Gene description for Smn1
Gene name survival motor neuron 1
Gene symbol Smn1
Other names/aliases AI849087
Gemin1
Smn
Species Mus musculus
 Database cross references - Smn1
ExoCarta ExoCarta_20595
Vesiclepedia VP_20595
Entrez Gene 20595
UniProt P97801  
 Smn1 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Smn1
Molecular Function
    RNA binding GO:0003723 IEA
    protein binding GO:0005515 IPI
    fibroblast growth factor binding GO:0017134 ISO
    identical protein binding GO:0042802 ISO
Biological Process
    spliceosomal snRNP assembly GO:0000387 IBA
    spliceosomal snRNP assembly GO:0000387 ISS
    DNA-templated transcription termination GO:0006353 ISS
    microtubule depolymerization GO:0007019 IMP
    axonogenesis GO:0007409 IMP
    regulation of neuron projection development GO:0010975 IGI
    positive regulation of RNA splicing GO:0033120 IMP
Subcellular Localization
    nucleus GO:0005634 IBA
    nucleus GO:0005634 IDA
    nucleus GO:0005634 ISO
    nucleoplasm GO:0005654 ISO
    nucleoplasm GO:0005654 ISS
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 ISO
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    Cajal body GO:0015030 IBA
    Cajal body GO:0015030 IDA
    Cajal body GO:0015030 ISO
    Cajal body GO:0015030 ISS
    nuclear body GO:0016604 ISO
    Z disc GO:0030018 IEA
    COPI-coated vesicle GO:0030137 IDA
    growth cone GO:0030426 IDA
    SMN complex GO:0032797 IBA
    SMN complex GO:0032797 ISO
    SMN complex GO:0032797 ISS
    SMN-Sm protein complex GO:0034719 ISO
    SMN-Sm protein complex GO:0034719 ISS
    cytoplasmic ribonucleoprotein granule GO:0036464 ISO
    cytoplasmic ribonucleoprotein granule GO:0036464 ISS
    neuron projection GO:0043005 ISO
    neuron projection GO:0043005 ISS
    perikaryon GO:0043204 ISO
    perikaryon GO:0043204 ISS
    Gemini of coiled bodies GO:0097504 IBA
    Gemini of coiled bodies GO:0097504 ISO
    Gemini of coiled bodies GO:0097504 ISS
 Experiment description of studies that identified Smn1 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Smn1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Affinity Capture-MS Homo sapiens
2 GEMIN6  
Affinity Capture-MS Homo sapiens
3 PRSS56  
Affinity Capture-MS Homo sapiens
4 AFG3L2 10939
Affinity Capture-MS Homo sapiens
5 DOCK1 1793
Affinity Capture-MS Homo sapiens
6 GLUD1 2746
Affinity Capture-MS Homo sapiens
7 ALDH3A2 224
Affinity Capture-MS Homo sapiens
8 Imp1  
Affinity Capture-Western Mus musculus
Affinity Capture-Western Mus musculus
9 SLC35E1 79939
Affinity Capture-MS Homo sapiens
10 SMN1 6606
Two-hybrid Homo sapiens
11 HADHB 3032
Affinity Capture-MS Homo sapiens
12 COIL  
Affinity Capture-MS Homo sapiens
13 GEMIN2 8487
Affinity Capture-MS Homo sapiens
14 ATP5I 521
Affinity Capture-MS Homo sapiens
15 DHCR7 1717
Affinity Capture-MS Homo sapiens
16 BAG2 9532
Affinity Capture-MS Homo sapiens
17 NOP9 161424
Affinity Capture-MS Homo sapiens
18 VKORC1 79001
Affinity Capture-MS Homo sapiens
19 SNRPB 6628
Affinity Capture-MS Homo sapiens
20 KRT8 3856
Affinity Capture-MS Homo sapiens
21 Cdk2  
Co-localization Mus musculus
22 Ikbkg  
Affinity Capture-Western Mus musculus
23 IQGAP3 128239
