Gene description for RAPGEF1
Gene name Rap guanine nucleotide exchange factor (GEF) 1
Gene symbol RAPGEF1
Other names/aliases C3G
GRF2
Species Homo sapiens
 Database cross references - RAPGEF1
ExoCarta ExoCarta_2889
Entrez Gene 2889
HGNC 4568
MIM 600303
UniProt Q13905  
 RAPGEF1 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
Prostate cancer cells 25844599    
 Gene ontology annotations for RAPGEF1
Molecular Function
    Rap guanyl-nucleotide exchange factor activity GO:0017034 IMP
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
Biological Process
    negative regulation of protein kinase B signaling GO:0051898 IEA
    regulation of cell junction assembly GO:1901888 IMP
    single organismal cell-cell adhesion GO:0016337 IEA
    transmembrane receptor protein tyrosine kinase signaling pathway GO:0007169 TAS
    establishment of endothelial barrier GO:0061028 IMP
    platelet-derived growth factor receptor signaling pathway GO:0048008 IEA
    activation of MAPKK activity GO:0000186 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    nerve growth factor signaling pathway GO:0038180 IDA
    blood vessel development GO:0001568 IEA
    negative regulation of neural precursor cell proliferation GO:2000178 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IEA
    positive regulation of GTPase activity GO:0043547 IMP
    negative regulation of Ras protein signal transduction GO:0046580 IEA
    signal transduction GO:0007165 NAS
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    cellular response to nerve growth factor stimulus GO:1990090 IDA
    Rap protein signal transduction GO:0032486 IMP
    cellular response to cAMP GO:0071320 IDA
    positive regulation of neuron projection development GO:0010976 IMP
Subcellular Localization
    cytosol GO:0005829 TAS
    endosome GO:0005768 TAS
    early endosome GO:0005769 IDA
 Experiment description of studies that identified RAPGEF1 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
2
Experiment ID 274
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|RAB5A
EV Cytosolic markers
CD9|CD82|CD63|CD81
EV Membrane markers
AIF
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for RAPGEF1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NEDD9 4739
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
2 BCAR1 9564
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 CRKL 1399
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 GRB2 2885
Affinity Capture-Western Homo sapiens
5 SHC1 6464
Invivo Homo sapiens
6 HCK 3055
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 CRK 1398
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which RAPGEF1 is involved
PathwayEvidenceSource
Downstream signal transduction TAS Reactome
Frs2-mediated activation TAS Reactome
Regulation of signaling by CBL TAS Reactome





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