Gene description for GSPT2
Gene name G1 to S phase transition 2
Gene symbol GSPT2
Other names/aliases ERF3B
GST2
Species Homo sapiens
 Database cross references - GSPT2
ExoCarta ExoCarta_23708
Vesiclepedia VP_23708
Entrez Gene 23708
HGNC 4622
MIM 300418
UniProt Q8IYD1  
 GSPT2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for GSPT2
Molecular Function
    RNA binding GO:0003723 HDA
    translation release factor activity GO:0003747 IBA
    translation release factor activity GO:0003747 IDA
    GTPase activity GO:0003924 IBA
    GTPase activity GO:0003924 IDA
    protein binding GO:0005515 IPI
    GTP binding GO:0005525 IEA
Biological Process
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IEA
    translation GO:0006412 IBA
    translational termination GO:0006415 IDA
Subcellular Localization
    cytosol GO:0005829 TAS
    translation release factor complex GO:0018444 IBA
 Experiment description of studies that identified GSPT2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GSPT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 RALY 22913
Affinity Capture-MS Homo sapiens
3 HNRNPC 3183
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 RPL26L1 51121
Affinity Capture-MS Homo sapiens
5 NCBP1 4686
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 BCLAF1 9774
Affinity Capture-MS Homo sapiens
7 ATP6V1C1 528
Co-fractionation Homo sapiens
8 MKRN3  
Affinity Capture-MS Homo sapiens
9 LARP1B 55132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 ZFR 51663
Affinity Capture-MS Homo sapiens
11 PUM2 23369
Affinity Capture-MS Homo sapiens
12 APP 351
Reconstituted Complex Homo sapiens
13 IGF2BP3 10643
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 RNPS1 10921
Affinity Capture-MS Homo sapiens
15 PABPC4L 132430
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SRRM2 23524
Affinity Capture-MS Homo sapiens
17 GSPT1 2935
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 TRA2A 29896
Affinity Capture-MS Homo sapiens
19 ZCCHC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 STAU2 27067
Affinity Capture-MS Homo sapiens
21 ATG7 10533
Co-fractionation Homo sapiens
22 MYCN  
Affinity Capture-MS Homo sapiens
23 ADSS 159
Affinity Capture-MS Homo sapiens
24 PABPC4 8761
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 FAM120A 23196
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 DHX36 170506
Affinity Capture-MS Homo sapiens
27 ATXN2 6311
Affinity Capture-MS Homo sapiens
28 CCT2 10576
Co-fractionation Homo sapiens
29 CLK2 1196
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PIP4K2C 79837
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 SHMT1 6470
Co-fractionation Homo sapiens
32 C17orf85  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 STAU1 6780
Affinity Capture-MS Homo sapiens
34 UBR5 51366
Affinity Capture-Western Homo sapiens
35 PABPC1 26986
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
37 MAGOH 4116
Affinity Capture-MS Homo sapiens
38 PAPSS2 9060
Co-fractionation Homo sapiens
39 U2SURP 23350
Affinity Capture-MS Homo sapiens
40 LARP1 23367
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ZNF326 284695
Affinity Capture-MS Homo sapiens
42 SNX1 6642
Co-fractionation Homo sapiens
43 RBBP6 5930
Affinity Capture-MS Homo sapiens
44 NUAK2  
Affinity Capture-MS Homo sapiens
45 NASP 4678
Co-fractionation Homo sapiens
46 TRIM31  
Affinity Capture-MS Homo sapiens
47 CLK3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PNN 5411
Affinity Capture-MS Homo sapiens
49 SRSF1 6426
Affinity Capture-MS Homo sapiens
50 PUM1 9698
Affinity Capture-MS Homo sapiens
51 CTTN 2017
Co-fractionation Homo sapiens
52 ETF1 2107
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 DHX30 22907
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 ZC3H11A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 APOBEC3B 9582
Affinity Capture-MS Homo sapiens
56 YTHDC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 STAT1 6772
Co-fractionation Homo sapiens
58 APOBEC3F 200316
Affinity Capture-MS Homo sapiens
59 MYC  
Affinity Capture-MS Homo sapiens
60 ZC3H18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 RBM14-RBM4 100526737
Affinity Capture-MS Homo sapiens
62 ZDHHC18 84243
Affinity Capture-MS Homo sapiens
63 SRSF10 10772
Affinity Capture-MS Homo sapiens
64 DDRGK1 65992
Affinity Capture-MS Homo sapiens
65 PDE1C 5137
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 LARP4 113251
Affinity Capture-MS Homo sapiens
67 RBMS2 5939
Affinity Capture-MS Homo sapiens
68 PAIP1 10605
Affinity Capture-MS Homo sapiens
69 YBX1 4904
Affinity Capture-MS Homo sapiens
70 MKRN1 23608
Affinity Capture-MS Homo sapiens
71 RPL6 6128
Cross-Linking-MS (XL-MS) Homo sapiens
72 IREB2 3658
Affinity Capture-MS Homo sapiens
73 SRSF12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
74 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
75 SQSTM1 8878
Proximity Label-MS Homo sapiens
76 THRAP3 9967
Affinity Capture-MS Homo sapiens
77 ELAVL2 1993
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
78 ZDHHC23  
Affinity Capture-MS Homo sapiens
79 SHMT2 6472
Co-fractionation Homo sapiens
80 TRA2B 6434
Affinity Capture-MS Homo sapiens
81 SUGP2  
Affinity Capture-MS Homo sapiens
82 UFL1 23376
Affinity Capture-MS Homo sapiens
83 IDE 3416
Co-fractionation Homo sapiens
84 Krr1  
Two-hybrid Mus musculus
85 RPL38 6169
Cross-Linking-MS (XL-MS) Homo sapiens
86 MOV10 4343
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
87 YBX3 8531
Affinity Capture-MS Homo sapiens
88 UPF1 5976
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 RDX 5962
Co-fractionation Homo sapiens
90 RPS6 6194
Cross-Linking-MS (XL-MS) Homo sapiens
91 PABPC1L 80336
Affinity Capture-MS Homo sapiens
92 SNX2 6643
Co-fractionation Homo sapiens
93 KIF1C 10749
Affinity Capture-MS Homo sapiens
94 IGF2BP2 10644
Affinity Capture-MS Homo sapiens
95 CASC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here