Gene description for TRIM5
Gene name tripartite motif containing 5
Gene symbol TRIM5
Other names/aliases RNF88
TRIM5alpha
Species Homo sapiens
 Database cross references - TRIM5
ExoCarta ExoCarta_85363
Vesiclepedia VP_85363
Entrez Gene 85363
HGNC 16276
MIM 608487
UniProt Q9C035  
 TRIM5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for TRIM5
Molecular Function
    transcription coactivator activity GO:0003713 IDA
    ubiquitin-protein transferase activity GO:0004842 IDA
    protein binding GO:0005515 IPI
    zinc ion binding GO:0008270 IEA
    protein kinase binding GO:0019901 IBA
    protein kinase binding GO:0019901 IPI
    protein-macromolecule adaptor activity GO:0030674 IPI
    pattern recognition receptor activity GO:0038187 IDA
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IBA
    protein homodimerization activity GO:0042803 IPI
    ubiquitin protein ligase activity GO:0061630 IBA
Biological Process
    activation of innate immune response GO:0002218 IDA
    autophagy GO:0006914 IDA
    regulation of gene expression GO:0010468 IBA
    regulation of lipopolysaccharide-mediated signaling pathway GO:0031664 IMP
    regulation of protein localization GO:0032880 IBA
    regulation of protein localization GO:0032880 IMP
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IBA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IDA
    positive regulation of canonical NF-kappaB signal transduction GO:0043123 IMP
    positive regulation of MAPK cascade GO:0043410 IMP
    suppression of viral release by host GO:0044790 IDA
    innate immune response GO:0045087 IBA
    innate immune response GO:0045087 IDA
    positive regulation of DNA-templated transcription GO:0045893 IEA
    regulation of viral entry into host cell GO:0046596 IBA
    negative regulation of viral entry into host cell GO:0046597 IDA
    positive regulation of DNA-binding transcription factor activity GO:0051091 IMP
    positive regulation of NF-kappaB transcription factor activity GO:0051092 IMP
    defense response to virus GO:0051607 TAS
    protein K63-linked ubiquitination GO:0070534 IDA
Subcellular Localization
    P-body GO:0000932 IDA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    omegasome GO:1990462 IDA
 Experiment description of studies that identified TRIM5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TRIM5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MPP7 143098
Two-hybrid Homo sapiens
2 UBE2H 7328
Two-hybrid Homo sapiens
3 TRIM5 85363
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
4 ZWINT  
Affinity Capture-MS Homo sapiens
5 USP2  
Two-hybrid Homo sapiens
6 UBE2N 7334
Two-hybrid Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
7 PHB2 11331
Affinity Capture-Western Homo sapiens
8 DCXR 51181
Affinity Capture-MS Homo sapiens
9 LGALS8 3964
Reconstituted Complex Homo sapiens
10 LRPPRC 10128
Affinity Capture-Western Homo sapiens
11 CANX 821
Co-localization Homo sapiens
12 YES1 7525
Two-hybrid Homo sapiens
13 KIF14 9928
Affinity Capture-MS Homo sapiens
14 TNFAIP3 7128
Two-hybrid Homo sapiens
15 TAB2  
Affinity Capture-Western Homo sapiens
16 XPO1 7514
Affinity Capture-MS Homo sapiens
17 USP15 9958
Two-hybrid Homo sapiens
18 UBE2V1 7335
Two-hybrid Homo sapiens
19 USP5 8078
Two-hybrid Homo sapiens
20 ATG13 9776
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 EHHADH 1962
Two-hybrid Homo sapiens
22 LGALS3 3958
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 MAP3K11 4296
Affinity Capture-Western Homo sapiens
24 IKBKG 8517
Affinity Capture-Western Homo sapiens
25 TSGA10IP  
Two-hybrid Homo sapiens
26 CUL3 8452
Affinity Capture-MS Homo sapiens
27 KIF20A 10112
Affinity Capture-MS Homo sapiens
28 SQSTM1 8878
Affinity Capture-Western Homo sapiens
29 MKRN3  
Two-hybrid Homo sapiens
30 UBC 7316
Biochemical Activity Homo sapiens
31 USP21  
Two-hybrid Homo sapiens
32 MAP3K1 4214
Affinity Capture-Western Homo sapiens
33 TFPT  
Affinity Capture-MS Homo sapiens
34 TRIM6  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
35 TAB3 257397
Affinity Capture-Western Homo sapiens
36 CREB1  
Affinity Capture-MS Homo sapiens
37 Ppp2ca 19052
Affinity Capture-MS Mus musculus
38 PSMD7 5713
Affinity Capture-Western Homo sapiens
39 FAM9C  
Affinity Capture-MS Homo sapiens
40 RB1CC1 9821
Affinity Capture-Western Homo sapiens
41 PRKCG 5582
Reconstituted Complex Homo sapiens
42 CUEDC1 404093
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 UBB 7314
Two-hybrid Homo sapiens
44 UBE2D2 7322
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
45 OTUD6A  
Two-hybrid Homo sapiens
46 TRIM32 22954
Two-hybrid Homo sapiens
47 UBE2D4  
Two-hybrid Homo sapiens
48 MNAT1  
Two-hybrid Homo sapiens
49 UBASH3A 53347
Two-hybrid Homo sapiens
50 WDYHV1  
Two-hybrid Homo sapiens
51 MAP3K7  
Affinity Capture-Western Homo sapiens
52 TRIM21 6737
Reconstituted Complex Homo sapiens
53 MTURN  
Two-hybrid Homo sapiens
54 UBE2K 3093
Two-hybrid Homo sapiens
55 YARS 8565
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 TRIM35 23087
Reconstituted Complex Homo sapiens
57 USP39 10713
Two-hybrid Homo sapiens
58 TRIM34  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
59 PCBD1 5092
Two-hybrid Homo sapiens
60 PSMC5 5705
Affinity Capture-Western Homo sapiens
61 ARRDC3 57561
Affinity Capture-MS Homo sapiens
62 PIAS1 8554
Biochemical Activity Homo sapiens
63 SGTA 6449
Two-hybrid Homo sapiens
64 UBE2D3 7323
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
65 OTUB2  
Two-hybrid Homo sapiens
66 LZTR1 8216
Two-hybrid Homo sapiens
67 NFYA 4800
Co-fractionation Homo sapiens
68 EWSR1 2130
Two-hybrid Homo sapiens
69 PSMC4 5704
Affinity Capture-Western Homo sapiens
70 PSMC2 5701
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
71 TRIM6-TRIM34  
Affinity Capture-MS Homo sapiens
72 NCOA4 8031
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
73 UBE2V2 7336
Two-hybrid Homo sapiens
74 UBE2U  
Two-hybrid Homo sapiens
75 N4BP1  
Affinity Capture-Western Homo sapiens
76 SUMO1 7341
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
77 UBE2D1 7321
Two-hybrid Homo sapiens
78 GBAS 2631
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which TRIM5 is involved
PathwayEvidenceSource
Cytokine Signaling in Immune system TAS Reactome
Immune System TAS Reactome
Interferon gamma signaling TAS Reactome
Interferon Signaling TAS Reactome





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