Gene description for RAD23A
Gene name RAD23 homolog A (S. cerevisiae)
Gene symbol RAD23A
Other names/aliases HHR23A
HR23A
Species Homo sapiens
 Database cross references - RAD23A
ExoCarta ExoCarta_5886
Vesiclepedia VP_5886
Entrez Gene 5886
HGNC 9812
MIM 600061
UniProt P54725  
 RAD23A identified in sEVs derived from the following tissue/cell type
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
 Gene ontology annotations for RAD23A
Molecular Function
    damaged DNA binding GO:0003684 IEA
    single-stranded DNA binding GO:0003697 TAS
    protein binding GO:0005515 IPI
    kinase binding GO:0019900 IPI
    polyubiquitin modification-dependent protein binding GO:0031593 IBA
    polyubiquitin modification-dependent protein binding GO:0031593 IDA
    ubiquitin binding GO:0043130 IBA
    proteasome binding GO:0070628 IBA
    ubiquitin-specific protease binding GO:1990381 IPI
Biological Process
    nucleotide-excision repair GO:0006289 IDA
    protein destabilization GO:0031648 IMP
    regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032434 IDA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IMP
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    positive regulation of viral genome replication GO:0045070 IMP
    positive regulation of cell cycle GO:0045787 IMP
Subcellular Localization
    proteasome complex GO:0000502 IEA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IBA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    protein-containing complex GO:0032991 IMP
    intracellular membrane-bounded organelle GO:0043231 IDA
 Experiment description of studies that identified RAD23A in sEVs
1
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for RAD23A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA6 5687
Affinity Capture-MS Homo sapiens
2 TNFAIP3 7128
Two-hybrid Homo sapiens
3 XPC  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
4 CDC34 997
Co-fractionation Homo sapiens
5 ISG15 9636
Affinity Capture-MS Homo sapiens
6 PSMD6 9861
Affinity Capture-MS Homo sapiens
7 OGFOD1  
Co-fractionation Homo sapiens
8 USP13  
Affinity Capture-MS Homo sapiens
9 PSMA4 5685
Affinity Capture-MS Homo sapiens
10 MPG 4350
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
11 PSMD11 5717
Affinity Capture-MS Homo sapiens
12 YWHAE 7531
Co-fractionation Homo sapiens
13 USP5 8078
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
14 SH3RF1  
Two-hybrid Homo sapiens
15 UBA1 7317
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
16 EPS15 2060
Affinity Capture-MS Homo sapiens
17 UBE3A 7337
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
18 LSM4 25804
Co-fractionation Homo sapiens
19 UBC 7316
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
20 HSPB1 3315
Two-hybrid Homo sapiens
21 BASP1 10409
Affinity Capture-MS Homo sapiens
22 TRIM55  
Two-hybrid Homo sapiens
23 LOXL4 84171
Two-hybrid Homo sapiens
24 SRSF2 6427
Co-fractionation Homo sapiens
25 TRIM54  
Two-hybrid Homo sapiens
26 NPM2  
Affinity Capture-MS Homo sapiens
27 PARK2  
Biochemical Activity Homo sapiens
28 CORO1B 57175
Co-fractionation Homo sapiens
29 WWP2 11060
Biochemical Activity Homo sapiens
30 IARS2 55699
Co-fractionation Homo sapiens
31 MORC3 23515
Affinity Capture-MS Homo sapiens
32 TRIM50  
Two-hybrid Homo sapiens
33 ERC1 23085
Affinity Capture-MS Homo sapiens
34 HERC4 26091
Co-fractionation Homo sapiens
35 PSMB6 5694
Affinity Capture-MS Homo sapiens
36 IFIT3 3437
Two-hybrid Homo sapiens
37 EWSR1 2130
Two-hybrid Homo sapiens
38 PSMC2 5701
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 UBQLN1 29979
Co-fractionation Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
40 SPANXN3  
Affinity Capture-MS Homo sapiens
41 FAF1 11124
Affinity Capture-MS Homo sapiens
42 UCHL5 51377
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 NGLY1 55768
