Gene description for GABARAPL2
Gene name GABA(A) receptor-associated protein-like 2
Gene symbol GABARAPL2
Other names/aliases ATG8
ATG8C
GATE-16
GATE16
GEF-2
GEF2
Species Homo sapiens
 Database cross references - GABARAPL2
ExoCarta ExoCarta_11345
Vesiclepedia VP_11345
Entrez Gene 11345
HGNC 13291
MIM 607452
UniProt P60520  
 GABARAPL2 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for GABARAPL2
Molecular Function
    SNARE binding GO:0000149 ISS
    protein binding GO:0005515 IPI
    microtubule binding GO:0008017 NAS
    phosphatidylethanolamine binding GO:0008429 IBA
    phosphatidylethanolamine binding GO:0008429 IDA
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
    beta-tubulin binding GO:0048487 ISS
    GABA receptor binding GO:0050811 ISS
    GABA receptor binding GO:0050811 NAS
    ATPase binding GO:0051117 ISS
Biological Process
    autophagosome assembly GO:0000045 IBA
    mitophagy GO:0000423 IBA
    intra-Golgi vesicle-mediated transport GO:0006891 ISS
    autophagy GO:0006914 NAS
    cellular response to nitrogen starvation GO:0006995 IBA
    protein transport GO:0015031 IEA
    positive regulation of ATP-dependent activity GO:0032781 ISS
    protein localization to endoplasmic reticulum GO:0070972 IDA
    autophagosome maturation GO:0097352 IBA
    negative regulation of proteasomal protein catabolic process GO:1901799 IMP
Subcellular Localization
    Golgi membrane GO:0000139 IDA
    Golgi membrane GO:0000139 ISS
    autophagosome membrane GO:0000421 IBA
    autophagosome membrane GO:0000421 IDA
    autophagosome membrane GO:0000421 TAS
    cytoplasm GO:0005737 IDA
    autophagosome GO:0005776 IDA
    endoplasmic reticulum membrane GO:0005789 IEA
    Golgi apparatus GO:0005794 ISS
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    cytoplasmic vesicle GO:0031410 IEA
 Experiment description of studies that identified GABARAPL2 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GABARAPL2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GABARAPL1 23710
Affinity Capture-MS Homo sapiens
2 GLRX5 51218
Co-fractionation Homo sapiens
3 MAP1LC3C  
Affinity Capture-MS Homo sapiens
4 TNIP1 10318
Two-hybrid Homo sapiens
5 VAPA 9218
Affinity Capture-Western Homo sapiens
6 HACD3 51495
Affinity Capture-MS Homo sapiens
7 KIAA1958  
Two-hybrid Homo sapiens
8 CALCOCO1 57658
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 BNIP3L  
Affinity Capture-Western Homo sapiens
10 TXNDC12 51060
Co-fractionation Homo sapiens
11 FFAR1  
Affinity Capture-MS Homo sapiens
12 APP 351
Reconstituted Complex Homo sapiens
13 MEFV  
Reconstituted Complex Homo sapiens
14 LRPPRC 10128
Affinity Capture-MS Homo sapiens
15 MAP1LC3B 81631
Reconstituted Complex Homo sapiens
16 WDR62  
Affinity Capture-MS Homo sapiens
17 AK3 50808
Co-fractionation Homo sapiens
18 AHNAK2 113146
Two-hybrid Homo sapiens
19 CALCOCO2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
20 HADHB 3032
Affinity Capture-MS Homo sapiens
21 FXN  
Co-fractionation Homo sapiens
22 TP53INP2  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
23 ATG7 10533
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
24 ATG4D  
Biochemical Activity Homo sapiens
25 WDFY3 23001
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
26 VDR  
Two-hybrid Homo sapiens
27 CLN8 2055
Two-hybrid Homo sapiens
28 GUK1 2987
Co-fractionation Homo sapiens
29 NEDD4 4734
Affinity Capture-MS Homo sapiens
30 RPS2 6187
Two-hybrid Homo sapiens
31 CCT2 10576
Affinity Capture-Western Homo sapiens
32 TP53INP1  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
33 SMO 6608
Two-hybrid Homo sapiens
