Gene description for NDC80
Gene name NDC80 kinetochore complex component
Gene symbol NDC80
Other names/aliases HEC
HEC1
HsHec1
KNTC2
TID3
hsNDC80
Species Homo sapiens
 Database cross references - NDC80
ExoCarta ExoCarta_10403
Vesiclepedia VP_10403
Entrez Gene 10403
HGNC 16909
MIM 607272
UniProt O14777  
 NDC80 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
 Gene ontology annotations for NDC80
Molecular Function
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 IMP
    cyclin binding GO:0030332 IEA
    identical protein binding GO:0042802 IPI
    kinetochore adaptor activity GO:0140483 IDA
Biological Process
    mitotic sister chromatid segregation GO:0000070 TAS
    establishment of mitotic spindle orientation GO:0000132 IMP
    mitotic cell cycle GO:0000278 TAS
    mitotic spindle organization GO:0007052 IMP
    spindle assembly involved in female meiosis I GO:0007057 IEA
    chromosome segregation GO:0007059 IMP
    chromosome segregation GO:0007059 NAS
    mitotic spindle assembly checkpoint signaling GO:0007094 NAS
    G2/MI transition of meiotic cell cycle GO:0008315 IEA
    attachment of spindle microtubules to kinetochore GO:0008608 IDA
    attachment of spindle microtubules to kinetochore GO:0008608 IMP
    skeletal muscle satellite cell proliferation GO:0014841 IEA
    regulation of protein stability GO:0031647 IEA
    centrosome duplication GO:0051298 IDA
    cell division GO:0051301 IEA
    metaphase chromosome alignment GO:0051310 IDA
    metaphase chromosome alignment GO:0051310 IMP
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 IBA
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 IDA
    attachment of mitotic spindle microtubules to kinetochore GO:0051315 IMP
    kinetochore organization GO:0051383 IEA
    positive regulation of mitotic cell cycle spindle assembly checkpoint GO:0090267 IMP
Subcellular Localization
    chromosome, centromeric region GO:0000775 TAS
    kinetochore GO:0000776 IDA
    kinetochore GO:0000776 NAS
    outer kinetochore GO:0000940 IDA
    nucleus GO:0005634 TAS
    nucleoplasm GO:0005654 IDA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
    Ndc80 complex GO:0031262 IBA
    Ndc80 complex GO:0031262 IDA
    Ndc80 complex GO:0031262 IPI
 Experiment description of studies that identified NDC80 in exosomes
1
Experiment ID 226
MISEV standards
EM
Biophysical techniques
GAPDH|CD9|FLOT1
Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
 Protein-protein interactions for NDC80
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MIS12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 RBMXL1  
Affinity Capture-MS Homo sapiens
3 C19orf44  
Proximity Label-MS Homo sapiens
4 NUF2  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
Co-fractionation Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
5 TPGS1  
Proximity Label-MS Homo sapiens
6 CAMSAP1 157922
Proximity Label-MS Homo sapiens
7 CCNH  
Two-hybrid Homo sapiens
8 KRT6B 3854
Two-hybrid Homo sapiens
9 CEP89 84902
Proximity Label-MS Homo sapiens
10 CENPE 1062
Proximity Label-MS Homo sapiens
11 CCNB2  
Proximity Label-MS Homo sapiens
12 BUB1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 ALMS1  
Proximity Label-MS Homo sapiens
14 SYCE1  
Affinity Capture-MS Homo sapiens
15 MAP9 79884
Affinity Capture-MS Homo sapiens
16 UTP11L  
Affinity Capture-MS Homo sapiens
17 DYNC1I2 1781
