Gene description for NT5C2
Gene name 5'-nucleotidase, cytosolic II
Gene symbol NT5C2
Other names/aliases GMP
NT5B
PNT5
SPG45
SPG65
cN-II
Species Homo sapiens
 Database cross references - NT5C2
ExoCarta ExoCarta_22978
Entrez Gene 22978
HGNC 8022
MIM 600417
UniProt P49902  
 NT5C2 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for NT5C2
Molecular Function
    protein binding GO:0005515 IPI
    nucleoside phosphotransferase activity GO:0050146 TAS
    nucleotide binding GO:0000166 IEA
    5'-nucleotidase activity GO:0008253 EXP
    metal ion binding GO:0046872 IEA
Biological Process
    phosphorylation GO:0016310 TAS
    IMP metabolic process GO:0046040 IBA
    dephosphorylation GO:0016311 TAS
    nucleobase-containing small molecule metabolic process GO:0055086 TAS
    purine nucleobase metabolic process GO:0006144 TAS
    adenosine metabolic process GO:0046085 IBA
    small molecule metabolic process GO:0044281 TAS
    drug metabolic process GO:0017144 TAS
    purine nucleotide catabolic process GO:0006195 TAS
Subcellular Localization
    cytosol GO:0005829 TAS
 Experiment description of studies that identified NT5C2 in exosomes
1
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NT5C2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FLJ22494  
Two-hybrid Homo sapiens
2 NT5C2 22978
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
3 MOBKL2B  
Two-hybrid Homo sapiens
4 MGC3162  
Two-hybrid Homo sapiens
5 FXR2 9513
Two-hybrid Homo sapiens
6 NME7  
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which NT5C2 is involved
PathwayEvidenceSource
Abacavir metabolism TAS Reactome
Purine catabolism TAS Reactome





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