Gene description for MBD2
Gene name methyl-CpG binding domain protein 2
Gene symbol MBD2
Other names/aliases DMTase
NY-CO-41
Species Homo sapiens
 Database cross references - MBD2
ExoCarta ExoCarta_8932
Vesiclepedia VP_8932
Entrez Gene 8932
HGNC 6917
MIM 603547
UniProt Q9UBB5  
 MBD2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MBD2
Molecular Function
    satellite DNA binding GO:0003696 TAS
    mRNA binding GO:0003729 IEA
    protein binding GO:0005515 IPI
    methyl-CpG binding GO:0008327 IBA
    methyl-CpG binding GO:0008327 IDA
    methyl-CpG binding GO:0008327 NAS
    protein domain specific binding GO:0019904 IPI
    nucleosomal DNA binding GO:0031492 HDA
    siRNA binding GO:0035197 IEA
    identical protein binding GO:0042802 IEA
    molecular adaptor activity GO:0060090 EXP
    C2H2 zinc finger domain binding GO:0070742 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IBA
    negative regulation of transcription by RNA polymerase II GO:0000122 IGI
    ventricular cardiac muscle tissue development GO:0003229 IEA
    chromatin remodeling GO:0006338 HDA
    chromatin remodeling GO:0006338 IDA
    DNA methylation-dependent constitutive heterochromatin formation GO:0006346 IBA
    DNA methylation-dependent constitutive heterochromatin formation GO:0006346 IGI
    response to mechanical stimulus GO:0009612 IEA
    Wnt signaling pathway GO:0016055 IEA
    positive regulation of Wnt signaling pathway GO:0030177 IEA
    response to nutrient levels GO:0031667 IEA
    response to estradiol GO:0032355 IEA
    regulation of cell population proliferation GO:0042127 IEA
    maternal behavior GO:0042711 IEA
    negative regulation of DNA-templated transcription GO:0045892 NAS
    positive regulation of DNA-templated transcription GO:0045893 NAS
    embryonic organ development GO:0048568 IEA
    protein-containing complex assembly GO:0065003 IEA
    cellular response to organic cyclic compound GO:0071407 IEA
Subcellular Localization
    chromatin GO:0000785 HDA
    chromatin GO:0000785 IDA
    heterochromatin GO:0000792 IEA
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 IDA
    NuRD complex GO:0016581 IDA
    NuRD complex GO:0016581 NAS
    protein-containing complex GO:0032991 HDA
 Experiment description of studies that identified MBD2 in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MBD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CDCA5  
Affinity Capture-MS Homo sapiens
2 CHAF1A  
Affinity Capture-MS Homo sapiens
3 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
4 HNRNPC 3183
Co-fractionation Homo sapiens
5 GATAD2B 57459
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 HDAC2 3066
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
7 KPNA1 3836
Affinity Capture-MS Homo sapiens
8 ZNF296  
Affinity Capture-MS Homo sapiens
9 C9orf78 51759
Affinity Capture-MS Homo sapiens
10 SAP30 8819
Affinity Capture-MS Homo sapiens
11 Cdk2ap1  
Affinity Capture-MS Mus musculus
12 INSL3  
Affinity Capture-MS Homo sapiens
13 csul  
Biochemical Activity Drosophila melanogaster
14 BDNF 627
Co-localization Homo sapiens
15 CDK2AP1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 RELN 5649
Co-localization Homo sapiens
17 ARID4B  
Affinity Capture-MS Homo sapiens
18 ZNF512B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 SIN3B  
Affinity Capture-MS Homo sapiens
20 MBD3L2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
21 LAMTOR3 8649
Two-hybrid Homo sapiens
22 HINFP  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 EED  
Affinity Capture-MS Homo sapiens
24 ZNF219  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 PHF1  
Affinity Capture-MS Homo sapiens
26 SMARCA1 6594
Affinity Capture-MS Homo sapiens
27 MLXIPL  
Affinity Capture-MS Homo sapiens
28 SALL4  
Affinity Capture-MS Homo sapiens
29 SOX5 6660
Proximity Label-MS Homo sapiens
30 MTA2 9219
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
31 BCL11B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 RBPJ 3516
Affinity Capture-MS Homo sapiens
33 PSMC2 5701
Co-fractionation Homo sapiens