Proximity Label-MS Homo sapiens
18 TOP3B 8940
Affinity Capture-MS Homo sapiens
19 KLC3  
Two-hybrid Homo sapiens
20 CENPC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 NSL1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
22 SFPQ 6421
Affinity Capture-MS Homo sapiens
23 RPS19 6223
Affinity Capture-MS Homo sapiens
24 Ndc80  
Affinity Capture-MS Mus musculus
25 BUB1B  
Affinity Capture-MS Homo sapiens
26 KIZ  
Proximity Label-MS Homo sapiens
27 CALCOCO1 57658
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
28 SPICE1  
Proximity Label-MS Homo sapiens
29 Soga1  
Affinity Capture-MS Mus musculus
30 SYNE2 23224
Proximity Label-MS Homo sapiens
31 MYO5C 55930
Proximity Label-MS Homo sapiens
32 CAMSAP2  
Proximity Label-MS Homo sapiens
33 KIF7 374654
Proximity Label-MS Homo sapiens
34 CEP128  
Proximity Label-MS Homo sapiens
35 NHS 4810
Proximity Label-MS Homo sapiens
36 SNX6 58533
Proximity Label-MS Homo sapiens
37 STX11 8676
Two-hybrid Homo sapiens
38 CEP97 79598
Proximity Label-MS Homo sapiens
39 PMF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 TNIP1 10318
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
41 HMMR  
Proximity Label-MS Homo sapiens
42 PIBF1  
Proximity Label-MS Homo sapiens
43 PPP1CC 5501
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 HSPA4 3308
Affinity Capture-MS Homo sapiens
45 CCNK  
Two-hybrid Homo sapiens
46 CENPU  
Affinity Capture-MS Homo sapiens
47 SCLT1 132320
Proximity Label-MS Homo sapiens
48 NDE1 54820
Two-hybrid Homo sapiens
49 HAUS6  
Proximity Label-MS Homo sapiens
50 TBC1D31  
Proximity Label-MS Homo sapiens
51 SKA1  
Two-hybrid Homo sapiens
Co-localization Homo sapiens
52 CEP95  
Proximity Label-MS Homo sapiens
53 EXOC1 55763
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 CCDC14  
Proximity Label-MS Homo sapiens
55 ATXN2L 11273
Affinity Capture-MS Homo sapiens
56 GOLGA3 2802
Proximity Label-MS Homo sapiens
57 NAV1 89796
Proximity Label-MS Homo sapiens
58 CSNK1E 1454
Proximity Label-MS Homo sapiens
59 CASC5  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 DIAPH3 81624
Proximity Label-MS Homo sapiens
61 CCDC138  
Proximity Label-MS Homo sapiens
62 INPP1 3628
Two-hybrid Homo sapiens
63 HGS 9146
Two-hybrid Homo sapiens
64 CPSF7 79869
Affinity Capture-MS Homo sapiens
65 CCDC53 51019
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
66 PLEKHG1  
Proximity Label-MS Homo sapiens
67 BLOC1S6  
Two-hybrid Homo sapiens
68 TFIP11  
Two-hybrid Homo sapiens
69 PSMC2 5701
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-fractionation Homo sapiens
70 IFT81 28981
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
71 DLG5 9231
Proximity Label-MS Homo sapiens
72 KRT78 196374
Affinity Capture-MS Homo sapiens
73 ATP5O 539
Two-hybrid Homo sapiens
74 CEP162  
Proximity Label-MS Homo sapiens
75 HERC2 8924
Proximity Label-MS Homo sapiens
76 CEP295  
Proximity Label-MS Homo sapiens
77 CCDC61  
Proximity Label-MS Homo sapiens
78 SDCCAG3  
Proximity Label-MS Homo sapiens
79 CCDC18  
Proximity Label-MS Homo sapiens
80 DNAJC7 7266
Affinity Capture-MS Homo sapiens
81 DDX6 1656
Affinity Capture-MS Homo sapiens
82 ADD1 118
Co-fractionation Homo sapiens
83 MTDH 92140
Affinity Capture-MS Homo sapiens
84 ZWINT  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
85 USP36  
Affinity Capture-MS Homo sapiens
86 TTLL5  
Proximity Label-MS Homo sapiens
87 CEP63  
Proximity Label-MS Homo sapiens
