Gene description for NFIX
Gene name nuclear factor I/X (CCAAT-binding transcription factor)
Gene symbol NFIX
Other names/aliases MRSHSS
Species Homo sapiens
 Database cross references - NFIX
ExoCarta ExoCarta_4784
Entrez Gene 4784
HGNC 7788
MIM 164005
UniProt Q14938  
 NFIX identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for NFIX
Molecular Function
    RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity GO:0003705 TAS
    DNA binding GO:0003677 IEA
    protein binding GO:0005515 IPI
    sequence-specific DNA binding transcription factor activity GO:0003700 TAS
Biological Process
    cerebellar granule cell differentiation GO:0021707 IEA
    cerebellar Purkinje cell layer development GO:0021680 IEA
    negative regulation of transcription from RNA polymerase II promoter GO:0000122 IDA
    transcription from RNA polymerase II promoter GO:0006366 TAS
    astrocyte differentiation GO:0048708 IEA
    DNA replication GO:0006260 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
Subcellular Localization
    nucleus GO:0005634 IBA
 Experiment description of studies that identified NFIX in exosomes
Experiment ID 236
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for NFIX
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RB1 5925
Affinity Capture-Western Homo sapiens
2 RFX1  
Affinity Capture-Western Homo sapiens
3 HDAC1 3065
Affinity Capture-Western Homo sapiens
4 NFIB  
Invitro Homo sapiens
5 PIR 8544
Invivo Homo sapiens
Two-hybrid Homo sapiens
6 SKI 6497
Reconstituted Complex Homo sapiens
Phenotypic Enhancement Homo sapiens
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