Gene description for RBPJ
Gene name recombination signal binding protein for immunoglobulin kappa J region
Gene symbol RBPJ
Other names/aliases AOS3
CBF1
IGKJRB
IGKJRB1
KBF2
RBP-J
RBPJK
RBPSUH
SUH
csl
Species Homo sapiens
 Database cross references - RBPJ
ExoCarta ExoCarta_3516
Vesiclepedia VP_3516
Entrez Gene 3516
HGNC 5724
MIM 147183
UniProt Q06330  
 RBPJ identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for RBPJ
Molecular Function
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IDA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription activator activity, RNA polymerase II-specific GO:0001228 IDA
    DNA binding GO:0003677 TAS
    chromatin binding GO:0003682 IEA
    DNA-binding transcription factor activity GO:0003700 TAS
    protein binding GO:0005515 IPI
    sequence-specific DNA binding GO:0043565 IDA
    RNA polymerase II-specific DNA-binding transcription factor binding GO:0061629 IPI
    DNA-binding transcription factor binding GO:0140297 IDA
    DNA-binding transcription factor binding GO:0140297 IPI
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    negative regulation of transcription by RNA polymerase II GO:0000122 ISS
    angiogenesis GO:0001525 ISS
    somitogenesis GO:0001756 IEA
    epithelial to mesenchymal transition GO:0001837 ISS
    blood vessel remodeling GO:0001974 ISS
    inflammatory response to antigenic stimulus GO:0002437 IEA
    secondary heart field specification GO:0003139 IEA
    outflow tract morphogenesis GO:0003151 ISS
    endocardium morphogenesis GO:0003160 ISS
    aortic valve development GO:0003176 ISS
    pulmonary valve development GO:0003177 ISS
    epithelial to mesenchymal transition involved in endocardial cushion formation GO:0003198 ISS
    cardiac left ventricle morphogenesis GO:0003214 ISS
    ventricular trabecula myocardium morphogenesis GO:0003222 ISS
    regulation of transcription by RNA polymerase II GO:0006357 ISS
    humoral immune response GO:0006959 IEA
    Notch signaling pathway GO:0007219 IBA
    Notch signaling pathway GO:0007219 IMP
    positive regulation of transcription of Notch receptor target GO:0007221 IDA
    heart development GO:0007507 IMP
    auditory receptor cell fate commitment GO:0009912 IEA
    epidermal cell fate specification GO:0009957 IEA
    positive regulation of gene expression GO:0010628 IEA
    pituitary gland development GO:0021983 IEA
    B cell differentiation GO:0030183 IEA
    keratinocyte differentiation GO:0030216 IEA
    negative regulation of ossification GO:0030279 ISS
    positive regulation of BMP signaling pathway GO:0030513 ISS
    somatic stem cell population maintenance GO:0035019 IEA
    dorsal aorta morphogenesis GO:0035912 ISS
    atrioventricular canal development GO:0036302 ISS
    defense response to bacterium GO:0042742 IEA
    myeloid dendritic cell differentiation GO:0043011 IEA
    regulation of generation of precursor metabolites and energy GO:0043467 IDA
    negative regulation of cell differentiation GO:0045596 IEA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IDA
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 ISS
    regulation of timing of cell differentiation GO:0048505 IEA
    sebaceous gland development GO:0048733 IEA
    hair follicle maturation GO:0048820 IEA
    epithelial cell proliferation GO:0050673 IEA
    positive regulation of epithelial cell proliferation GO:0050679 IEA
    positive regulation of cardiac muscle cell proliferation GO:0060045 ISS
    cardiac muscle cell myoblast differentiation GO:0060379 ISS
    ventricular septum morphogenesis GO:0060412 IEA
    club cell differentiation GO:0060486 IEA