88 CEP131 22994
Proximity Label-MS Homo sapiens
89 AMOT  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
90 HAUS4  
Proximity Label-MS Homo sapiens
91 TEX9  
Proximity Label-MS Homo sapiens
92 PDCD11 22984
Affinity Capture-MS Homo sapiens
93 KIF14 9928
Proximity Label-MS Homo sapiens
94 IFT20 90410
Two-hybrid Homo sapiens
95 LSM12 124801
Affinity Capture-MS Homo sapiens
96 XPO5 57510
Co-fractionation Homo sapiens
97 GTF2I 2969
Co-fractionation Homo sapiens
98 HEXIM1 10614
Affinity Capture-MS Homo sapiens
99 AURKA 6790
Biochemical Activity Homo sapiens
100 KIAA1217 56243
Proximity Label-MS Homo sapiens
101 AMOTL2 51421
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
102 PCNT  
Proximity Label-MS Homo sapiens
103 CEP350 9857
Proximity Label-MS Homo sapiens
104 CENPH  
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
105 SPATA24  
Proximity Label-MS Homo sapiens
106 CEP55 55165
Proximity Label-MS Homo sapiens
107 KRT39  
Affinity Capture-MS Homo sapiens
108 PPIL4  
Affinity Capture-MS Homo sapiens
109 LUZP1 7798
Proximity Label-MS Homo sapiens
110 PGAM5 192111
Proximity Label-MS Homo sapiens
111 SFR1 119392
Two-hybrid Homo sapiens
112 CEP192 55125
Proximity Label-MS Homo sapiens
113 FAM9C  
Affinity Capture-MS Homo sapiens
114 NONO 4841
Affinity Capture-MS Homo sapiens
115 ICE1  
Proximity Label-MS Homo sapiens
116 WRAP73 49856
Proximity Label-MS Homo sapiens
117 EXOC4 60412
Proximity Label-MS Homo sapiens
118 ATF4  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
119 OFD1 8481
Proximity Label-MS Homo sapiens
120 CEP72  
Proximity Label-MS Homo sapiens
121 GCC2 9648
Proximity Label-MS Homo sapiens
122 CCNB1 891
Co-fractionation Homo sapiens
123 TXLNG  
Proximity Label-MS Homo sapiens
124 CEP152  
Proximity Label-MS Homo sapiens
125 FAM118B  
Co-fractionation Homo sapiens
126 VCL 7414
Proximity Label-MS Homo sapiens
127 HAUS8  
Proximity Label-MS Homo sapiens
128 WDYHV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
129 RBM27  
Affinity Capture-MS Homo sapiens
130 NTRK1 4914
Affinity Capture-MS Homo sapiens
131 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 TCHP  
Proximity Label-MS Homo sapiens
133 OPA1 4976
Proximity Label-MS Homo sapiens
134 PLK1 5347
Co-fractionation Homo sapiens
135 DCTN1 1639
Proximity Label-MS Homo sapiens
136 CEP164  
Proximity Label-MS Homo sapiens
137 NSRP1  
Affinity Capture-MS Homo sapiens
138 BICD2 23299
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
139 SPAG5 10615
Proximity Label-MS Homo sapiens
140 CCDC101  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
141 SMC1A 8243
Co-fractionation Homo sapiens
142 AAMDC  
Affinity Capture-MS Homo sapiens
143 CEP85L  
Proximity Label-MS Homo sapiens
144 Bub1  
Affinity Capture-MS Mus musculus
145 ANKRD26 22852
Proximity Label-MS Homo sapiens
146 HAUS7  
Proximity Label-MS Homo sapiens
147 IFT57 55081
Proximity Label-MS Homo sapiens
148 ERC1 23085
Proximity Label-MS Homo sapiens
149 CCDC85C  
Proximity Label-MS Homo sapiens
150 DSN1 79980
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 RGPD6  
Proximity Label-MS Homo sapiens
152 WDR46  
Affinity Capture-MS Homo sapiens
153 WDR82 80335
Affinity Capture-MS Homo sapiens
154 RUFY4  
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
155 ANGPT4  
Affinity Capture-MS Homo sapiens
156 CKAP5 9793
Reconstituted Complex Homo sapiens
157 TRPM7 54822
