Gene description for HSPBP1
Gene name HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
Gene symbol HSPBP1
Other names/aliases FES1
Species Homo sapiens
 Database cross references - HSPBP1
ExoCarta ExoCarta_23640
Vesiclepedia VP_23640
Entrez Gene 23640
HGNC 24989
MIM 612939
UniProt Q9NZL4  
 HSPBP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HSPBP1
Molecular Function
    adenyl-nucleotide exchange factor activity GO:0000774 IBA
    enzyme inhibitor activity GO:0004857 TAS
    protein binding GO:0005515 IPI
    ubiquitin protein ligase binding GO:0031625 IPI
    molecular sequestering activity GO:0140313 IDA
Biological Process
    protein folding GO:0006457 TAS
    positive regulation of protein ubiquitination GO:0031398 IDA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IDA
Subcellular Localization
    extracellular space GO:0005615 IDA
    endoplasmic reticulum GO:0005783 IBA
 Experiment description of studies that identified HSPBP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
9
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HSPBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FAN1  
Affinity Capture-MS Homo sapiens
2 UBE2H 7328
Affinity Capture-MS Homo sapiens
3 ACTR2 10097
Co-fractionation Homo sapiens
Co-fractionation Homo sapiens
4 ABL2  
Affinity Capture-MS Homo sapiens
5 KRT27 342574
Two-hybrid Homo sapiens
6 GMNN  
Affinity Capture-MS Homo sapiens
7 ELF1 1997
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 DPH2 1802
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 HMCES  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 HSPA6 3310
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 RPS4Y1 6192
Affinity Capture-MS Homo sapiens
12 RIN3  
Affinity Capture-MS Homo sapiens
13 ZPR1 8882
Co-fractionation Homo sapiens
14 CRK 1398
Affinity Capture-MS Homo sapiens
15 ZNF703  
Affinity Capture-MS Homo sapiens
16 C14orf142  
Co-fractionation Homo sapiens
17 GAK 2580
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 HSPA1L 3305
Affinity Capture-MS Homo sapiens
19 TAB1 10454
Affinity Capture-MS Homo sapiens
20 APP 351
Reconstituted Complex Homo sapiens
21 HSF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 SURF2  
Co-fractionation Homo sapiens
23 HSPA2 3306
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 Hspa8 24468
Affinity Capture-Western Rattus norvegicus
25 PTPN23 25930
Affinity Capture-MS Homo sapiens
26 METAP2 10988
Affinity Capture-MS Homo sapiens
27 HGS 9146
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PPP1R7 5510
Co-fractionation Homo sapiens
29 SF3A2 8175
Affinity Capture-MS Homo sapiens
30 MKLN1  
Affinity Capture-MS Homo sapiens
31 LPP 4026
Co-fractionation Homo sapiens
32 C6orf106  
Affinity Capture-MS Homo sapiens
33 TOM1L1 10040
Co-fractionation Homo sapiens
34 LRSAM1 90678
Co-fractionation Homo sapiens
35 P3H1 64175
Co-fractionation Homo sapiens
36 GPN1  
Co-fractionation Homo sapiens
37 MAVS 57506
Affinity Capture-MS Homo sapiens
38 FKBP10 60681
Co-fractionation Homo sapiens
39 LRIF1  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
40 HSPA8 3312
Two-hybrid Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 GARS 2617
Co-fractionation Homo sapiens
42 BAG5 9529
Affinity Capture-MS Homo sapiens
43 FOXC1  
Affinity Capture-MS Homo sapiens
44 DBNL 28988
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
45 ERO1L 30001
Co-fractionation Homo sapiens
46 AGFG1 3267
Co-fractionation Homo sapiens
47 RUFY1 80230
Affinity Capture-MS Homo sapiens
48 ANXA11 311
Affinity Capture-MS Homo sapiens
49 EIF2A 83939
Affinity Capture-MS Homo sapiens
50 CLTCL1 8218
Affinity Capture-MS Homo sapiens
51 TBC1D32 221322
Proximity Label-MS Homo sapiens
52 VCL 7414
Affinity Capture-MS Homo sapiens
53 RPA2 6118
Proximity Label-MS Homo sapiens
54 NTRK1 4914
Affinity Capture-MS Homo sapiens
