Gene description for HAUS5
Gene name HAUS augmin-like complex, subunit 5
Gene symbol HAUS5
Other names/aliases KIAA0841
dgt5
Species Homo sapiens
 Database cross references - HAUS5
ExoCarta ExoCarta_23354
Vesiclepedia VP_23354
Entrez Gene 23354
HGNC 29130
MIM 613432
UniProt O94927  
 HAUS5 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for HAUS5
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    centrosome cycle GO:0007098 IBA
    centrosome cycle GO:0007098 IMP
    centrosome cycle GO:0007098 NAS
    regulation of microtubule nucleation GO:0010968 NAS
    spindle assembly GO:0051225 IBA
    spindle assembly GO:0051225 IMP
    spindle assembly GO:0051225 NAS
    cell division GO:0051301 IEA
Subcellular Localization
    centrosome GO:0005813 IBA
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    HAUS complex GO:0070652 IBA
    HAUS complex GO:0070652 IDA
    HAUS complex GO:0070652 IPI
    mitotic spindle microtubule GO:1990498 IDA
 Experiment description of studies that identified HAUS5 in exosomes
1
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for HAUS5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 RIT1 6016
Negative Genetic Homo sapiens
2 Lmna 16905
Affinity Capture-MS Mus musculus
3 HOOK1  
Proximity Label-MS Homo sapiens
4 CNKSR1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 USP43  
Affinity Capture-MS Homo sapiens
6 FAM167A  
Affinity Capture-MS Homo sapiens
7 CRYL1 51084
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TRIM66  
Affinity Capture-MS Homo sapiens
9 HDHD1  
Affinity Capture-MS Homo sapiens
10 HSPA1L 3305
Affinity Capture-MS Homo sapiens
11 KIF20A 10112
Affinity Capture-MS Homo sapiens
12 HIST3H2BB 128312
Affinity Capture-MS Homo sapiens
13 LOXL4 84171
Affinity Capture-MS Homo sapiens
14 SNX6 58533
Affinity Capture-MS Homo sapiens
15 TTC30B  
Affinity Capture-MS Homo sapiens
16 RNF10  
Affinity Capture-MS Homo sapiens
17 CDC16 8881
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 SYCE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 D2HGDH 728294
Affinity Capture-MS Homo sapiens
20 RFXANK  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 CHCHD2  
Affinity Capture-MS Homo sapiens
22 HAUS6  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
23 KRT78 196374
Affinity Capture-MS Homo sapiens
24 Ubr5  
Affinity Capture-MS Mus musculus
25 ATG7 10533
Affinity Capture-MS Homo sapiens
26 POLR2C 5432
Proximity Label-MS Homo sapiens
27 Haus1  
Affinity Capture-MS Mus musculus
28 IL17RA 23765
Affinity Capture-MS Homo sapiens
29 CIB2  
Affinity Capture-MS Homo sapiens
30 NPAS1  
Affinity Capture-MS Homo sapiens
31 C9orf163  
Affinity Capture-MS Homo sapiens
32 HAUS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 Haus4  
Affinity Capture-MS Mus musculus
34 PRPS2 5634
Affinity Capture-MS Homo sapiens
35 KRT8 3856
Proximity Label-MS Homo sapiens
36 HAUS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 S100A2 6273
Affinity Capture-MS Homo sapiens
38 SULT1C4  
Affinity Capture-MS Homo sapiens
39 MIB1 57534
Proximity Label-MS Homo sapiens
40 RGS2  
Two-hybrid Homo sapiens
41 PARK2  
Affinity Capture-MS Homo sapiens
42 B4GALT2  
Affinity Capture-MS Homo sapiens
43 EXOC4 60412
Affinity Capture-MS Homo sapiens
44 HAUS8  
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 TIGD5  
