Gene description for TUBAL3
Gene name tubulin, alpha-like 3
Gene symbol TUBAL3
Other names/aliases -
Species Homo sapiens
 Database cross references - TUBAL3
ExoCarta ExoCarta_79861
Vesiclepedia VP_79861
Entrez Gene 79861
HGNC 23534
UniProt A6NHL2  
 TUBAL3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Neuroblastoma cells 25944692    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for TUBAL3
Molecular Function
    structural constituent of cytoskeleton GO:0005200 IBA
    GTP binding GO:0005525 IBA
    hydrolase activity GO:0016787 IEA
    metal ion binding GO:0046872 IEA
Biological Process
    microtubule cytoskeleton organization GO:0000226 IBA
    mitotic cell cycle GO:0000278 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    microtubule GO:0005874 IBA
 Experiment description of studies that identified TUBAL3 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 224
MISEV standards
EM|AFM
Biophysical techniques
Alix|TSG101|CD63|CD81
Enriched markers
GOLGA2
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors "Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
6
Experiment ID 191
MISEV standards
Biophysical techniques
Alix|CD81|CD9
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors "Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK."
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 217
MISEV standards
EM
Biophysical techniques
TSG101|CD81|CD9|CD63
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TUBAL3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2C 5432
Proximity Label-MS Homo sapiens
2 TFCP2 7024
Affinity Capture-MS Homo sapiens
3 AP2B1 163
Proximity Label-MS Homo sapiens
4 CCT3 7203
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 WRAP73 49856
Affinity Capture-MS Homo sapiens
6 SHC1 6464
Affinity Capture-MS Homo sapiens
7 FBXL6  
Affinity Capture-MS Homo sapiens
8 RIC8B 55188
Affinity Capture-MS Homo sapiens
9 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 NUP155 9631
Proximity Label-MS Homo sapiens
11 CCT6A 908
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 TCP1 6950
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
13 CCT4 10575
Affinity Capture-MS Homo sapiens
14 USP11 8237
Affinity Capture-MS Homo sapiens
15 CCT6B 10693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 PARK2  
Affinity Capture-MS Homo sapiens
17 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
18 DUSP16  
Affinity Capture-MS Homo sapiens
19 CCT7 10574
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 TXNDC9 10190
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CCT5 22948
Affinity Capture-MS Homo sapiens
22 KIAA1429 25962
Affinity Capture-MS Homo sapiens
23 TUBA4A 7277
Affinity Capture-MS Homo sapiens
24 PRKACA 5566
Affinity Capture-MS Homo sapiens
25 CAPS 828
Affinity Capture-MS Homo sapiens
26 YAP1 10413
Affinity Capture-MS Homo sapiens
27 DIRAS3  
Proximity Label-MS Homo sapiens
28 NEDD1 121441
Affinity Capture-MS Homo sapiens
29 TRIM31  
Affinity Capture-MS Homo sapiens
30 METTL3  
Affinity Capture-MS Homo sapiens
31 TCP11L2  
Affinity Capture-MS Homo sapiens
32 RNF2  
Affinity Capture-MS Homo sapiens
33 CYP2C9  
Proximity Label-MS Homo sapiens
34 AKAP1 8165
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TUBAL3 is involved
PathwayEvidenceSource
Activation of AMPK downstream of NMDARs IEA Reactome
Activation of NMDA receptors and postsynaptic events IEA Reactome
Adaptive Immune System TAS Reactome
Aggrephagy TAS Reactome
Aggrephagy IEA Reactome
Antiviral mechanism by IFN-stimulated genes TAS Reactome
Asparagine N-linked glycosylation TAS Reactome
Assembly and cell surface presentation of NMDA receptors IEA Reactome
Autophagy TAS Reactome
Autophagy IEA Reactome
Axon guidance TAS Reactome
Axon guidance IEA Reactome
Carboxyterminal post-translational modifications of tubulin IEA Reactome
Carboxyterminal post-translational modifications of tubulin TAS Reactome
Cell Cycle TAS Reactome
Cell Cycle, Mitotic TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Chaperonin-mediated protein folding IEA Reactome
Cilium Assembly TAS Reactome
Cooperation of Prefoldin and TriC/CCT in actin and tubulin folding IEA Reactome
COPI-dependent Golgi-to-ER retrograde traffic TAS Reactome
COPI-independent Golgi-to-ER retrograde traffic TAS Reactome
COPI-mediated anterograde transport TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Developmental Biology TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
EML4 and NUDC in mitotic spindle formation TAS Reactome
ER to Golgi Anterograde Transport TAS Reactome
Factors involved in megakaryocyte development and platelet production TAS Reactome
Formation of tubulin folding intermediates by CCT/TriC IEA Reactome
G2/M Transition TAS Reactome
Gap junction assembly IEA Reactome
Gap junction assembly TAS Reactome
Gap junction trafficking IEA Reactome
Gap junction trafficking TAS Reactome
Gap junction trafficking and regulation IEA Reactome
Gap junction trafficking and regulation TAS Reactome
Golgi-to-ER retrograde transport TAS Reactome
HCMV Early Events TAS Reactome
HCMV Infection TAS Reactome
Hemostasis TAS Reactome
HSP90 chaperone cycle for steroid hormone receptors (SHR) in the presence of ligand TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Interferon Signaling TAS Reactome
Intra-Golgi and retrograde Golgi-to-ER traffic TAS Reactome
Kinesins TAS Reactome
L1CAM interactions TAS Reactome
L1CAM interactions IEA Reactome
M Phase TAS Reactome
Macroautophagy TAS Reactome
Macroautophagy IEA Reactome
Membrane Trafficking IEA Reactome
Membrane Trafficking TAS Reactome
Metabolism of proteins IEA Reactome
Metabolism of proteins TAS Reactome
MHC class II antigen presentation TAS Reactome
Microtubule-dependent trafficking of connexons from Golgi to the plasma membrane TAS Reactome
Mitotic Anaphase TAS Reactome
Mitotic G2-G2/M phases TAS Reactome
Mitotic Metaphase and Anaphase TAS Reactome
Mitotic Prometaphase TAS Reactome
Nervous system development TAS Reactome
Nervous system development IEA Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
Nuclear Envelope (NE) Reassembly TAS Reactome
Organelle biogenesis and maintenance TAS Reactome
PKR-mediated signaling TAS Reactome
Post NMDA receptor activation events IEA Reactome
Post-chaperonin tubulin folding pathway IEA Reactome
Post-translational protein modification IEA Reactome
Post-translational protein modification TAS Reactome
Protein folding IEA Reactome
Recruitment of NuMA to mitotic centrosomes TAS Reactome
Recycling pathway of L1 TAS Reactome
Recycling pathway of L1 IEA Reactome
Resolution of Sister Chromatid Cohesion TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases Activate Formins TAS Reactome
RHO GTPases activate IQGAPs TAS Reactome
Sealing of the nuclear envelope (NE) by ESCRT-III TAS Reactome
Selective autophagy TAS Reactome
Selective autophagy IEA Reactome
Separation of Sister Chromatids TAS Reactome
Signal Transduction TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
The role of GTSE1 in G2/M progression after G2 checkpoint TAS Reactome
Translocation of SLC2A4 (GLUT4) to the plasma membrane IEA Reactome
Transmission across Chemical Synapses IEA Reactome
Transport of connexons to the plasma membrane TAS Reactome
Transport to the Golgi and subsequent modification TAS Reactome
Vesicle-mediated transport IEA Reactome
Vesicle-mediated transport TAS Reactome
Viral Infection Pathways TAS Reactome





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