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 PSMD5 5711
Affinity Capture-MS Homo sapiens
45 SZT2 23334
Affinity Capture-MS Homo sapiens
46 UBQLN4 56893
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
47 UBAC1 10422
Affinity Capture-MS Homo sapiens
48 PSMB3 5691
Affinity Capture-MS Homo sapiens
49 RAD23A 5886
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
50 UIMC1  
Affinity Capture-MS Homo sapiens
51 HSPA8 3312
Affinity Capture-MS Homo sapiens
52 TRIM8  
Two-hybrid Homo sapiens
53 RABGEF1 27342
Two-hybrid Homo sapiens
54 UBR4 23352
Affinity Capture-MS Homo sapiens
55 CAMK2A 815
Affinity Capture-Western Homo sapiens
56 RAD23B 5887
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
57 RNF41  
Two-hybrid Homo sapiens
58 TACC3  
Co-fractionation Homo sapiens
59 UBE4B 10277
Reconstituted Complex Homo sapiens
60 PSMB4 5692
Affinity Capture-MS Homo sapiens
61 ANKRD40 91369
Two-hybrid Homo sapiens
62 DAZAP2  
Two-hybrid Homo sapiens
63 RNF32  
Two-hybrid Homo sapiens
64 USP14 9097
Affinity Capture-MS Homo sapiens
65 PSMD4 5710
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
66 IFIT5 24138
Two-hybrid Homo sapiens
67 PHAX  
Two-hybrid Homo sapiens
68 CLNS1A 1207
Co-fractionation Homo sapiens
69 A2M 2
Two-hybrid Homo sapiens
70 PSMC1 5700
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
71 PSMD14 10213
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 RNF115  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
73 DIABLO 56616
Co-fractionation Homo sapiens
74 BRCA2 675
Affinity Capture-MS Homo sapiens
75 GCC2 9648
Affinity Capture-MS Homo sapiens
76 MDM2  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
77 GTF2H1 2965
Reconstituted Complex Homo sapiens
78 PSMD2 5708
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
79 VCP 7415
Affinity Capture-Western Homo sapiens
80 OGT 8473
Reconstituted Complex Homo sapiens
81 REL 5966
Two-hybrid Homo sapiens
82 CDKN3 1033
Affinity Capture-MS Homo sapiens
83 CHEK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
84 HUWE1 10075
Affinity Capture-MS Homo sapiens
85 FH 2271
Co-fractionation Homo sapiens
86 ACBD5 91452
Proximity Label-MS Homo sapiens
87 VAV2 7410
Two-hybrid Homo sapiens
88 CEP76  
Affinity Capture-MS Homo sapiens
89 CLDN19  
Affinity Capture-MS Homo sapiens
90 ARL4C 10123
Affinity Capture-MS Homo sapiens
91 ALDH2 217
Co-fractionation Homo sapiens
92 PSMD12 5718
Affinity Capture-MS Homo sapiens
93 ZBTB44  
Two-hybrid Homo sapiens
94 ECH1 1891
Co-fractionation Homo sapiens
95 UBE2N 7334
Affinity Capture-MS Homo sapiens
96 PSMA3 5684
Affinity Capture-MS Homo sapiens
97 CCNA2 890
Reconstituted Complex Homo sapiens
98 PSEN1 5663
Two-hybrid Homo sapiens
99 WARS 7453
Co-fractionation Homo sapiens
100 OTUD1 220213
Affinity Capture-MS Homo sapiens
101 COL20A1  
Affinity Capture-MS Homo sapiens
102 TBCE 6905
Co-fractionation Homo sapiens
103 UBQLN2 29978
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
104 EEF2 1938
Affinity Capture-MS Homo sapiens
105 TRAF6 7189
Two-hybrid Homo sapiens
106 PSMA2 5683
Affinity Capture-MS Homo sapiens
107 SH3RF2  
Affinity Capture-MS Homo sapiens
108 DLG4  
Affinity Capture-Western Homo sapiens
109 PSMD13 5719
Affinity Capture-MS Homo sapiens
110 PI4K2A 55361
Two-hybrid Homo sapiens
111 SORD 6652
Co-fractionation Homo sapiens
112 TRAF3 7187
Two-hybrid Homo sapiens
113 UBE2R2 54926
Co-fractionation Homo sapiens
114 EEF1A1 1915
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
115 HEXA 3073
Co-fractionation Homo sapiens
116 MAP3K1 4214
Biochemical Activity Homo sapiens
117 NT5C2 22978
Co-fractionation Homo sapiens
118 CEP250 11190
Affinity Capture-MS Homo sapiens
119 PTTG1  
Affinity Capture-Western Homo sapiens
120 LACRT 90070
Affinity Capture-MS Homo sapiens
121 MYO6 4646
Co-fractionation Homo sapiens
