Gene description for PPP6R1
Gene name protein phosphatase 6, regulatory subunit 1
Gene symbol PPP6R1
Other names/aliases KIAA1115
PP6R1
SAP190
SAPS1
Species Homo sapiens
 Database cross references - PPP6R1
ExoCarta ExoCarta_22870
Vesiclepedia VP_22870
Entrez Gene 22870
HGNC 29195
MIM 610875
UniProt Q9UPN7  
 PPP6R1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for PPP6R1
Molecular Function
    protein binding GO:0005515 IPI
    protein phosphatase regulator activity GO:0019888 IBA
    protein phosphatase regulator activity GO:0019888 IDA
    protein phosphatase binding GO:0019903 IEA
    small GTPase binding GO:0031267 IPI
Biological Process
    regulation of signal transduction GO:0009966 IBA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified PPP6R1 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 234
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-C3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 237
MISEV standards
EM
Biophysical techniques
TSG101|Alix|HSC70|GAPDH
Enriched markers
HSP90B1
Negative markers
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
7
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PPP6R1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 ARHGEF2 9181
Affinity Capture-MS Homo sapiens
3 DNAJC11 55735
Affinity Capture-MS Homo sapiens
4 SRPRB 58477
Affinity Capture-MS Homo sapiens
5 TRIM66  
Affinity Capture-MS Homo sapiens
6 BRCA1 672
Affinity Capture-MS Homo sapiens
7 SRPR 6734
Affinity Capture-MS Homo sapiens
8 FPR1  
Affinity Capture-MS Homo sapiens
9 UBC 7316
Affinity Capture-MS Homo sapiens
10 DNAJC25 548645
Affinity Capture-MS Homo sapiens
11 MED24 9862
Co-fractionation Homo sapiens
12 RPS6KB2  
Affinity Capture-MS Homo sapiens
13 ANKRD28 23243
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 PNKD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 CNOT10 25904
Proximity Label-MS Homo sapiens
16 DYRK1A 1859
Affinity Capture-MS Homo sapiens
17 RAB40C 57799
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
18 CLEC2B  
Affinity Capture-MS Homo sapiens
19 GPR55  
Affinity Capture-MS Homo sapiens
20 PDCD2 5134
Affinity Capture-MS Homo sapiens
21 HADHA 3030
Affinity Capture-MS Homo sapiens
22 ATG16L1 55054
Affinity Capture-MS Homo sapiens
23 HSPA12B 116835
Affinity Capture-MS Homo sapiens
24 PPP6R3 55291
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 HUWE1 10075
Affinity Capture-MS Homo sapiens
26 NPAS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 PFDN6 10471
Proximity Label-MS Homo sapiens
28 AMOT  
Proximity Label-MS Homo sapiens
29 PFDN5 5204
Proximity Label-MS Homo sapiens
30 GPR17 2840
Affinity Capture-MS Homo sapiens
31 MTNR1B  
Two-hybrid Homo sapiens
32 CPA6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 USP10 9100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
34 PPP6R2 9701
Proximity Label-MS Homo sapiens
Proximity Label-MS Homo sapiens
35 P2RY8  
Affinity Capture-MS Homo sapiens
36 Ppp6c 67857
Affinity Capture-MS Mus musculus
37 ILVBL 10994
Affinity Capture-MS Homo sapiens
38 COMTD1 118881
Affinity Capture-MS Homo sapiens
39 PFDN2 5202
Proximity Label-MS Homo sapiens
40 Ankrd28  
Affinity Capture-MS Mus musculus
41 CNOT1 23019
Proximity Label-MS Homo sapiens
42 S1PR1 1901
Affinity Capture-MS Homo sapiens
43 KIAA1683  
Affinity Capture-MS Homo sapiens
44 GPR182  
Affinity Capture-MS Homo sapiens
45 DCTN1 1639
Proximity Label-MS Homo sapiens
