Gene description for DNAH8
Gene name dynein, axonemal, heavy chain 8
Gene symbol DNAH8
Other names/aliases ATPase
hdhc9
Species Homo sapiens
 Database cross references - DNAH8
ExoCarta ExoCarta_1769
Vesiclepedia VP_1769
Entrez Gene 1769
HGNC 2952
MIM 603337
UniProt Q96JB1  
 DNAH8 identified in sEVs derived from the following tissue/cell type
Urine 15326289    
Urine 19056867    
 Gene ontology annotations for DNAH8
Molecular Function
    microtubule motor activity GO:0003777 IMP
    microtubule motor activity GO:0003777 NAS
    ATP binding GO:0005524 IEA
    minus-end-directed microtubule motor activity GO:0008569 IBA
    ATP hydrolysis activity GO:0016887 IEA
    dynein intermediate chain binding GO:0045505 IBA
    dynein light intermediate chain binding GO:0051959 IBA
Biological Process
    outer dynein arm assembly GO:0036158 IBA
    cilium-dependent cell motility GO:0060285 NAS
    cilium movement involved in cell motility GO:0060294 IBA
Subcellular Localization
    axonemal dynein complex GO:0005858 NAS
    microtubule GO:0005874 IEA
    axoneme GO:0005930 IDA
    axoneme GO:0005930 IMP
    sperm flagellum GO:0036126 IBA
    sperm flagellum GO:0036126 IDA
    sperm flagellum GO:0036126 IMP
    outer dynein arm GO:0036157 IBA
 Experiment description of studies that identified DNAH8 in sEVs
1
Experiment ID 13
MISEV standards
✔
IEM
Biophysical techniques
✔
Alix|RAB4|RAB5B|RAB11|TSG101|CD9|AQP2|AQP1
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors "Pisitkun T, Shen RF, Knepper MA"
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
2
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for DNAH8
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HSPA4 3308
Cross-Linking-MS (XL-MS) Homo sapiens
2 TOMM40 10452
Co-fractionation Homo sapiens
3 TOMM22 56993
Co-fractionation Homo sapiens
4 EIF5A 1984
Cross-Linking-MS (XL-MS) Homo sapiens
5 ENO1 2023
Cross-Linking-MS (XL-MS) Homo sapiens
6 GAN 8139
Affinity Capture-MS Homo sapiens
7 VDAC3 7419
Co-fractionation Homo sapiens
8 HADHB 3032
Co-fractionation Homo sapiens
9 EGFR 1956
Affinity Capture-MS Homo sapiens
10 RPL19 6143
Cross-Linking-MS (XL-MS) Homo sapiens
Cross-Linking-MS (XL-MS) Homo sapiens
11 VDAC2 7417
Co-fractionation Homo sapiens
12 HIST1H3A 8350
Cross-Linking-MS (XL-MS) Homo sapiens
13 HNRNPA2B1 3181
Cross-Linking-MS (XL-MS) Homo sapiens
14 KMT2A  
Cross-Linking-MS (XL-MS) Homo sapiens
View the network image/svg+xml
 Pathways in which DNAH8 is involved
No pathways found





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