    labyrinthine layer blood vessel development GO:0060716 ISS
    arterial endothelial cell fate commitment GO:0060844 IEA
    regulation of cell adhesion involved in heart morphogenesis GO:0061344 IEA
    stem cell proliferation GO:0072089 IEA
    blood vessel lumenization GO:0072554 ISS
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    blood vessel endothelial cell fate specification GO:0097101 ISS
    negative regulation of cold-induced thermogenesis GO:0120163 ISS
    positive regulation of ERBB signaling pathway GO:1901186 ISS
    positive regulation of ephrin receptor signaling pathway GO:1901189 ISS
    positive regulation of cell proliferation involved in heart morphogenesis GO:2000138 ISS
    negative regulation of stem cell proliferation GO:2000647 IEA
Subcellular Localization
    chromatin GO:0000785 ISA
    MAML1-RBP-Jkappa- ICN1 complex GO:0002193 IBA
    MAML1-RBP-Jkappa- ICN1 complex GO:0002193 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
    nucleoplasm GO:0005654 TAS
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    transcription repressor complex GO:0017053 IDA
 Experiment description of studies that identified RBPJ in sEVs
1
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for RBPJ
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 HNRNPH1 3187
Affinity Capture-RNA Homo sapiens
2 NACC1 112939
Affinity Capture-MS Homo sapiens
3 NOTCH3 4854
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 CAMSAP1 157922
Affinity Capture-MS Homo sapiens
5 HDAC2 3066
Affinity Capture-MS Homo sapiens
6 PITX2  
Affinity Capture-MS Homo sapiens
7 JMJD1C 221037
Affinity Capture-MS Homo sapiens
8 GADD45A  
Two-hybrid Homo sapiens
9 SP1  
Co-fractionation Homo sapiens
10 CGGBP1 8545
Affinity Capture-MS Homo sapiens
11 PTF1A  
Two-hybrid Homo sapiens
12 SAMD1  
Affinity Capture-MS Homo sapiens
13 CREM  
Affinity Capture-MS Homo sapiens
14 ARID3B  
Affinity Capture-MS Homo sapiens
15 FBXO42  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 SATB2  
Affinity Capture-MS Homo sapiens
17 PSIP1 11168
Affinity Capture-MS Homo sapiens
18 HOXB9  
Affinity Capture-MS Homo sapiens
19 ZMIZ1 57178
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
20 ZBTB10 65986
Affinity Capture-MS Homo sapiens
21 DDX24 57062
Affinity Capture-MS Homo sapiens
22 SMARCA1 6594
Affinity Capture-MS Homo sapiens
23 KDM2A  
Affinity Capture-MS Homo sapiens
24 NR2F2  
Affinity Capture-MS Homo sapiens
25 TFAP4 7023
Affinity Capture-MS Homo sapiens
26 CUX1 1523
Affinity Capture-MS Homo sapiens
27 MBD1  
Affinity Capture-MS Homo sapiens
28 MED27  
Two-hybrid Homo sapiens
29 Notch1  
Affinity Capture-Western Mus musculus
30 GTF3C5 9328
Affinity Capture-MS Homo sapiens
31 RCC1 1104
Affinity Capture-MS Homo sapiens
32 CDC27 996
Affinity Capture-MS Homo sapiens
33 ALX4  
Affinity Capture-MS Homo sapiens
34 HMG20B  
Affinity Capture-MS Homo sapiens
35 POLR2C 5432
Proximity Label-MS Homo sapiens
36 MECP2 4204
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
37 CUL1 8454
Affinity Capture-MS Homo sapiens
38 BCL6B  
Affinity Capture-Western Homo sapiens
39 GSK3B 2932
Two-hybrid Homo sapiens
40 NOTCH2 4853
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 DLX5  
Affinity Capture-MS Homo sapiens
42 LCOR 84458
Affinity Capture-MS Homo sapiens
43 POP1 10940
Affinity Capture-MS Homo sapiens
44 ZNF268  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
46 WDR70 55100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 PARK2  
Affinity Capture-MS Homo sapiens
48 NKX6-1 4825
Affinity Capture-MS Homo sapiens
49 TARBP2  
Affinity Capture-Western Homo sapiens
