Experiment description of studies that identified PSMB9 in exosomes |
1 |
Experiment ID |
79 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD81|MHCII
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20458337
|
Organism |
Homo sapiens |
Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 |
Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
Journal name |
ICB
|
Publication year |
2010 |
Sample |
B cells |
Sample name |
RN (HLA-DR15) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [FT-ICR] Western blotting |
|
|
2 |
Experiment ID |
80 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD81|MHCII
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20458337
|
Organism |
Homo sapiens |
Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 |
Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
Journal name |
ICB
|
Publication year |
2010 |
Sample |
B cells |
Sample name |
RN (HLA-DR15) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [FT-ICR] Western blotting |
|
|
3 |
Experiment ID |
81 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD81|MHCII
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20458337
|
Organism |
Homo sapiens |
Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 |
Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
Journal name |
ICB
|
Publication year |
2010 |
Sample |
B cells |
Sample name |
RN (HLA-DR15) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [FT-ICR] Western blotting |
|
|
4 |
Experiment ID |
126 |
ISEV standards |
✘
|
EV Biophysical techniques |
✔
GAPDH
|
EV Cytosolic markers |
✘
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
PubMed ID |
Unpublished / Not applicable
|
Organism |
Homo sapiens |
Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
Authors |
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim |
Journal name |
Mesenchymal Stem Cell Therapy
|
Publication year |
2011 |
Sample |
Mesenchymal stem cells |
Sample name |
huES9.E1 |
Isolation/purification methods |
HPLC |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Western blotting Antobody array Mass spectrometry |
|
|
5 |
Experiment ID |
140 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
VCaP - Rep 2 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
6 |
Experiment ID |
141 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
HSP70|HSP90|RAB5
|
EV Cytosolic markers |
✔
LAMP2|CD9
|
EV Membrane markers |
✔
HSP90B1
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
|
PubMed ID |
22723089
|
Organism |
Homo sapiens |
Experiment description |
Prostate cancer cell derived exosomes |
Authors |
Hosseini-Beheshti E, Guns ES. |
Journal name |
MCP
|
Publication year |
2012 |
Sample |
Prostate cancer cells |
Sample name |
VCaP - Rep 3 |
Isolation/purification methods |
Sucrose density gradient |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [QTOF] |
|
|
7 |
Experiment ID |
217 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
CD81|CD9|CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23844026
|
Organism |
Homo sapiens |
Experiment description |
Characterization of human thymic exosomes. |
Authors |
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. |
Journal name |
PLoS One
|
Publication year |
2013 |
Sample |
Thymus |
Sample name |
Normal-Thymus |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|