Gene description for SAP30
Gene name Sin3A-associated protein, 30kDa
Gene symbol SAP30
Other names/aliases -
Species Homo sapiens
 Database cross references - SAP30
ExoCarta ExoCarta_8819
Vesiclepedia VP_8819
Entrez Gene 8819
HGNC 10532
MIM 603378
UniProt O75446  
 SAP30 identified in exosomes derived from the following tissue/cell type
Neuroblastoma cells 25944692    
 Gene ontology annotations for SAP30
Molecular Function
    DNA binding GO:0003677 IEA
    transcription coregulator activity GO:0003712 IBA
    transcription corepressor activity GO:0003714 IDA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IDA
    negative regulation of transcription by RNA polymerase II GO:0000122 NAS
    regulation of DNA-templated transcription GO:0006355 IBA
    negative regulation of cell migration GO:0030336 NAS
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 NAS
    skeletal muscle cell differentiation GO:0035914 IEA
    modulation by host of symbiont transcription GO:0052472 IDA
    negative regulation of stem cell population maintenance GO:1902455 NAS
    positive regulation of stem cell population maintenance GO:1902459 NAS
Subcellular Localization
    histone deacetylase complex GO:0000118 IBA
    histone deacetylase complex GO:0000118 IDA
    nucleus GO:0005634 NAS
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    Sin3-type complex GO:0070822 NAS
 Experiment description of studies that identified SAP30 in exosomes
1
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
 Protein-protein interactions for SAP30
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HIST2H2BE 8349
Reconstituted Complex Homo sapiens
2 C15orf40  
Affinity Capture-MS Homo sapiens
3 GATAD2B 57459
Affinity Capture-MS Homo sapiens
4 HDAC2 3066
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
5 ZNF296  
Affinity Capture-MS Homo sapiens
6 DNTTIP1 116092
Co-fractionation Homo sapiens
7 SUZ12  
Affinity Capture-MS Homo sapiens
8 TGIF2LX  
Affinity Capture-MS Homo sapiens
9 TNRC18  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 RPL15 6138
Affinity Capture-MS Homo sapiens
11 Cul3  
Affinity Capture-MS Mus musculus
12 CSNK2A1 1457
Affinity Capture-MS Homo sapiens
13 VHL  
Two-hybrid Homo sapiens
14 DECR1 1666
Affinity Capture-MS Homo sapiens
15 CMBL 134147
Affinity Capture-MS Homo sapiens
16 CPSF2 53981
Co-fractionation Homo sapiens
17 CHD4 1108
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
18 HDAC3 8841
Affinity Capture-Western Homo sapiens
19 NCOR1  
Co-purification Homo sapiens
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
20 MTF2  
Affinity Capture-MS Homo sapiens
21 BCL7B  
Affinity Capture-MS Homo sapiens
22 CHAF1A  
Affinity Capture-MS Homo sapiens
23 MXD3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 SAP25  
Affinity Capture-MS Homo sapiens
25 SMARCA1 6594
Affinity Capture-MS Homo sapiens
26 CDK2AP1  
Affinity Capture-MS Homo sapiens
27 PCBD1 5092
Two-hybrid Homo sapiens
28 ARID4B  
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
29 ZNF512B  
Affinity Capture-MS Homo sapiens
30 Fam60a 56306
Affinity Capture-MS Mus musculus
31 BRPF3  
Affinity Capture-MS Homo sapiens
32 EZH1  
Affinity Capture-MS Homo sapiens
33 EED  
Affinity Capture-MS Homo sapiens
34 ZNF219  
Affinity Capture-MS Homo sapiens
35 ASH2L 9070
Co-fractionation Homo sapiens
36 PHF1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
37 MYCN  
Affinity Capture-MS Homo sapiens
38 GPS1 2873
Co-fractionation Homo sapiens
39 CHD3 1107
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
40 SMARCB1 6598
Co-fractionation Homo sapiens
41 SMARCA2 6595
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
42 MTA2 9219
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
43 BCL11B  
Affinity Capture-MS Homo sapiens
44 GAR1 54433
Affinity Capture-MS Homo sapiens
45 HSPA8 3312
Co-fractionation Homo sapiens
46 ZNF217 7764
Co-fractionation Homo sapiens
47 H2AFZ 3015
Affinity Capture-MS Homo sapiens
48 RBBP7 5931
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
49 SYMPK 8189
Co-fractionation Homo sapiens
50 SHPRH  
Affinity Capture-MS Homo sapiens
51 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
52 SAP30L  
Affinity Capture-MS Homo sapiens
53 CUL4B 8450
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
54 SRA1 10011
Affinity Capture-MS Homo sapiens
55 BRMS1 25855
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 NCOR2  
Co-purification Homo sapiens
Co-fractionation Homo sapiens
57 C17orf49 124944
Affinity Capture-MS Homo sapiens
58 ZNF395 55893
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
59 MBD3 53615