34 ATG4A  
Biochemical Activity Homo sapiens
35 PRDX5 25824
Co-fractionation Homo sapiens
36 GIMAP6  
Two-hybrid Homo sapiens
37 NBR1 4077
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
38 OPTN 10133
Reconstituted Complex Homo sapiens
39 FUNDC1  
Two-hybrid Homo sapiens
40 AGTRAP 57085
Two-hybrid Homo sapiens
41 ULK2  
Protein-peptide Homo sapiens
42 NUDC 10726
Cross-Linking-MS (XL-MS) Homo sapiens
43 GABARAP 11337
Affinity Capture-MS Homo sapiens
44 TRIM21 6737
Reconstituted Complex Homo sapiens
45 MLX  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
46 RAP1A 5906
Biochemical Activity Homo sapiens
47 TBC1D5 9779
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
48 ULK1  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
49 RAB11FIP5 26056
Co-fractionation Homo sapiens
50 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
51 TSR2  
Two-hybrid Homo sapiens
52 IRGQ 126298
Affinity Capture-MS Homo sapiens
53 RAP2A 5911
Biochemical Activity Homo sapiens
54 KRTAP10-7  
Two-hybrid Homo sapiens
55 TXN2 25828
Co-fractionation Homo sapiens
56 IPO5 3843
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 AK2 204
Co-fractionation Homo sapiens
58 HADHA 3030
Affinity Capture-MS Homo sapiens
59 GIMAP8 155038
Co-fractionation Homo sapiens
60 TAX1BP1 8887
Two-hybrid Homo sapiens
61 AMBRA1  
Protein-peptide Homo sapiens
62 TMEM160 54958
Affinity Capture-MS Homo sapiens
63 EIF4G1 1981
Affinity Capture-MS Homo sapiens
64 CCT6A 908
Affinity Capture-Western Homo sapiens
65 PTPRA 5786
Two-hybrid Homo sapiens
66 CUL3 8452
Affinity Capture-MS Homo sapiens
67 YWHAZ 7534
Two-hybrid Homo sapiens
68 EGFR 1956
PCA Homo sapiens
69 CCDC155  
Two-hybrid Homo sapiens
70 KBTBD7  
Two-hybrid Homo sapiens
71 NPSR1  
Two-hybrid Homo sapiens
72 PRKCI 5584
Affinity Capture-MS Homo sapiens
73 KLHL5  
Two-hybrid Homo sapiens
74 PSAP 5660
Co-fractionation Homo sapiens
75 MSRB3 253827
Co-fractionation Homo sapiens
76 RCN2 5955
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
77 STBD1 8987
Affinity Capture-MS Homo sapiens
78 SGTA 6449
Two-hybrid Homo sapiens
79 VDAC1 7416
Affinity Capture-MS Homo sapiens
80 SIK2  
Two-hybrid Homo sapiens
81 TBC1D9B 23061
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
82 TBC1D25  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
83 GBAS 2631
Affinity Capture-MS Homo sapiens
84 AK4 205
Co-fractionation Homo sapiens
85 TP53 7157
Affinity Capture-MS Homo sapiens
86 UBA5 79876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
87 WWOX 51741
Affinity Capture-MS Homo sapiens
88 TBC1D9  
Two-hybrid Homo sapiens
89 ISCA2 122961
Co-fractionation Homo sapiens
90 ATG13 9776
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
91 EZR 7430
Affinity Capture-MS Homo sapiens
92 KEAP1 9817
Affinity Capture-MS Homo sapiens
93 TTBK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
94 SQSTM1 8878
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
95 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
96 NUDT19 390916
Co-fractionation Homo sapiens
97 TXN 7295
Co-fractionation Homo sapiens
98 CEBPA  
Protein-peptide Homo sapiens
99 BCL2L13 23786
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
100 ATG4B 23192
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
101 HBG1 3047
Affinity Capture-MS Homo sapiens
102 ARFGAP1 55738
Two-hybrid Homo sapiens
103 NEK9 91754
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 AAMP 14
Two-hybrid Homo sapiens
105 ATG3 64422
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 KRTAP10-9  
Two-hybrid Homo sapiens
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