34 PHF20L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
35 RBBP7 5931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
36 SHPRH  
Affinity Capture-MS Homo sapiens
37 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
38 C17orf49 124944
Affinity Capture-MS Homo sapiens
39 LIN52  
Affinity Capture-MS Homo sapiens
40 DPY30 84661
Affinity Capture-MS Homo sapiens
41 RFX3 5991
Affinity Capture-MS Homo sapiens
42 MYH9 4627
Co-fractionation Homo sapiens
43 KDM5B  
Affinity Capture-Western Homo sapiens
44 PTK2B 2185
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
45 BPTF 2186
Affinity Capture-MS Homo sapiens
46 BRMS1L  
Affinity Capture-MS Homo sapiens
47 MYBL2 4605
Affinity Capture-MS Homo sapiens
48 MBD2 8932
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
49 CHD5 26038
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 DHX9 1660
Two-hybrid Homo sapiens
51 SUDS3  
Affinity Capture-MS Homo sapiens
52 MRPL22  
Affinity Capture-MS Homo sapiens
53 PHF12  
Affinity Capture-MS Homo sapiens
54 CEBPA  
Proximity Label-MS Homo sapiens
55 RAD21 5885
Co-fractionation Homo sapiens
56 PURB 5814
Two-hybrid Homo sapiens
57 BCL11A 53335
Affinity Capture-MS Homo sapiens
58 ZBTB2 57621
Affinity Capture-MS Homo sapiens
59 HIST1H3A 8350
Proximity Label-MS Homo sapiens
60 ACTL6A 86
Co-fractionation Homo sapiens
61 STMN2  
Affinity Capture-MS Homo sapiens
62 SOX6  
Proximity Label-MS Homo sapiens
63 SMARCA5 8467
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
64 IKZF3  
Affinity Capture-MS Homo sapiens
65 TFAP4 7023
Affinity Capture-MS Homo sapiens
66 GPN1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
67 IKZF1  
Affinity Capture-MS Homo sapiens
68 SUZ12  
Affinity Capture-MS Homo sapiens
69 Max  
Affinity Capture-MS Mus musculus
70 TBP  
Reconstituted Complex Homo sapiens
71 PRMT5 10419
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
72 LIN9  
Affinity Capture-MS Homo sapiens
73 Mta2 23942
Affinity Capture-MS Mus musculus
74 ZMYND8 23613
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
75 CHD3 1107
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
76 TRIM27  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
77 HJURP  
Affinity Capture-MS Homo sapiens
78 ARID1A 8289
Co-fractionation Homo sapiens
79 HAT1 8520
Affinity Capture-MS Homo sapiens
80 MBD3L1  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
81 HMG20A  
Affinity Capture-MS Homo sapiens
82 RBBP4 5928
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
83 AR 367
Proximity Label-MS Homo sapiens
84 TACC3  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
85 PSMC3 5702
Co-fractionation Homo sapiens
86 GATAD2A 54815
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
87 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
88 TRPS1  
Affinity Capture-MS Homo sapiens
89 WDR77 79084
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
90 SAP130  
Affinity Capture-MS Homo sapiens
91 SIN3A  
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
92 MBD3 53615
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
93 MTA1 9112
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
94 GAD1  
Co-localization Homo sapiens
95 HDAC1 3065
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
96 CHD4 1108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 SALL1 6299
Affinity Capture-MS Homo sapiens
98 ESR1  
Co-localization Homo sapiens
99 Mta1  
Affinity Capture-MS Mus musculus
100 HMGXB4 10042
Affinity Capture-MS Homo sapiens
101 KDM1A 23028
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
102 EZH2  
Affinity Capture-MS Homo sapiens
103 CAD 790
Co-fractionation Homo sapiens
104 MTA3 57504
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
105 GATAD1  
Affinity Capture-MS Homo sapiens
106 MTF2  
Affinity Capture-MS Homo sapiens
107 CHAF1B  
Affinity Capture-MS Homo sapiens
108 PWWP2A  
Affinity Capture-MS Homo sapiens
109 DNMT1 1786
Affinity Capture-Western Homo sapiens
110 PRMT1 3276
Affinity Capture-Western Homo sapiens
111 LIN37  
Affinity Capture-MS Homo sapiens
112 KDM5C  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here