55 METTL21A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 DNAJA1 3301
Proximity Label-MS Homo sapiens
57 ARMC1 55156
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 TPRKB 51002
Co-fractionation Homo sapiens
59 QRICH1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 GTF3C5 9328
Affinity Capture-MS Homo sapiens
61 DRAP1 10589
Co-fractionation Homo sapiens
62 DNAJC13 23317
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
63 SF3A3 10946
Affinity Capture-MS Homo sapiens
64 OGT 8473
Co-fractionation Homo sapiens
65 DNAJC7 7266
Proximity Label-MS Homo sapiens
66 CBFB 865
Affinity Capture-MS Homo sapiens
67 NOTCH2NL 388677
Two-hybrid Homo sapiens
68 ALAD 210
Co-fractionation Homo sapiens
69 HSPA5 3309
Reconstituted Complex Homo sapiens
70 CSTF1 1477
Co-fractionation Homo sapiens
71 SF1 7536
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 AMBRA1  
Affinity Capture-MS Homo sapiens
73 UBAP2 55833
Affinity Capture-MS Homo sapiens
74 CUL3 8452
Affinity Capture-MS Homo sapiens
75 FKBP8 23770
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
76 MAP3K7  
Affinity Capture-MS Homo sapiens
77 C21orf59 56683
Affinity Capture-MS Homo sapiens
78 G6PD 2539
Co-fractionation Homo sapiens
79 NT5C2 22978
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
80 CDC25C  
Affinity Capture-MS Homo sapiens
81 RPA3 6119
Proximity Label-MS Homo sapiens
82 PGLYRP1 8993
Reconstituted Complex Homo sapiens
Co-fractionation Homo sapiens
Co-purification Homo sapiens
83 HSPA1A 3303
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-Luminescence Homo sapiens
84 HSPA4 3308
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
85 ESRP2 80004
Affinity Capture-MS Homo sapiens
86 NMD3 51068
Co-fractionation Homo sapiens
87 DDRGK1 65992
Affinity Capture-MS Homo sapiens
88 HSPA9 3313
Reconstituted Complex Homo sapiens
89 FOXK1 221937
Affinity Capture-MS Homo sapiens
90 UBXN2B  
Affinity Capture-MS Homo sapiens
91 PPME1 51400
Co-fractionation Homo sapiens
92 TACC3  
Affinity Capture-MS Homo sapiens
93 STUB1 10273
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
94 MAP2K2 5605
Affinity Capture-MS Homo sapiens
95 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
96 FASN 2194
Co-fractionation Homo sapiens
97 ARFIP1 27236
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 TTC1 7265
Affinity Capture-MS Homo sapiens
99 WDR4 10785
Co-fractionation Homo sapiens
100 FBXL6  
Affinity Capture-MS Homo sapiens
101 POTEF 728378
Affinity Capture-MS Homo sapiens
102 PRNP 5621
Affinity Capture-MS Homo sapiens
103 ASNS 440
Co-fractionation Homo sapiens
104 PPP1R13L 10848
Affinity Capture-MS Homo sapiens
105 FLYWCH2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 CCDC117  
Affinity Capture-MS Homo sapiens
107 QSER1  
Affinity Capture-MS Homo sapiens
108 PGAM2 5224
Affinity Capture-MS Homo sapiens
109 NUP50 10762
Co-fractionation Homo sapiens
110 HNRNPL 3191
Co-fractionation Homo sapiens
111 NEDD1 121441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 SH3GLB1 51100
Co-fractionation Homo sapiens
113 HSF1  
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 PUM1 9698
Affinity Capture-MS Homo sapiens
115 METTL3  
Affinity Capture-MS Homo sapiens
116 ZNF503 84858
Affinity Capture-MS Homo sapiens
117 ELF2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
118 SNW1 22938
Affinity Capture-MS Homo sapiens
119 DGCR14  
Affinity Capture-MS Homo sapiens
120 KBTBD4  
Affinity Capture-MS Homo sapiens
121 VIM 7431
Affinity Capture-MS Homo sapiens
122 STAM 8027
Affinity Capture-MS Homo sapiens
123 HSPA1B 3304
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
124 EDRF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
125 EFEMP2 30008
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which HSPBP1 is involved
No pathways found





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