Gene description for PSMB4
Gene name proteasome (prosome, macropain) subunit, beta type, 4
Gene symbol PSMB4
Other names/aliases HN3
HsN3
PROS-26
PROS26
Species Homo sapiens
 Database cross references - PSMB4
ExoCarta ExoCarta_5692
Entrez Gene 5692
HGNC 9541
MIM 602177
UniProt P28070  
 PSMB4 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Bladder cancer cells 20224111    
Hepatocellular carcinoma cells 26054723    
Keratinocytes 19530224    
Keratinocytes 19530224    
Mesenchymal stem cells Unpublished / Not applicable
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Prostate cancer cells 22723089    
Prostate cancer cells 22723089    
Thymus 23844026    
 Gene ontology annotations for PSMB4
Molecular Function
    lipopolysaccharide binding GO:0001530 IEA
    protein binding GO:0005515 IPI
    threonine-type endopeptidase activity GO:0004298 IEA
    endopeptidase activity GO:0004175 IBA
Biological Process
    programmed cell death GO:0012501 TAS
    G1/S transition of mitotic cell cycle GO:0000082 TAS
    regulation of apoptotic process GO:0042981 TAS
    antigen processing and presentation of peptide antigen via MHC class I GO:0002474 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I GO:0042590 TAS
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    viral process GO:0016032 TAS
    small molecule metabolic process GO:0044281 TAS
    apoptotic process GO:0006915 TAS
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IBA
    positive regulation of canonical Wnt signaling pathway GO:0090263 TAS
    regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051439 TAS
    DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest GO:0006977 TAS
    cellular nitrogen compound metabolic process GO:0034641 TAS
    proteasomal ubiquitin-independent protein catabolic process GO:0010499 IBA
    protein polyubiquitination GO:0000209 TAS
    negative regulation of inflammatory response to antigenic stimulus GO:0002862 IEA
    anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process GO:0031145 TAS
    mitotic cell cycle GO:0000278 TAS
    positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition GO:0051437 TAS
    antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent GO:0002479 TAS
    regulation of cellular amino acid metabolic process GO:0006521 TAS
    negative regulation of canonical Wnt signaling pathway GO:0090090 TAS
    negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle GO:0051436 TAS
    gene expression GO:0010467 TAS
    innate immune response GO:0045087 TAS
    negative regulation of apoptotic process GO:0043066 TAS
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    nucleus GO:0005634 TAS
    proteasome core complex GO:0005839 ISS
    proteasome complex GO:0000502 IDA
    nucleoplasm GO:0005654 TAS
    extracellular exosome GO:0070062 IDA
 Experiment description of studies that identified PSMB4 in exosomes
1
Experiment ID 79
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 76
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|GAPDH|HSP90
EV Cytosolic markers
✔
CD81|CD9|CD63|LAMP1|MHCI
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20224111    
Organism Homo sapiens
Experiment description Proteomics analysis of bladder cancer exosomes.
Authors Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A.
Journal name MCP
Publication year 2010
Sample Bladder cancer cells
Sample name HT1376
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.10-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
FACS
5
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 189
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors Chavez-Muñoz C, Kilani RT, Ghahary A.
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Differentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 190
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSC70
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19530224    
Organism Homo sapiens
Experiment description Profile of exosomes related proteins released by differentiated and undifferentiated human keratinocytes.
Authors Chavez-Muñoz C, Kilani RT, Ghahary A.
Journal name J Cell Physiol
Publication year 2009
Sample Keratinocytes
Sample name Keratinocytes - Undifferentiated
Isolation/purification methods Differential centrifugation
Filtration
Sucrose cushion
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 126
ISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
9
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 139
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name DU145 - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
12
Experiment ID 141
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP70|HSP90|RAB5
EV Cytosolic markers
✔
LAMP2|CD9
EV Membrane markers
✔
HSP90B1
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method
Mass spectrometry [QTOF]   
PubMed ID 22723089    
Organism Homo sapiens
Experiment description Prostate cancer cell derived exosomes
Authors Hosseini-Beheshti E, Guns ES.
Journal name MCP
Publication year 2012
Sample Prostate cancer cells
Sample name VCaP - Rep 3
Isolation/purification methods Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [QTOF]
13
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PSMB4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PSMA2 5683
Invivo Homo sapiens
Invitro Homo sapiens
2 SMAD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
3 PLK1 5347
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
View the network image/svg+xml
 Pathways in which PSMB4 is involved
PathwayEvidenceSource
Activation of NF-kappaB in B cells TAS Reactome
Antigen processing: Ubiquitination & Proteasome degradation TAS Reactome
APC/C:Cdc20 mediated degradation of Securin TAS Reactome
APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 TAS Reactome
Asymmetric localization of PCP proteins TAS Reactome
AUF1 (hnRNP D0) destabilizes mRNA TAS Reactome
Autodegradation of Cdh1 by Cdh1:APC/C TAS Reactome
Autodegradation of the E3 ubiquitin ligase COP1 TAS Reactome
Cdc20:Phospho-APC/C mediated degradation of Cyclin A TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
CDK-mediated phosphorylation and removal of Cdc6 TAS Reactome
CDT1 association with the CDC6:ORC:origin complex TAS Reactome
CLEC7A (Dectin-1) signaling TAS Reactome
Cross-presentation of soluble exogenous antigens (endosomes) TAS Reactome
Dectin-1 mediated noncanonical NF-kB signaling TAS Reactome
degradation of AXIN TAS Reactome
Degradation of beta-catenin by the destruction complex TAS Reactome
degradation of DVL TAS Reactome
Degradation of GLI1 by the proteasome TAS Reactome
Degradation of GLI2 by the proteasome TAS Reactome
ER-Phagosome pathway TAS Reactome
GLI3 is processed to GLI3R by the proteasome TAS Reactome
Hedgehog 'on' state TAS Reactome
Hedgehog ligand biogenesis TAS Reactome
Hh mutants that don't undergo autocatalytic processing are degraded by ERAD TAS Reactome
Orc1 removal from chromatin TAS Reactome
Orc1 removal from chromatin TAS Reactome
Regulation of activated PAK-2p34 by proteasome mediated degradation TAS Reactome
Regulation of ornithine decarboxylase (ODC) TAS Reactome
SCF(Skp2)-mediated degradation of p27/p21 TAS Reactome
SCF(Skp2)-mediated degradation of p27/p21 TAS Reactome
SCF-beta-TrCP mediated degradation of Emi1 TAS Reactome
Separation of Sister Chromatids TAS Reactome
Ubiquitin Mediated Degradation of Phosphorylated Cdc25A TAS Reactome
Ubiquitin-dependent degradation of Cyclin D1 TAS Reactome
Vif-mediated degradation of APOBEC3G TAS Reactome
Vpu mediated degradation of CD4 TAS Reactome





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