Gene description for MAP1LC3A
Gene name microtubule-associated protein 1 light chain 3 alpha
Gene symbol MAP1LC3A
Other names/aliases ATG8E
LC3
LC3A
MAP1ALC3
MAP1BLC3
Species Homo sapiens
 Database cross references - MAP1LC3A
ExoCarta ExoCarta_84557
Vesiclepedia VP_84557
Entrez Gene 84557
HGNC 6838
MIM 601242
UniProt Q9H492  
 MAP1LC3A identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for MAP1LC3A
Molecular Function
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IDA
    microtubule binding GO:0008017 IBA
    phosphatidylethanolamine binding GO:0008429 IBA
    ubiquitin protein ligase binding GO:0031625 IBA
    ubiquitin protein ligase binding GO:0031625 IPI
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 IMP
    autophagosome assembly GO:0000045 ISS
    autophagy of mitochondrion GO:0000422 IGI
    mitophagy GO:0000423 IBA
    cellular response to nitrogen starvation GO:0006995 IBA
    JNK cascade GO:0007254 IEA
    cellular response to starvation GO:0009267 IDA
    response to iron(II) ion GO:0010040 IEA
    response to lead ion GO:0010288 IEA
    macroautophagy GO:0016236 IDA
    cellular response to amino acid starvation GO:0034198 IEA
    p38MAPK cascade GO:0038066 IEA
    SMAD protein signal transduction GO:0060395 IEA
    cellular response to hydrogen peroxide GO:0070301 IEA
    cellular response to copper ion GO:0071280 IEA
    cellular response to oxygen-glucose deprivation GO:0090650 IEA
    autophagosome maturation GO:0097352 IBA
    autophagosome maturation GO:0097352 IDA
Subcellular Localization
    autophagosome membrane GO:0000421 IBA
    autophagosome membrane GO:0000421 TAS
    late endosome GO:0005770 IEA
    autophagosome GO:0005776 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    organelle membrane GO:0031090 IDA
    intracellular membrane-bounded organelle GO:0043231 IDA
    autolysosome GO:0044754 IDA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified MAP1LC3A in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAP1LC3A
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-MS Homo sapiens
2 GABARAPL1 23710
Co-localization Homo sapiens
3 RAB7B  
Affinity Capture-Western Homo sapiens
4 PRKAA1 5562
Affinity Capture-Western Homo sapiens
5 TNIP1 10318
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
6 TBC1D10A 83874
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 CCT3 7203
Affinity Capture-Western Homo sapiens
8 SHC1 6464
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 TBC1D2 55357
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
10 AKAP13 11214
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
11 MTX1 4580
Reconstituted Complex Homo sapiens
12 MOAP1  
Affinity Capture-Western Homo sapiens
13 VPRBP 9730
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
14 DDX17 10521
Affinity Capture-MS Homo sapiens
15 TBC1D16  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 CALCOCO1 57658
Affinity Capture-Western Homo sapiens
17 UBC 7316
Co-purification Homo sapiens
Co-localization Homo sapiens
18 BNIP3L  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
19 PRPF40A 55660
Two-hybrid Homo sapiens
20 APP 351
Reconstituted Complex Homo sapiens
21 MEFV  
Reconstituted Complex Homo sapiens
22 ATG13 9776
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
23 MAP1LC3B 81631
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 HSPA2 3306
Affinity Capture-MS Homo sapiens
25 MAP1B 4131
Affinity Capture-MS Homo sapiens
26 OPRM1 4988
Two-hybrid Homo sapiens
27 CALCOCO2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
28 UBQLN1 29979
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
29 HADHA 3030
Affinity Capture-MS Homo sapiens
30 RASSF5 83593
Affinity Capture-MS Homo sapiens
31 TP53INP2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 ATG7 10533
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
33 WDFY3 23001
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
34 MAVS 57506
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
35 UBQLN4 56893
Affinity Capture-Western Homo sapiens
36 DVL2 1856
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
37 DCP1B  
Affinity Capture-MS Homo sapiens
38 TBC1D1 23216
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
39 CCT2 10576
Co-fractionation Homo sapiens
Affinity Capture-Western Homo sapiens
40 TBC1D17 79735
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
41 TP53INP1  
Affinity Capture-Western Homo sapiens
FRET Homo sapiens
42 TOLLIP 54472
Affinity Capture-Western Homo sapiens
43 GPSM1 26086
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
44 TCP1 6950
Affinity Capture-Western Homo sapiens
45 SAMM50 25813
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
46 PSMD4 5710
Affinity Capture-Western Homo sapiens
47 RB1CC1 9821
Affinity Capture-Western Homo sapiens
48 RPL4 6124
Affinity Capture-MS Homo sapiens
49 CLU 1191
Affinity Capture-Western Homo sapiens
50 PIAS4  
Two-hybrid Homo sapiens
51 NBR1 4077
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
52 OPTN 10133
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
53 FUNDC1  
Two-hybrid Homo sapiens
54 CLTCL1 8218
Affinity Capture-MS Homo sapiens