Affinity Capture-MS Homo sapiens
46 PPARD  
Affinity Capture-MS Homo sapiens
47 DCTN1 1639
Proximity Label-MS Homo sapiens
48 Ptbp1 19205
Affinity Capture-MS Mus musculus
49 HAUS7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
50 HSPB8 26353
Affinity Capture-MS Homo sapiens
51 LRRC29  
Affinity Capture-MS Homo sapiens
52 UBXN6 80700
Affinity Capture-MS Homo sapiens
53 STK32C  
Affinity Capture-MS Homo sapiens
54 NTRK1 4914
Affinity Capture-MS Homo sapiens
55 TUBB 203068
Affinity Capture-MS Homo sapiens
56 WHAMMP3  
Affinity Capture-MS Homo sapiens
57 YAE1D1  
Two-hybrid Homo sapiens
58 ELMO3 79767
Affinity Capture-MS Homo sapiens
59 MIF4GD 57409
Affinity Capture-MS Homo sapiens
60 ZHX1-C8orf76  
Affinity Capture-MS Homo sapiens
61 TMOD4  
Affinity Capture-MS Homo sapiens
62 XPO1 7514
Affinity Capture-MS Homo sapiens
63 BAG2 9532
Affinity Capture-MS Homo sapiens
64 EGFR 1956
Negative Genetic Homo sapiens
65 HOOK3 84376
Proximity Label-MS Homo sapiens
66 TFPT  
Affinity Capture-MS Homo sapiens
67 METTL21B  
Affinity Capture-MS Homo sapiens
68 DUSP16  
Affinity Capture-MS Homo sapiens
69 KRT27 342574
Affinity Capture-MS Homo sapiens
70 CRYBB3  
Affinity Capture-MS Homo sapiens
71 CEP250 11190
Affinity Capture-MS Homo sapiens
72 IFI35 3430
Affinity Capture-MS Homo sapiens
73 RPA3 6119
Proximity Label-MS Homo sapiens
74 SOHLH1  
Affinity Capture-MS Homo sapiens
75 AMZ1  
Affinity Capture-MS Homo sapiens
76 NDC80 10403
Proximity Label-MS Homo sapiens
77 BICD1 636
Proximity Label-MS Homo sapiens
78 SF3B3 23450
Affinity Capture-MS Homo sapiens
79 ILK 3611
Affinity Capture-MS Homo sapiens
80 PCM1 5108
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
81 KRT19 3880
Proximity Label-MS Homo sapiens
82 HAUS3  
Cross-Linking-MS (XL-MS) Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 CCDC8  
Affinity Capture-MS Homo sapiens
84 ZRANB1 54764
Affinity Capture-MS Homo sapiens
85 KRT18 3875
Proximity Label-MS Homo sapiens
86 SPICE1  
Affinity Capture-MS Homo sapiens
87 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
88 KIF14 9928
Affinity Capture-MS Homo sapiens
89 SWSAP1  
Affinity Capture-MS Homo sapiens
90 HAUS1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
91 FBXL16  
Affinity Capture-MS Homo sapiens
92 DCTN2 10540
Proximity Label-MS Homo sapiens
93 LRIF1  
Affinity Capture-MS Homo sapiens
94 FYCO1 79443
Affinity Capture-MS Homo sapiens
95 MAGEA9  
Affinity Capture-MS Homo sapiens
96 FKBP5 2289
Affinity Capture-MS Homo sapiens
97 MKI67  
Affinity Capture-MS Homo sapiens
98 ANAPC2 29882
Proximity Label-MS Homo sapiens
99 C6orf141  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 PPP4R1L  
Affinity Capture-MS Homo sapiens
101 DHFR 1719
Affinity Capture-MS Homo sapiens
102 USP28 57646
Affinity Capture-MS Homo sapiens
103 KRT38 8687
Affinity Capture-MS Homo sapiens
104 INF2 64423
Affinity Capture-MS Homo sapiens
105 PRKAG3  
Affinity Capture-MS Homo sapiens
106 RAB5C 5878
Affinity Capture-MS Homo sapiens
107 INSC  
Affinity Capture-MS Homo sapiens
108 CCDC96  
Affinity Capture-MS Homo sapiens
109 LURAP1  
Affinity Capture-MS Homo sapiens
110 NIN 51199
Proximity Label-MS Homo sapiens
111 C17orf59 54785
Proximity Label-MS Homo sapiens
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