microvillus assembly GO:1903697 IMP
    negative regulation of thyroid gland epithelial cell proliferation GO:1904442 IEA
    cellular response to glial cell derived neurotrophic factor GO:1990792 IEA
Subcellular Localization
    Golgi membrane GO:0000139 IEA
    cytoplasm GO:0005737 IBA
    early endosome GO:0005769 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 IBA
    membrane GO:0016020 IDA
    axon GO:0030424 IEA
    dendrite GO:0030425 IEA
    neuronal cell body GO:0043025 IEA
 Experiment description of studies that identified RAP1GAP in exosomes
1
Experiment ID 236
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for RAP1GAP
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GNAZ 2781
Reconstituted Complex Homo sapiens
2 PFN2 5217
Two-hybrid Homo sapiens
3 BTRC 8945
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 FBXW11  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
5 RAP1GAP2 23108
Affinity Capture-MS Homo sapiens
6 FBXO7 25793
Affinity Capture-MS Homo sapiens
7 TP53 7157
Affinity Capture-MS Homo sapiens
8 XPO1 7514
Affinity Capture-MS Homo sapiens
9 ATE1 11101
Affinity Capture-MS Homo sapiens
10 CSNK1G2 1455
Two-hybrid Homo sapiens
11 IFI30  
Affinity Capture-MS Homo sapiens
12 DYNC1I1  
Two-hybrid Homo sapiens
13 UBE3A 7337
Affinity Capture-Western Homo sapiens
14 ZNF330  
Affinity Capture-MS Homo sapiens
15 SKAP2 8935
Affinity Capture-MS Homo sapiens
16 CLTCL1 8218
Affinity Capture-MS Homo sapiens
17 APEX1 328
Affinity Capture-RNA Homo sapiens
18 MAP1LC3B 81631
Two-hybrid Homo sapiens
19 VPS26A 9559
Affinity Capture-MS Homo sapiens
20 MAPK6  
Two-hybrid Homo sapiens
21 CNR1  
Affinity Capture-Western Homo sapiens
22 MEX3B 84206
Two-hybrid Homo sapiens
23 EZH2  
Affinity Capture-MS Homo sapiens
24 MLLT4 4301
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 RPA3 6119
Proximity Label-MS Homo sapiens
26 RAP1A 5906
Biochemical Activity Homo sapiens
27 PLK1 5347
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
28 BRAF  
Reconstituted Complex Homo sapiens
29 GNAI3 2773
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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