55 HTT 3064
Affinity Capture-Western Homo sapiens
56 EPAS1  
Affinity Capture-Western Homo sapiens
57 TUBA1B 10376
Affinity Capture-MS Homo sapiens
58 SAFB2 9667
Affinity Capture-MS Homo sapiens
59 VCP 7415
Co-localization Homo sapiens
60 DDX5 1655
Affinity Capture-MS Homo sapiens
61 CBX5 23468
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
62 TBC1D5 9779
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
63 ULK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
64 Sqstm1  
Affinity Capture-Western Mus musculus
65 RAB3GAP1 22930
Affinity Capture-MS Homo sapiens
66 EPG5 57724
Affinity Capture-Western Homo sapiens
67 PIK3C2A 5286
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 PELP1 27043
Affinity Capture-MS Homo sapiens
69 PGAP1 80055
Affinity Capture-MS Homo sapiens
70 NIPSNAP1 8508
Affinity Capture-MS Homo sapiens
71 SRPK1 6732
Biochemical Activity Homo sapiens
72 IRGQ 126298
Two-hybrid Homo sapiens
73 ASAP2 8853
Affinity Capture-MS Homo sapiens
74 TRIM21 6737
Reconstituted Complex Homo sapiens
75 PHB 5245
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
76 STK3 6788
Affinity Capture-MS Homo sapiens
77 HSPA5 3309
Affinity Capture-MS Homo sapiens
78 TUBB 203068
Affinity Capture-MS Homo sapiens
79 IPO5 3843
Affinity Capture-MS Homo sapiens
80 HADHB 3032
Affinity Capture-MS Homo sapiens
81 CLINT1 9685
Affinity Capture-MS Homo sapiens
82 KIF15 56992
Two-hybrid Homo sapiens
83 AMBRA1  
Affinity Capture-Western Homo sapiens
Protein-peptide Homo sapiens
84 HPCAL1 3241
Affinity Capture-Western Homo sapiens
85 CCT6A 908
Affinity Capture-Western Homo sapiens
86 CHDH 55349
Affinity Capture-Western Homo sapiens
87 TBC1D10B 26000
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
88 SYNPO2  
Affinity Capture-Western Homo sapiens
89 TMEM160 54958
Affinity Capture-MS Homo sapiens
90 TUBB4B 10383
Affinity Capture-MS Homo sapiens
91 EGFR 1956
Negative Genetic Homo sapiens
Affinity Capture-MS Homo sapiens
92 ATG14  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
93 PDPK1 5170
Affinity Capture-Western Homo sapiens
94 EPHA7 2045
Two-hybrid Homo sapiens
95 PRKCI 5584
Affinity Capture-MS Homo sapiens
96 PDCD4 27250
Affinity Capture-Western Homo sapiens
97 FAM134C 162427
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
98 MYC  
Affinity Capture-MS Homo sapiens
99 HSPA1A 3303
Affinity Capture-MS Homo sapiens
100 RABGAP1L 9910
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
101 RABGAP1 23637
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
102 MATR3 9782
Affinity Capture-MS Homo sapiens
103 BNIP3 664
Affinity Capture-Western Homo sapiens
104 CLTC 1213
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
105 STK4 6789
Affinity Capture-MS Homo sapiens
106 TBC1D9B 23061
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
107 NSMAF  
Affinity Capture-MS Homo sapiens
108 MDM2  
Biochemical Activity Homo sapiens
109 TBC1D25  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
110 GBAS 2631
Affinity Capture-MS Homo sapiens
111 SBDS 51119
Affinity Capture-MS Homo sapiens
112 SNCA 6622
Co-localization Homo sapiens
113 PHB2 11331
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
114 RRBP1 6238
Affinity Capture-MS Homo sapiens
115 RAB3GAP2 25782
Affinity Capture-MS Homo sapiens
116 MAP1S 55201
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
117 TBC1D7  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
118 PDCD6IP 10015
Affinity Capture-MS Homo sapiens
119 TBC1D9  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
120 RTKN 6242
Reconstituted Complex Homo sapiens
121 LGALS3 3958
Co-localization Homo sapiens
122 KEAP1 9817
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
123 SAFB 6294
Affinity Capture-MS Homo sapiens
124 CASP8 841
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
125 HNRNPM 4670
Affinity Capture-MS Homo sapiens
126 SQSTM1 8878
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Protein-peptide Homo sapiens
127 ATG101  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
128 PRKAG2 51422
Affinity Capture-MS Homo sapiens
129 FYCO1 79443
Affinity Capture-MS Homo sapiens
Protein-peptide Homo sapiens
Affinity Capture-MS Homo sapiens
130 BCL10  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
131 TBC1D2B 23102
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
132 ATG4B 23192
Co-crystal Structure Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
133 PINK1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
134 CLTA 1211
Affinity Capture-MS Homo sapiens
135 KXD1 79036
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
136 PADI3 51702
Affinity Capture-MS Homo sapiens
137 RAB33B 83452
Affinity Capture-Western Homo sapiens
138 SERBP1 26135
Affinity Capture-MS Homo sapiens
139 RDH12 145226
Two-hybrid Homo sapiens
140 HNRNPU 3192
Affinity Capture-MS Homo sapiens
141 MAP1A 4130
Affinity Capture-MS Homo sapiens
142 NOTCH1 4851
Affinity Capture-Western Homo sapiens
143 EPRS 2058
Affinity Capture-MS Homo sapiens
144 ATG3 64422
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
View the network image/svg+xml



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