Gene description for TRMT1
Gene name tRNA methyltransferase 1 homolog (S. cerevisiae)
Gene symbol TRMT1
Other names/aliases TRM1
Species Homo sapiens
 Database cross references - TRMT1
ExoCarta ExoCarta_55621
Vesiclepedia VP_55621
Entrez Gene 55621
HGNC 25980
MIM 611669
UniProt Q9NXH9  
 TRMT1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for TRMT1
Molecular Function
    tRNA binding GO:0000049 IEA
    RNA binding GO:0003723 HDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
    tRNA (guanine(26)-N2)-dimethyltransferase activity GO:0160104 EXP
Biological Process
    tRNA N2-guanine methylation GO:0002940 IBA
    tRNA modification GO:0006400 TAS
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 TAS
    mitochondrion GO:0005739 HTP
 Experiment description of studies that identified TRMT1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for TRMT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 DENR 8562
Co-fractionation Homo sapiens
3 DPP9 91039
Affinity Capture-MS Homo sapiens
4 QARS 5859
Co-fractionation Homo sapiens
5 ATP6V1B2 526
Co-fractionation Homo sapiens
6 P4HA2 8974
Affinity Capture-MS Homo sapiens
7 RSAD1  
Affinity Capture-MS Homo sapiens
8 KARS 3735
Co-fractionation Homo sapiens
9 SEPT2 4735
Co-fractionation Homo sapiens
10 DNAJB6 10049
Affinity Capture-MS Homo sapiens
11 APEX1 328
Affinity Capture-RNA Homo sapiens
12 RFPL4B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 HDAC3 8841
Affinity Capture-MS Homo sapiens
14 DARS 1615
Co-fractionation Homo sapiens
15 D2HGDH 728294
Affinity Capture-MS Homo sapiens
16 SENP1  
Affinity Capture-MS Homo sapiens
17 CHCHD2  
Affinity Capture-MS Homo sapiens
18 NARS 4677
Co-fractionation Homo sapiens
19 EXD2  
Proximity Label-MS Homo sapiens
20 ATG7 10533
Affinity Capture-MS Homo sapiens
21 DDX6 1656
Co-fractionation Homo sapiens
22 ZNF598 90850
Co-fractionation Homo sapiens
23 WIF1 11197
Affinity Capture-MS Homo sapiens
24 CTPS1 1503
Co-fractionation Homo sapiens
25 TWF1 5756
Co-fractionation Homo sapiens
26 KIF14 9928
Affinity Capture-MS Homo sapiens
27 SAMHD1 25939
Co-fractionation Homo sapiens
28 AGO2 27161
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
29 RTFDC1  
Co-fractionation Homo sapiens
30 PARK2  
Affinity Capture-MS Homo sapiens
31 TARBP2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
32 ACADVL 37
Co-fractionation Homo sapiens
33 TCEAL1  
Affinity Capture-MS Homo sapiens
34 NUDC 10726
Co-fractionation Homo sapiens
35 WDYHV1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
36 AKAP11 11215
Affinity Capture-MS Homo sapiens
37 HEMK1  
Affinity Capture-MS Homo sapiens
38 TPRKB 51002
Co-fractionation Homo sapiens
39 ATP6V1B1 525
Co-fractionation Homo sapiens
40 IARS 3376
Co-fractionation Homo sapiens
41 LIPG 9388
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 USP4 7375
Affinity Capture-MS Homo sapiens
43 MAGEA11  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
44 CHDH 55349
Affinity Capture-MS Homo sapiens
45 CUL3 8452
Affinity Capture-MS Homo sapiens
46 EPRS 2058
Co-fractionation Homo sapiens
47 LARS 51520
Co-fractionation Homo sapiens
48 THUMPD2  
Co-fractionation Homo sapiens
49 VBP1 7411
Co-fractionation Homo sapiens
50 STAT1 6772
Co-fractionation Homo sapiens
51 RPA3 6119
Proximity Label-MS Homo sapiens
52 SEC31A 22872
Co-fractionation Homo sapiens
53 MRFAP1  
Affinity Capture-MS Homo sapiens
54 HIST1H1A 3024
Affinity Capture-MS Homo sapiens
55 PDPR 55066
Affinity Capture-MS Homo sapiens
56 TXNDC5 81567
Co-fractionation Homo sapiens
57 GANAB 23193
Co-fractionation Homo sapiens
58 CSDE1 7812
Co-fractionation Homo sapiens
59 AARS 16
Co-fractionation Homo sapiens
60 PDP1 54704
Affinity Capture-MS Homo sapiens
61 ZC3H3  
Affinity Capture-MS Homo sapiens
62 PDE3A  
Co-fractionation Homo sapiens
63 XRCC5 7520
Co-fractionation Homo sapiens
64 TUBB2A 7280
Affinity Capture-MS Homo sapiens
65 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
66 FASN 2194
Positive Genetic Homo sapiens
67 CLPX 10845
Affinity Capture-MS Homo sapiens
68 JUP 3728
Co-fractionation Homo sapiens
69 LIPH 200879
Affinity Capture-MS Homo sapiens
70 PDHA1 5160
Proximity Label-MS Homo sapiens
71 NDUFA12 55967
Affinity Capture-MS Homo sapiens
72 CAP2 10486
Co-fractionation Homo sapiens
73 COX8A  
Proximity Label-MS Homo sapiens
74 RNASE10  
Affinity Capture-MS Homo sapiens
75 PUF60 22827
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 SSBP1 6742
Proximity Label-MS Homo sapiens
77 SKAP1 8631
Affinity Capture-MS Homo sapiens
78 TERF1 7013
Two-hybrid Homo sapiens
79 EZH2  
Affinity Capture-Western Homo sapiens
80 AARS2  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 EP300 2033
Affinity Capture-MS Homo sapiens
82 C1QBP 708
Co-fractionation Homo sapiens
83 LDLR 3949
Positive Genetic Homo sapiens
84 C9orf72  
Affinity Capture-MS Homo sapiens
85 YBEY  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 NDUFS6  
Affinity Capture-MS Homo sapiens
87 CCRN4L  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TRMT1 is involved
PathwayEvidenceSource
Metabolism of RNA TAS Reactome
tRNA modification in the nucleus and cytosol TAS Reactome
tRNA processing TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here