50 NCOR2  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
51 RECQL 5965
Affinity Capture-MS Homo sapiens
52 NFIX 4784
Affinity Capture-MS Homo sapiens
53 CFDP1  
Affinity Capture-MS Homo sapiens
54 NSMF  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
55 SMARCA4 6597
Affinity Capture-Western Homo sapiens
56 MKRN2 23609
Affinity Capture-RNA Homo sapiens
57 MMAB 326625
Affinity Capture-MS Homo sapiens
58 FANCG 2189
Affinity Capture-Western Homo sapiens
59 RPA2 6118
Affinity Capture-MS Homo sapiens
60 HOXC13  
Affinity Capture-MS Homo sapiens
61 OGT 8473
Affinity Capture-MS Homo sapiens
62 DEK 7913
Affinity Capture-MS Homo sapiens
63 NFATC1 4772
Affinity Capture-MS Homo sapiens
64 NFIA 4774
Affinity Capture-MS Homo sapiens
65 MBD2 8932
Affinity Capture-MS Homo sapiens
66 FNBP1 23048
Affinity Capture-MS Homo sapiens
67 lid  
Reconstituted Complex Drosophila melanogaster
68 SND1 27044
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
69 H2AFY 9555
Affinity Capture-MS Homo sapiens
70 KDM5A  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-purification Homo sapiens
71 KIF2C 11004
Affinity Capture-MS Homo sapiens
72 RPS6KA6 27330
Two-hybrid Homo sapiens
73 SOX7  
Proximity Label-MS Homo sapiens
74 TPX2  
Affinity Capture-MS Homo sapiens
75 GATAD2A 54815
Affinity Capture-MS Homo sapiens
76 CD40 958
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
77 PATZ1  
Affinity Capture-MS Homo sapiens
78 FNBP1L 54874
Affinity Capture-MS Homo sapiens
79 HOXA10  
Affinity Capture-MS Homo sapiens
80 NKX2-5  
Affinity Capture-MS Homo sapiens
81 XPO1 7514
Affinity Capture-MS Homo sapiens
82 SMARCA5 8467
Affinity Capture-MS Homo sapiens
83 MAML1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
84 CD6 923
Affinity Capture-MS Homo sapiens
85 ZNF143  
Affinity Capture-MS Homo sapiens
86 CUL3 8452
Affinity Capture-MS Homo sapiens
87 ADNP 23394
Affinity Capture-MS Homo sapiens
88 DCAF16 54876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
89 GTF3C4 9329
Affinity Capture-MS Homo sapiens
90 RUNX3  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
91 TBP  
Affinity Capture-MS Homo sapiens
92 HNF4A  
Proximity Label-MS Homo sapiens
93 FANCA  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
94 RND3 390
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
95 CAMK4 814
Affinity Capture-Western Homo sapiens
96 SPEN 23013
Co-fractionation Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 RPA3 6119
Proximity Label-MS Homo sapiens
98 USP7 7874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
99 PHF21A  
Affinity Capture-MS Homo sapiens
100 KAT5  
Affinity Capture-Western Homo sapiens
101 SART3 9733
Affinity Capture-MS Homo sapiens
102 MYH7 4625
Affinity Capture-MS Homo sapiens
103 BEND3  
Affinity Capture-MS Homo sapiens
104 TXNDC5 81567
Affinity Capture-MS Homo sapiens
105 CCND1 595
Co-fractionation Homo sapiens
106 ADAR 103
Affinity Capture-MS Homo sapiens
107 ZNF589  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
108 SFMBT1 51460
Affinity Capture-MS Homo sapiens
109 SATB1 6304
Affinity Capture-MS Homo sapiens
110 APP 351
Reconstituted Complex Homo sapiens
111 EIF1AD 84285
Affinity Capture-MS Homo sapiens
112 CIR1  
Two-hybrid Homo sapiens
113 FHL1 2273
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
114 SPOP  
Two-hybrid Homo sapiens
115 KAT2A  
Two-hybrid Homo sapiens
116 SIN3A  
Reconstituted Complex Homo sapiens
117 HOXD13  
Affinity Capture-MS Homo sapiens
118 KAT2B  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
119 CLPTM1 1209
Affinity Capture-MS Homo sapiens
120 H  
Reconstituted Complex Drosophila melanogaster
121 HSPB1 3315
Two-hybrid Homo sapiens
