Gene description for EIF3E
Gene name eukaryotic translation initiation factor 3, subunit E
Gene symbol EIF3E
Other names/aliases EIF3-P48
EIF3S6
INT6
eIF3-p46
Species Homo sapiens
 Database cross references - EIF3E
ExoCarta ExoCarta_3646
Entrez Gene 3646
HGNC 3277
MIM 602210
UniProt P60228  
 EIF3E identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Thymus 23844026    
 Gene ontology annotations for EIF3E
Molecular Function
    poly(A) RNA binding GO:0044822 IDA
    protein binding GO:0005515 IPI
    protein N-terminus binding GO:0047485 IPI
    translation initiation factor activity GO:0003743 IDA
Biological Process
    negative regulation of translational initiation GO:0045947 NAS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 IMP
    gene expression GO:0010467 TAS
    regulation of translational initiation GO:0006446 NAS
    translational initiation GO:0006413 TAS
    formation of translation preinitiation complex GO:0001731 IEA
    translation GO:0006412 TAS
    cellular protein metabolic process GO:0044267 TAS
Subcellular Localization
    nucleoplasm GO:0005654 NAS
    cytosol GO:0005829 TAS
    nucleus GO:0005634 IDA
    eukaryotic 48S preinitiation complex GO:0033290 IEA
    cytoplasm GO:0005737 IDA
    chromatin GO:0000785 NAS
    eukaryotic 43S preinitiation complex GO:0016282 IEA
    eukaryotic translation initiation factor 3 complex GO:0005852 NAS
    extracellular exosome GO:0070062 IDA
    membrane GO:0016020 IDA
    PML body GO:0016605 IDA
 Experiment description of studies that identified EIF3E in exosomes
1
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 81
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
4
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for EIF3E
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HAP1  
Two-hybrid Homo sapiens
2 EIF3A 8661
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
3 GPAA1  
Two-hybrid Homo sapiens
4 MAGED1  
Affinity Capture-MS Homo sapiens
5 RPIP8  
Two-hybrid Homo sapiens
6 SP192  
Two-hybrid Homo sapiens
7 SAP18 10284
Affinity Capture-MS Homo sapiens
8 COPS6 10980
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
9 IFIT1 3434
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
10 PRRC2A 7916
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
11 CNTF  
Affinity Capture-MS Homo sapiens
12 GC20  
Affinity Capture-MS Homo sapiens
13 SMAD9  
Affinity Capture-MS Homo sapiens
14 NSF 4905
Two-hybrid Homo sapiens
15 DDX24 57062
Two-hybrid Homo sapiens
16 COPS7B  
Affinity Capture-MS Homo sapiens
17 EIF3C 8663
Two-hybrid Homo sapiens
18 RGS2  
Affinity Capture-MS Homo sapiens
19 CSN3 1448
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
20 EIF3L 51386
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
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