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
60 LIN52  
Affinity Capture-MS Homo sapiens
61 UBASH3B 84959
Affinity Capture-MS Homo sapiens
62 ZCCHC17  
Affinity Capture-MS Homo sapiens
63 SMARCA4 6597
Co-fractionation Homo sapiens
64 SP1  
Co-fractionation Homo sapiens
65 RAN 5901
Co-fractionation Homo sapiens
66 OGT 8473
Co-fractionation Homo sapiens
67 FOXK2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
68 ACTL6A 86
Co-fractionation Homo sapiens
69 BPTF 2186
Affinity Capture-MS Homo sapiens
70 BRMS1L  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
71 MYBL2 4605
Affinity Capture-MS Homo sapiens
72 MBD2 8932
Affinity Capture-MS Homo sapiens
73 SMC1A 8243
Affinity Capture-MS Homo sapiens
74 CHD5 26038
Affinity Capture-MS Homo sapiens
75 SUDS3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
76 MRPL22  
Affinity Capture-MS Homo sapiens
77 LYST 1130
Affinity Capture-MS Homo sapiens
78 ID3  
Two-hybrid Homo sapiens
79 DKK2  
Affinity Capture-MS Homo sapiens
80 ARID4A  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
81 ING2  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
82 SMARCC2 6601
Co-fractionation Homo sapiens
83 BCL11A 53335
Affinity Capture-MS Homo sapiens
84 HSPA5 3309
Co-fractionation Homo sapiens
85 MIER1  
Co-fractionation Homo sapiens
86 ABT1 29777
Affinity Capture-MS Homo sapiens
87 SMARCD1 6602
Co-fractionation Homo sapiens
88 SMARCA5 8467
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
89 FAM60A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
90 HCFC1 3054
Co-fractionation Homo sapiens
91 ACTBL2 345651
Affinity Capture-MS Homo sapiens
92 MXD1  
Reconstituted Complex Homo sapiens
93 YY1 7528
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 RBBP4 5928
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
95 Max  
Affinity Capture-MS Mus musculus
96 EFTUD2 9343
Co-fractionation Homo sapiens
97 LIN9  
Affinity Capture-MS Homo sapiens
98 LDLR 3949
Positive Genetic Homo sapiens
99 CSNK2A2 1459
Affinity Capture-MS Homo sapiens
100 WDR5 11091
Co-fractionation Homo sapiens
101 HJURP  
Affinity Capture-MS Homo sapiens
102 HSPA4 3308
Co-fractionation Homo sapiens
103 BAHCC1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 DDB1 1642
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
105 ARID1A 8289
Co-fractionation Homo sapiens
106 RBP1 5947
Affinity Capture-Western Homo sapiens
107 HAT1 8520
Affinity Capture-MS Homo sapiens
108 RBBP5 5929
Co-fractionation Homo sapiens
109 FOXK1 221937
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 FHL2 2274
Affinity Capture-MS Homo sapiens
111 MDM2  
Affinity Capture-MS Homo sapiens
112 SIN3B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
113 GATAD2A 54815
Affinity Capture-MS Homo sapiens
114 TAF6 6878
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
115 CIR1  
Two-hybrid Homo sapiens
116 FNBP4  
Co-fractionation Homo sapiens
117 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
118 PARP1 142
Affinity Capture-MS Homo sapiens
119 ADAT3 113179
Affinity Capture-MS Homo sapiens
120 SAP130  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
121 MAX  
Affinity Capture-MS Homo sapiens
122 SIN3A  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
123 TGIF2  
Affinity Capture-MS Homo sapiens
124 PHF12  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
125 MTA1 9112
Affinity Capture-MS Homo sapiens
126 Sin3a  
Affinity Capture-MS Mus musculus
127 HDAC6 10013
Co-fractionation Homo sapiens
128 SMARCC1 6599
Co-fractionation Homo sapiens
129 SAP18 10284
Co-fractionation Homo sapiens
130 DPY30 84661
Affinity Capture-MS Homo sapiens
131 SAMSN1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
132 HDAC1 3065
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
133 EBP  
Affinity Capture-MS Homo sapiens
134 HSP90AA1 3320
Co-fractionation Homo sapiens
135 SIRT1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
136 ZNF704  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 HMGXB4 10042
Affinity Capture-MS Homo sapiens
138 MBNL1 4154
Affinity Capture-MS Homo sapiens
139 KDM1A 23028
Co-fractionation Homo sapiens
140 ING1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
141 EZH2  
Affinity Capture-MS Homo sapiens
142 PHF23 79142
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 MTA3 57504
Affinity Capture-MS Homo sapiens
144 GATAD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 CHAF1B  
Affinity Capture-MS Homo sapiens
146 PWWP2A  
Affinity Capture-MS Homo sapiens
147 CLIC2 1193
Affinity Capture-MS Homo sapiens
148 LIN37  
Affinity Capture-MS Homo sapiens
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