Gene description for HKDC1
Gene name hexokinase domain containing 1
Gene symbol HKDC1
Other names/aliases -
Species Homo sapiens
 Database cross references - HKDC1
ExoCarta ExoCarta_80201
Vesiclepedia VP_80201
Entrez Gene 80201
HGNC 23302
UniProt Q2TB90  
 HKDC1 identified in sEVs derived from the following tissue/cell type
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for HKDC1
Molecular Function
    glucokinase activity GO:0004340 IBA
    glucokinase activity GO:0004340 IDA
    ATP binding GO:0005524 IEA
    D-glucose binding GO:0005536 IEA
    fructokinase activity GO:0008865 IBA
Biological Process
    intracellular glucose homeostasis GO:0001678 IBA
    intracellular glucose homeostasis GO:0001678 IDA
    glucose metabolic process GO:0006006 IBA
    glycolytic process GO:0006096 IBA
    glycolytic process GO:0006096 IEA
    carbohydrate phosphorylation GO:0046835 IEA
    glucose 6-phosphate metabolic process GO:0051156 IBA
    glucose 6-phosphate metabolic process GO:0051156 IDA
Subcellular Localization
    photoreceptor inner segment GO:0001917 IEA
    cytoplasm GO:0005737 IDA
    mitochondrion GO:0005739 IBA
    mitochondrion GO:0005739 IDA
    cytosol GO:0005829 IBA
    mitochondrial membrane GO:0031966 IEA
 Experiment description of studies that identified HKDC1 in sEVs
1
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for HKDC1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 DHDH  
Affinity Capture-MS Homo sapiens
3 SALL2  
Affinity Capture-MS Homo sapiens
4 CA6 765
Affinity Capture-MS Homo sapiens
5 MAPK4  
Affinity Capture-MS Homo sapiens
6 WFDC11  
Affinity Capture-MS Homo sapiens
7 CA10  
Affinity Capture-MS Homo sapiens
8 HK1 3098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
9 CST5 1473
Affinity Capture-MS Homo sapiens
10 UCN3  
Affinity Capture-MS Homo sapiens
11 LDLRAD1  
Affinity Capture-MS Homo sapiens
12 RAMP3  
Affinity Capture-MS Homo sapiens
13 BSG 682
Affinity Capture-MS Homo sapiens
14 HK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CD83  
Affinity Capture-MS Homo sapiens
16 VSTM1  
Affinity Capture-MS Homo sapiens
17 KLRB1  
Affinity Capture-MS Homo sapiens
18 SNX21  
Affinity Capture-MS Homo sapiens
19 SDC2 6383
Affinity Capture-MS Homo sapiens
20 ADAM7  
Affinity Capture-MS Homo sapiens
21 LYZL6  
Affinity Capture-MS Homo sapiens
22 CD3G 917
Affinity Capture-MS Homo sapiens
23 MCFD2 90411
Affinity Capture-MS Homo sapiens
24 RPS27 6232
Affinity Capture-MS Homo sapiens
25 LSM7  
Affinity Capture-MS Homo sapiens
26 TMEM25  
Affinity Capture-MS Homo sapiens
27 PBXIP1 57326
Affinity Capture-MS Homo sapiens
28 KIF23 9493
Affinity Capture-MS Homo sapiens
29 DNAJA1 3301
Affinity Capture-MS Homo sapiens
30 PPP2R2B 5521
Affinity Capture-MS Homo sapiens
31 NOS2  
Affinity Capture-MS Homo sapiens
32 CST11  
Affinity Capture-MS Homo sapiens
33 GML  
Affinity Capture-MS Homo sapiens
34 KRAS 3845
Synthetic Growth Defect Homo sapiens
35 OXCT2  
Affinity Capture-MS Homo sapiens
36 CUL4A 8451
Affinity Capture-MS Homo sapiens
37 SPRYD7 57213
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which HKDC1 is involved
PathwayEvidenceSource
Glucose metabolism TAS Reactome
Glycolysis TAS Reactome
Metabolism TAS Reactome
Metabolism of carbohydrates TAS Reactome





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