Gene description for PLD2
Gene name phospholipase D2
Gene symbol PLD2
Other names/aliases -
Species Homo sapiens
 Database cross references - PLD2
ExoCarta ExoCarta_5338
Entrez Gene 5338
HGNC 9068
MIM 602384
UniProt O14939  
 PLD2 identified in exosomes derived from the following tissue/cell type
Hepatocytes 26054723    
 Gene ontology annotations for PLD2
Molecular Function
    phosphatidylinositol binding GO:0035091 IEA
    protein binding GO:0005515 IPI
    N-acylphosphatidylethanolamine-specific phospholipase D activity GO:0070290 IEA
    phospholipase D activity GO:0004630 IEA
Biological Process
    phosphatidylglycerol biosynthetic process GO:0006655 TAS
    inositol lipid-mediated signaling GO:0048017 IEA
    regulation of cell migration GO:0030334 IEA
    glycerophospholipid biosynthetic process GO:0046474 TAS
    regulation of phagocytosis GO:0050764 IEA
    cytoskeleton organization GO:0007010 TAS
    synaptic vesicle recycling GO:0036465 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    innate immune response GO:0045087 TAS
    phosphatidic acid biosynthetic process GO:0006654 TAS
    lipid catabolic process GO:0016042 IEA
    G-protein coupled receptor internalization GO:0002031 IEA
    small molecule metabolic process GO:0044281 TAS
    phospholipid metabolic process GO:0006644 TAS
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
Subcellular Localization
    brush border membrane GO:0031526 IEA
    endoplasmic reticulum membrane GO:0005789 TAS
    plasma membrane GO:0005886 TAS
 Experiment description of studies that identified PLD2 in exosomes
1
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for PLD2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PIP5K1A 8394
Invivo Homo sapiens
Invitro Homo sapiens
2 CAV2 858
Co-fractionation Homo sapiens
3 OPRM1 4988
Invivo Homo sapiens
Invitro Homo sapiens
4 SRC 6714
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
5 Actb 81822
Reconstituted Complex Rattus norvegicus
6 RPTOR 57521
Affinity Capture-Western Homo sapiens
7 ALDOC 230
Invitro Homo sapiens
8 GAPDH 2597
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
9 PEA15  
Affinity Capture-Western Homo sapiens
10 PLCG1 5335
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
11 EGFR 1956
Affinity Capture-Western Homo sapiens
12 GM2A 2760
Invitro Homo sapiens
13 PRKCD 5580
Invivo Homo sapiens
Invitro Homo sapiens
14 BIN1 274
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
15 CAV1 857
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
16 AQP3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 ALDOA 226
Invivo Homo sapiens
Invitro Homo sapiens
18 MTOR 2475
Affinity Capture-Western Homo sapiens
19 AMPH  
Reconstituted Complex Homo sapiens
Phenotypic Suppression Homo sapiens
Affinity Capture-Western Homo sapiens
20 ARF1 375
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
21 PRKCA 5578
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which PLD2 is involved
PathwayEvidenceSource
Role of phospholipids in phagocytosis TAS Reactome
Synthesis of PA TAS Reactome
Synthesis of PG TAS Reactome





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