Gene description for AMPD2
Gene name adenosine monophosphate deaminase 2
Gene symbol AMPD2
Other names/aliases PCH9
SPG63
Species Homo sapiens
 Database cross references - AMPD2
ExoCarta ExoCarta_271
Vesiclepedia VP_271
Entrez Gene 271
HGNC 469
MIM 102771
UniProt Q01433  
 AMPD2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for AMPD2
Molecular Function
    AMP deaminase activity GO:0003876 IBA
    AMP deaminase activity GO:0003876 IGI
    AMP deaminase activity GO:0003876 NAS
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    IMP biosynthetic process GO:0006188 IBA
    IMP biosynthetic process GO:0006188 IGI
    IMP salvage GO:0032264 IEA
    cholesterol homeostasis GO:0042632 IEA
    AMP metabolic process GO:0046033 IBA
    ATP metabolic process GO:0046034 IEA
    GTP metabolic process GO:0046039 IEA
    cyclic purine nucleotide metabolic process GO:0052652 IMP
    podocyte development GO:0072015 IEA
    energy homeostasis GO:0097009 IGI
Subcellular Localization
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified AMPD2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for AMPD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 EIF1AD 84285
Affinity Capture-MS Homo sapiens
4 ANGEL2 90806
Affinity Capture-MS Homo sapiens
5 C14orf105  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 KRTAP6-1  
Affinity Capture-MS Homo sapiens
7 CSNK2B 1460
Co-fractionation Homo sapiens
8 NEDD4 4734
Reconstituted Complex Homo sapiens
9 ACLY 47
Co-fractionation Homo sapiens
10 POT1  
Two-hybrid Homo sapiens
11 GPR156  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 SDC1 6382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 TUBG1 7283
Affinity Capture-MS Homo sapiens
14 YWHAB 7529
Affinity Capture-MS Homo sapiens
15 INPPL1 3636
Co-fractionation Homo sapiens
16 PDIA4 9601
Co-fractionation Homo sapiens
17 KRTAP19-6  
Affinity Capture-MS Homo sapiens
18 CRYAB 1410
Affinity Capture-MS Homo sapiens
19 DDX17 10521
Affinity Capture-MS Homo sapiens
20 SDC4 6385
Affinity Capture-MS Homo sapiens
21 CD6 923
Affinity Capture-MS Homo sapiens
22 RAB3IL1 5866
Affinity Capture-MS Homo sapiens
23 PSMG2 56984
Affinity Capture-MS Homo sapiens
24 AMPD3 272
Affinity Capture-MS Homo sapiens
25 IDE 3416
Affinity Capture-MS Homo sapiens
26 BRD2  
Affinity Capture-MS Homo sapiens
27 EPS8 2059
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 CCNDBP1 23582
Two-hybrid Homo sapiens
29 TROVE2 6738
Co-fractionation Homo sapiens
30 PARK2  
Affinity Capture-MS Homo sapiens
31 CTBP1 1487
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 SHMT1 6470
Affinity Capture-MS Homo sapiens
33 DDX58 23586
Affinity Capture-RNA Homo sapiens
34 TNPO2 30000
Co-fractionation Homo sapiens
35 SMEK1 55671
Co-fractionation Homo sapiens
36 SDC2 6383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 TUBGCP4 27229
Affinity Capture-MS Homo sapiens
38 KRTAP19-7  
Affinity Capture-MS Homo sapiens
39 PRPS2 5634
Affinity Capture-MS Homo sapiens
40 DEK 7913
Co-fractionation Homo sapiens
41 DCAF8 50717
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 SF3B1 23451
Co-fractionation Homo sapiens
43 LZTS2 84445
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
44 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
45 ARL6 84100
Affinity Capture-MS Homo sapiens
46 TERF1 7013
Two-hybrid Homo sapiens
47 RNPS1 10921
Affinity Capture-MS Homo sapiens
48 CHMP4B 128866
Affinity Capture-MS Homo sapiens
49 AMPD2 271
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
50 RPA2 6118
Proximity Label-MS Homo sapiens
51 WDYHV1  
Two-hybrid Homo sapiens
52 NTRK1 4914
Affinity Capture-MS Homo sapiens
53 NME7 29922
Affinity Capture-MS Homo sapiens
54 IL7R  
Affinity Capture-MS Homo sapiens
55 HIRIP3  
Affinity Capture-MS Homo sapiens
56 MLX  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 DNAJA1 3301
Co-fractionation Homo sapiens
58 FAM136A 84908
Affinity Capture-MS Homo sapiens
59 ZMPSTE24 10269
Affinity Capture-MS Homo sapiens
60 PRC1 9055
Affinity Capture-MS Homo sapiens
61 CD44 960
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
62 YWHAG 7532
Affinity Capture-MS Homo sapiens
63 PSMA5 5686
Affinity Capture-MS Homo sapiens
64 PITRM1 10531
Affinity Capture-MS Homo sapiens
65 PHF8  
Affinity Capture-MS Homo sapiens
66 TBC1D9B 23061
Co-fractionation Homo sapiens
67 TCHP  
Affinity Capture-MS Homo sapiens
68 GPBP1L1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 XIAP  
Affinity Capture-MS Homo sapiens
70 PPP4R2  
Co-fractionation Homo sapiens
71 TULP3 7289
Affinity Capture-MS Homo sapiens
72 PAGE1  
Affinity Capture-MS Homo sapiens
73 AMPD1  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which AMPD2 is involved
PathwayEvidenceSource
Metabolism TAS Reactome
Metabolism of nucleotides TAS Reactome
Nucleotide salvage TAS Reactome
Purine salvage TAS Reactome





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