Affinity Capture-MS Homo sapiens
24 ELAVL4  
FRET Homo sapiens
25 ATP5A1 498
Affinity Capture-MS Homo sapiens
26 SLC1A5 6510
Affinity Capture-MS Homo sapiens
27 TMEM161A  
Affinity Capture-MS Homo sapiens
28 Coil  
Co-localization Mus musculus
29 GEMIN8 54960
Affinity Capture-MS Homo sapiens
30 INTS3 65123
Affinity Capture-MS Homo sapiens
31 PINX1  
Affinity Capture-MS Homo sapiens
32 SLC7A5 8140
Affinity Capture-MS Homo sapiens
33 PTDSS1 9791
Affinity Capture-MS Homo sapiens
34 PHAX  
Affinity Capture-MS Homo sapiens
35 TRAM1 23471
Affinity Capture-MS Homo sapiens
36 GEMIN4 50628
Affinity Capture-MS Homo sapiens
37 CLNS1A 1207
Affinity Capture-MS Homo sapiens
38 SNRPD2 6633
Affinity Capture-MS Homo sapiens
39 GDF15 9518
Affinity Capture-MS Homo sapiens
40 LSM10  
Affinity Capture-MS Homo sapiens
41 TIMM50 92609
Affinity Capture-MS Homo sapiens
42 STRAP 11171
Affinity Capture-MS Homo sapiens
43 KLHL8  
Affinity Capture-MS Homo sapiens
44 Igf2bp1  
Affinity Capture-Western Rattus norvegicus
Affinity Capture-Western Rattus norvegicus
FRET Rattus norvegicus
45 TXN 7295
Affinity Capture-MS Homo sapiens
46 SLC38A5 92745
Affinity Capture-MS Homo sapiens
47 Traf6  
Affinity Capture-Western Mus musculus
48 SNRNP70 6625
Affinity Capture-MS Homo sapiens
49 DDX20 11218
Affinity Capture-MS Homo sapiens
50 PPP2R3C  
Affinity Capture-MS Homo sapiens
51 SNRPD1 6632
Affinity Capture-MS Homo sapiens
52 RNF5  
Affinity Capture-MS Homo sapiens
53 CAMSAP3  
Affinity Capture-MS Homo sapiens
54 COX11  
Affinity Capture-MS Homo sapiens
55 TXLNG  
Affinity Capture-MS Homo sapiens
56 PNKD  
Affinity Capture-MS Homo sapiens
57 C19orf70  
Affinity Capture-MS Homo sapiens
58 LSM11  
Affinity Capture-MS Homo sapiens
59 GEMIN5 25929
Affinity Capture-MS Homo sapiens
60 ELOVL1 64834
Affinity Capture-MS Homo sapiens
61 EGFL7 51162
Affinity Capture-MS Homo sapiens
62 RAF1 5894
Affinity Capture-MS Homo sapiens
63 TIMM23 100287932
Affinity Capture-MS Homo sapiens
64 CLPTM1L 81037
Affinity Capture-MS Homo sapiens
65 TIA1 7072
Affinity Capture-MS Homo sapiens
66 Chuk  
Affinity Capture-Western Mus musculus
67 FBL 2091
Affinity Capture-MS Homo sapiens
68 GEMIN7  
Affinity Capture-MS Homo sapiens
69 NCBP2-AS2  
Affinity Capture-MS Homo sapiens
70 TMEM209 84928
Affinity Capture-MS Homo sapiens
71 ESYT2 57488
Affinity Capture-MS Homo sapiens
72 PHLDB2 90102
Affinity Capture-MS Homo sapiens
73 Ikbkb 16150
Affinity Capture-Western Mus musculus
74 GGCX 2677
Affinity Capture-MS Homo sapiens
75 Tcerg1  
Affinity Capture-Western Mus musculus
Far Western Mus musculus
Affinity Capture-Western Mus musculus
76 Smn1 20595
Two-hybrid Mus musculus
Two-hybrid Mus musculus
77 PRKACB 5567
Affinity Capture-MS Homo sapiens
78 Snrpb 20638
Affinity Capture-Western Mus musculus
79 DERL2 51009
Affinity Capture-MS Homo sapiens
80 CDKN2A 1029
Affinity Capture-MS Homo sapiens
81 SNRPD3 6634
Affinity Capture-MS Homo sapiens
82 COX4I1 1327
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which Smn1 is involved
PathwayEvidenceSource
Metabolism of non-coding RNA IEA Reactome
Metabolism of RNA IEA Reactome
snRNP Assembly IEA Reactome





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