122 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
123 SEPSECS  
Affinity Capture-MS Homo sapiens
124 RITA1  
Affinity Capture-MS Homo sapiens
125 CHD4 1108
Affinity Capture-MS Homo sapiens
126 RNF20 56254
Affinity Capture-MS Homo sapiens
127 RUNX1T1  
Affinity Capture-Western Homo sapiens
128 MAP7D3 79649
Affinity Capture-MS Homo sapiens
129 RNF7  
Affinity Capture-MS Homo sapiens
130 TPM2 7169
Affinity Capture-MS Homo sapiens
131 PBX1 5087
Affinity Capture-MS Homo sapiens
132 TPM1 7168
Affinity Capture-MS Homo sapiens
133 KDM1A 23028
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
134 L3MBTL3  
Affinity Capture-MS Homo sapiens
135 HES1 3280
Co-crystal Structure Homo sapiens
136 SNW1 22938
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
137 CAMK2D 817
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 NFIC 4782
Affinity Capture-MS Homo sapiens
139 AR 367
Proximity Label-MS Homo sapiens
140 MYC  
Affinity Capture-MS Homo sapiens
141 CHAF1B  
Affinity Capture-MS Homo sapiens
142 CUEDC1 404093
Affinity Capture-MS Homo sapiens
143 PHF8  
Affinity Capture-Western Homo sapiens
144 USP47 55031
Affinity Capture-MS Homo sapiens
145 NOTCH1 4851
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
146 RANGAP1 5905
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which RBPJ is involved
PathwayEvidenceSource
Constitutive Signaling by NOTCH1 HD+PEST Domain Mutants TAS Reactome
Constitutive Signaling by NOTCH1 PEST Domain Mutants TAS Reactome
Developmental Biology IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Formation of paraxial mesoderm IEA Reactome
Gastrulation IEA Reactome
Gene expression (Transcription) IEA Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway IEA Reactome
Generic Transcription Pathway TAS Reactome
Notch-HLH transcription pathway TAS Reactome
NOTCH1 Intracellular Domain Regulates Transcription TAS Reactome
NOTCH1 Intracellular Domain Regulates Transcription IEA Reactome
NOTCH2 intracellular domain regulates transcription IEA Reactome
NOTCH2 intracellular domain regulates transcription TAS Reactome
NOTCH3 Intracellular Domain Regulates Transcription TAS Reactome
NOTCH3 Intracellular Domain Regulates Transcription IEA Reactome
NOTCH4 Intracellular Domain Regulates Transcription TAS Reactome
NOTCH4 Intracellular Domain Regulates Transcription IEA Reactome
Pre-NOTCH Expression and Processing IEA Reactome
Pre-NOTCH Expression and Processing TAS Reactome
Pre-NOTCH Transcription and Translation IEA Reactome
Pre-NOTCH Transcription and Translation TAS Reactome
Regulation of beta-cell development IEA Reactome
Regulation of gene expression in late stage (branching morphogenesis) pancreatic bud precursor cells IEA Reactome
RNA Polymerase II Transcription IEA Reactome
RNA Polymerase II Transcription TAS Reactome
RUNX3 regulates NOTCH signaling IEA Reactome
RUNX3 regulates NOTCH signaling TAS Reactome
Signal Transduction IEA Reactome
Signal Transduction TAS Reactome
Signaling by NOTCH IEA Reactome
Signaling by NOTCH TAS Reactome
Signaling by NOTCH1 TAS Reactome
Signaling by NOTCH1 IEA Reactome
Signaling by NOTCH1 HD+PEST Domain Mutants in Cancer TAS Reactome
Signaling by NOTCH1 in Cancer TAS Reactome
Signaling by NOTCH1 PEST Domain Mutants in Cancer TAS Reactome
Signaling by NOTCH2 IEA Reactome
Signaling by NOTCH2 TAS Reactome
Signaling by NOTCH3 TAS Reactome
Signaling by NOTCH3 IEA Reactome
Signaling by NOTCH4 TAS Reactome
Signaling by NOTCH4 IEA Reactome
Somitogenesis IEA Reactome
Transcriptional regulation by RUNX3 IEA Reactome
Transcriptional regulation by RUNX3 TAS Reactome





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