122 PCDH10 57575
Affinity Capture-Western Homo sapiens
123 CNTROB  
Two-hybrid Homo sapiens
124 DNAJC9 23234
Affinity Capture-MS Homo sapiens
125 UBA6 55236
Co-fractionation Homo sapiens
126 TMOD3 29766
Co-fractionation Homo sapiens
127 TRAF5  
Two-hybrid Homo sapiens
128 TXNL1 9352
Affinity Capture-MS Homo sapiens
129 PSMC5 5705
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
130 UBL7  
Two-hybrid Homo sapiens
131 PSMB5 5693
Affinity Capture-MS Homo sapiens
132 FAM188A 80013
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
133 TRNT1 51095
Co-fractionation Homo sapiens
134 TRIM39  
Two-hybrid Homo sapiens
135 CETN2 1069
Affinity Capture-MS Homo sapiens
136 GNPDA1 10007
Co-fractionation Homo sapiens
137 PSMD7 5713
Affinity Capture-MS Homo sapiens
138 PSMB1 5689
Affinity Capture-MS Homo sapiens
139 UBXN1 51035
Two-hybrid Homo sapiens
140 XRCC5 7520
Affinity Capture-MS Homo sapiens
141 DCTPP1 79077
Co-fractionation Homo sapiens
142 PHGDH 26227
Co-fractionation Homo sapiens
143 TNNT1  
Affinity Capture-MS Homo sapiens
144 PSMC3 5702
Affinity Capture-MS Homo sapiens
145 GTF3C4 9329
Co-fractionation Homo sapiens
146 PSMB2 5690
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
147 CDC25A  
Affinity Capture-MS Homo sapiens
148 TRIP6 7205
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
149 RPS27A 6233
Two-hybrid Homo sapiens
150 MKRN1 23608
Two-hybrid Homo sapiens
151 TRAF2 7186
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
152 PARP1 142
Affinity Capture-MS Homo sapiens
153 USP25  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
154 TP53 7157
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
155 PYGL 5836
Co-fractionation Homo sapiens
156 PCMT1 5110
Co-fractionation Homo sapiens
157 ZBTB8A  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
158 VIM 7431
Affinity Capture-Western Homo sapiens
159 PSMB7 5695
Co-fractionation Homo sapiens
160 ATXN7  
Two-hybrid Homo sapiens
161 MEN1 4221
Affinity Capture-MS Homo sapiens
162 CDC37 11140
Two-hybrid Homo sapiens
163 UBA52 7311
Two-hybrid Homo sapiens
164 ZBTB26  
Two-hybrid Homo sapiens
165 SMURF1 57154
Biochemical Activity Homo sapiens
166 SQSTM1 8878
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
167 PSMD8 5714
Affinity Capture-MS Homo sapiens
168 SHFM1 7979
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
169 FKBP9 11328
Co-fractionation Homo sapiens
170 CREBBP  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
171 FILIP1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
172 DDX58 23586
Affinity Capture-RNA Homo sapiens
173 PDE6D 5147
Two-hybrid Homo sapiens
174 PSMA1 5682
Affinity Capture-MS Homo sapiens
175 PCNA 5111
Co-fractionation Homo sapiens
176 NUTM2F  
Two-hybrid Homo sapiens
177 CEBPA  
Protein-peptide Homo sapiens
178 PSMA5 5686
Affinity Capture-MS Homo sapiens
179 KRTAP12-2  
Two-hybrid Homo sapiens
180 PSMA7 5688
Affinity Capture-MS Homo sapiens
181 DBI 1622
Two-hybrid Homo sapiens
182 PRDX1 5052
Co-fractionation Homo sapiens
183 SHARPIN  
Affinity Capture-MS Homo sapiens
184 MEA1  
Affinity Capture-MS Homo sapiens
185 ADRM1 11047
Affinity Capture-MS Homo sapiens
186 SUMO3 6612
Co-fractionation Homo sapiens
187 PSMD1 5707
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
188 ATXN3 4287
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
189 TEX33  
Affinity Capture-MS Homo sapiens
190 PSMC4 5704
Affinity Capture-MS Homo sapiens
191 MAST1  
Two-hybrid Homo sapiens
192 ALDH1B1 219
Co-fractionation Homo sapiens
193 NEDD8 4738
Protein-peptide Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
194 ECSIT 51295
Two-hybrid Homo sapiens
195 PSMD3 5709
Affinity Capture-MS Homo sapiens
196 PSME4 23198
Affinity Capture-MS Homo sapiens
197 ARL4D  
Affinity Capture-MS Homo sapiens
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