46 CDHR4  
Affinity Capture-MS Homo sapiens
47 HUS1B  
Affinity Capture-MS Homo sapiens
48 ACBD5 91452
Proximity Label-MS Homo sapiens
49 TRMT2A 27037
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 ANKRD44 91526
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
51 FAM43A  
Affinity Capture-MS Homo sapiens
52 CDC14A  
Proximity Label-MS Homo sapiens
53 PRG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
54 NTRK1 4914
Affinity Capture-MS Homo sapiens
55 SGPL1 8879
Affinity Capture-MS Homo sapiens
56 HADHB 3032
Affinity Capture-MS Homo sapiens
57 MOB1B 92597
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
58 ABT1 29777
Affinity Capture-MS Homo sapiens
59 FGF14  
Affinity Capture-MS Homo sapiens
60 XPO1 7514
Affinity Capture-MS Homo sapiens
61 DNAJC2 27000
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
62 ACAD11 84129
Affinity Capture-MS Homo sapiens
63 MGARP  
Affinity Capture-MS Homo sapiens
64 CUL3 8452
Affinity Capture-MS Homo sapiens
65 EGFR 1956
Affinity Capture-MS Homo sapiens
66 DCLRE1C  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 FOXL1  
Affinity Capture-MS Homo sapiens
68 PRPSAP1 5635
Affinity Capture-MS Homo sapiens
69 RIPK4  
Affinity Capture-MS Homo sapiens
70 GTF3C4 9329
Affinity Capture-MS Homo sapiens
71 CTSG 1511
Affinity Capture-MS Homo sapiens
72 ANKRD52 283373
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 ACSL3 2181
Affinity Capture-MS Homo sapiens
74 MYO6 4646
Proximity Label-MS Homo sapiens
75 IRF5  
Proximity Label-MS Homo sapiens
76 GPR45  
Affinity Capture-MS Homo sapiens
77 GOLGA1  
Proximity Label-MS Homo sapiens
78 HSPA1A 3303
Affinity Capture-MS Homo sapiens
79 HSPA4 3308
Proximity Label-MS Homo sapiens
80 TTF2 8458
Proximity Label-MS Homo sapiens
81 PPP6C 5537
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 P2RY10  
Affinity Capture-MS Homo sapiens
83 LPPR2  
Affinity Capture-MS Homo sapiens
84 FOXK1 221937
Affinity Capture-MS Homo sapiens
85 SH3GL2  
Two-hybrid Homo sapiens
86 MED14  
Co-fractionation Homo sapiens
87 PRKDC 5591
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
88 DRD2  
Affinity Capture-MS Homo sapiens
89 IKBKAP 8518
Proximity Label-MS Homo sapiens
90 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
91 SLC25A11 8402
Affinity Capture-MS Homo sapiens
92 ALDH3A2 224
Affinity Capture-MS Homo sapiens
93 USP49 25862
Affinity Capture-MS Homo sapiens
94 DNAJC19 131118
Affinity Capture-MS Homo sapiens
95 KNTC1 9735
Proximity Label-MS Homo sapiens
96 AGO1  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
97 EZR 7430
Proximity Label-MS Homo sapiens
98 CHRM4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 DCTN2 10540
Proximity Label-MS Homo sapiens
100 DDX58 23586
Affinity Capture-RNA Homo sapiens
101 CHRM3 1131
Affinity Capture-MS Homo sapiens
102 TBK1 29110
Affinity Capture-MS Homo sapiens
103 ZNRF2 223082
Affinity Capture-MS Homo sapiens
104 KRT8 3856
Proximity Label-MS Homo sapiens
105 C16orf72 29035
Affinity Capture-MS Homo sapiens
106 C2CD5 9847
Proximity Label-MS Homo sapiens
107 DNAJC1 64215
Affinity Capture-MS Homo sapiens
108 MOB1A 55233
Proximity Label-MS Homo sapiens
109 PLD6  
Affinity Capture-MS Homo sapiens
110 RPA3 6119
Proximity Label-MS Homo sapiens
111 NOTCH1 4851
Affinity Capture-MS Homo sapiens
112 C17orf59 54785
Proximity Label-MS Homo sapiens
113 S1PR4  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here