Proximity Label-MS Homo sapiens
158 FAM170A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
159 ESCO2  
Affinity Capture-MS Homo sapiens
160 Zwint  
Affinity Capture-MS Mus musculus
161 SOGA1 140710
Proximity Label-MS Homo sapiens
162 CEP135  
Proximity Label-MS Homo sapiens
163 CNTLN 54875
Proximity Label-MS Homo sapiens
164 ODF2 4957
Proximity Label-MS Homo sapiens
165 CCDC57 284001
Proximity Label-MS Homo sapiens
166 CAMSAP3  
Proximity Label-MS Homo sapiens
167 AKAP9 10142
Proximity Label-MS Homo sapiens
168 C11orf49  
Proximity Label-MS Homo sapiens
169 ASPM 259266
Proximity Label-MS Homo sapiens
170 SPC24 147841
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
171 CCP110  
Proximity Label-MS Homo sapiens
172 CTTN 2017
Affinity Capture-MS Homo sapiens
173 EGFR 1956
Negative Genetic Homo sapiens
174 NHSL1  
Proximity Label-MS Homo sapiens
175 KIAA1328  
Proximity Label-MS Homo sapiens
176 CCDC66  
Proximity Label-MS Homo sapiens
177 KIAA1524 57650
Two-hybrid Homo sapiens
178 C2CD3  
Proximity Label-MS Homo sapiens
179 CCDC22 28952
Proximity Label-MS Homo sapiens
180 HIP1R 9026
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
181 SART1 9092
Affinity Capture-MS Homo sapiens
182 MIA3 375056
Proximity Label-MS Homo sapiens
183 NXF1 10482
Affinity Capture-RNA Homo sapiens
184 AURKB 9212
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
185 MLF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
186 PHLDB2 90102
Proximity Label-MS Homo sapiens
187 MAPRE3  
Proximity Label-MS Homo sapiens
188 BIRC6 57448
Proximity Label-MS Homo sapiens
189 LRRC49  
Proximity Label-MS Homo sapiens
190 TPST2 8459
Affinity Capture-MS Homo sapiens
191 MFAP1  
Two-hybrid Homo sapiens
192 RPGRIP1L 23322
Proximity Label-MS Homo sapiens
193 PSPC1 55269
Affinity Capture-MS Homo sapiens
194 NSUN2 54888
Co-fractionation Homo sapiens
195 NIN 51199
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
196 CNTROB  
Proximity Label-MS Homo sapiens
197 CEP170B  
Proximity Label-MS Homo sapiens
198 Mis12  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
199 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
200 PLS1 5357
Co-fractionation Homo sapiens
201 FOCAD 54914
Affinity Capture-MS Homo sapiens
202 TOP1 7150
Affinity Capture-MS Homo sapiens
203 NDC80 10403
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
204 DNAJC3 5611
Co-fractionation Homo sapiens
205 RPL23A 6147
Affinity Capture-MS Homo sapiens
206 Bub1b 12236
Affinity Capture-MS Mus musculus
207 KIAA1671  
Proximity Label-MS Homo sapiens
208 USHBP1  
Two-hybrid Homo sapiens
209 PCM1 5108
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
210 HAX1  
Proximity Label-MS Homo sapiens
211 UACA 55075
Proximity Label-MS Homo sapiens
212 KRT19 3880
Proximity Label-MS Homo sapiens
213 ROCK1 6093
Co-fractionation Homo sapiens
214 MAP7D2 256714
Proximity Label-MS Homo sapiens
215 OSBPL1A 114876
Proximity Label-MS Homo sapiens
216 HAUS3  
Proximity Label-MS Homo sapiens
217 ACBD5 91452
Proximity Label-MS Homo sapiens
218 MPHOSPH9  
Proximity Label-MS Homo sapiens
219 KIF15 56992
Proximity Label-MS Homo sapiens
220 TXLNA 200081
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Proximity Label-MS Homo sapiens
221 HERC1 8925
Proximity Label-MS Homo sapiens
222 DZIP3  
Proximity Label-MS Homo sapiens
223 NUDT21 11051
Affinity Capture-MS Homo sapiens
224 MYCBP 26292