Gene description for CDK5RAP2
Gene name CDK5 regulatory subunit associated protein 2
Gene symbol CDK5RAP2
Other names/aliases C48
Cep215
MCPH3
Species Homo sapiens
 Database cross references - CDK5RAP2
ExoCarta ExoCarta_55755
Entrez Gene 55755
HGNC 18672
MIM 608201
UniProt Q96SN8  
 CDK5RAP2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
 Gene ontology annotations for CDK5RAP2
Molecular Function
    microtubule binding GO:0008017 IDA
    protein binding GO:0005515 IPI
    calmodulin binding GO:0005516 IPI
    protein kinase binding GO:0019901 IPI
    tubulin binding GO:0015631 IPI
    transcription regulatory region DNA binding GO:0044212 IDA
Biological Process
    regulation of spindle checkpoint GO:0090231 IDA
    establishment of mitotic spindle orientation GO:0000132 ISS
    neurogenesis GO:0022008 ISS
    microtubule bundle formation GO:0001578 IDA
    negative regulation of neuron differentiation GO:0045665 IEA
    negative regulation of centriole replication GO:0046600 ISS
    centrosome organization GO:0051297 IMP
    organelle organization GO:0006996 TAS
    mitotic cell cycle GO:0000278 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    microtubule cytoskeleton organization GO:0000226 IDA
    regulation of neuron differentiation GO:0045664 NAS
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    brain development GO:0007420 NAS
    chromosome segregation GO:0007059 IMP
Subcellular Localization
    microtubule GO:0005874 IDA
    centrosome GO:0005813 IDA
    spindle pole GO:0000922 IDA
    cytosol GO:0005829 TAS
    cell junction GO:0030054 IDA
    cytoplasm GO:0005737 IDA
    pericentriolar material GO:0000242 IDA
    Golgi apparatus GO:0005794 IDA
    cytoskeleton GO:0005856 NAS
    extracellular exosome GO:0070062 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
 Experiment description of studies that identified CDK5RAP2 in exosomes
1
Experiment ID 236
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name MHCC97L
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
3
Experiment ID 193
ISEV standards
✔
EM
EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✔
PHB
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 194
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 195
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CDK5RAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DKFZP564O0523  
Two-hybrid Homo sapiens
2 CDK5R1  
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
3 ZNF259  
Two-hybrid Homo sapiens
4 PTN  
Two-hybrid Homo sapiens
5 HAP1  
Two-hybrid Homo sapiens
6 HTATIP  
Two-hybrid Homo sapiens
7 SENP2  
Two-hybrid Homo sapiens
8 C14orf1 11161
Two-hybrid Homo sapiens
9 STARD9 57519
Two-hybrid Homo sapiens
10 EEF1G 1937
Two-hybrid Homo sapiens
11 LUC7L2  
Two-hybrid Homo sapiens
12 MAGEH1  
Two-hybrid Homo sapiens
13 CRMP1 1400
Two-hybrid Homo sapiens
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