Gene description for EGLN1
Gene name egl-9 family hypoxia-inducible factor 1
Gene symbol EGLN1
Other names/aliases C1orf12
ECYT3
HALAH
HIF-PH2
HIFPH2
HPH-2
HPH2
PHD2
SM20
ZMYND6
Species Homo sapiens
 Database cross references - EGLN1
ExoCarta ExoCarta_54583
Vesiclepedia VP_54583
Entrez Gene 54583
HGNC 1232
MIM 606425
UniProt Q9GZT9  
 EGLN1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for EGLN1
Molecular Function
    protein binding GO:0005515 IPI
    ferrous iron binding GO:0008198 IBA
    ferrous iron binding GO:0008198 IDA
    2-oxoglutarate-dependent dioxygenase activity GO:0016706 IDA
    enzyme binding GO:0019899 ISS
    L-ascorbic acid binding GO:0031418 IEA
    peptidyl-proline dioxygenase activity GO:0031543 TAS
    peptidyl-proline 4-dioxygenase activity GO:0031545 IBA
    hypoxia-inducible factor-proline dioxygenase activity GO:0160082 IDA
Biological Process
    response to hypoxia GO:0001666 IDA
    intracellular iron ion homeostasis GO:0006879 IEA
    intracellular oxygen homeostasis GO:0032364 IDA
    negative regulation of DNA-binding transcription factor activity GO:0043433 IDA
    regulation of neuron apoptotic process GO:0043523 IBA
    regulation of angiogenesis GO:0045765 ISS
    positive regulation of transcription by RNA polymerase II GO:0045944 IEA
    negative regulation of cyclic-nucleotide phosphodiesterase activity GO:0051344 ISS
    cardiac muscle tissue morphogenesis GO:0055008 IEA
    heart trabecula formation GO:0060347 IEA
    ventricular septum morphogenesis GO:0060412 IEA
    labyrinthine layer development GO:0060711 IEA
    cellular response to hypoxia GO:0071456 IBA
    response to nitric oxide GO:0071731 IDA
    regulation of modification of postsynaptic structure GO:0099159 IEA
    regulation protein catabolic process at postsynapse GO:0140252 IEA
Subcellular Localization
    nucleus GO:0005634 IBA
    cytoplasm GO:0005737 IBA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    postsynaptic density GO:0014069 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified EGLN1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for EGLN1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 POLR2C 5432
Proximity Label-MS Homo sapiens
2 MAGEA11  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 SIAH2 6478
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
4 LCK 3932
Proximity Label-MS Homo sapiens
5 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
6 CITED1 4435
Affinity Capture-MS Homo sapiens
7 HIST1H3A 8350
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
8 RHOBTB3 22836
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 MAGEA9  
Affinity Capture-Western Homo sapiens
10 EGLN3  
Affinity Capture-Western Homo sapiens
11 PKM 5315
Affinity Capture-Western Homo sapiens
12 PEX19 5824
Affinity Capture-MS Homo sapiens
13 EPAS1  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
14 BTF3 689
Affinity Capture-MS Homo sapiens
15 OXSR1 9943
Co-fractionation Homo sapiens
16 ING4  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
17 CUL3 8452
Affinity Capture-MS Homo sapiens
18 SNUPN 10073
Affinity Capture-MS Homo sapiens
19 CBWD2  
Affinity Capture-MS Homo sapiens
20 HSP90AB1 3326
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 EGLN1 54583
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
22 VHL  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
23 FKBP8 23770
Reconstituted Complex Homo sapiens
FRET Homo sapiens
FRET Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
24 TET2  
Affinity Capture-Western Homo sapiens
25 PPP1R13L 10848
Affinity Capture-Western Homo sapiens
26 OS9 10956
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 FKBP5 2289
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
28 POLR2A 5430
Affinity Capture-Western Homo sapiens
29 PLD1 5337
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
30 APOBEC3C 27350
Affinity Capture-MS Homo sapiens
31 TET3  
Affinity Capture-Western Homo sapiens
32 RFPL4B  
Affinity Capture-MS Homo sapiens
33 AKAP1 8165
Proximity Label-MS Homo sapiens
34 FKBP4 2288
Affinity Capture-MS Homo sapiens
35 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
36 BPNT1 10380
Co-fractionation Homo sapiens
37 AKT2 208
Affinity Capture-Western Homo sapiens
38 LIMD1 8994
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
39 ZNF281  
Two-hybrid Homo sapiens
40 PTGES3 10728
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
41 SRC 6714
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
42 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
PCA Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
43 KRAS 3845
Two-hybrid Homo sapiens
44 AKT1 207
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
45 GPLD1  
Affinity Capture-Western Homo sapiens
46 N6AMT2 221143
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
47 CBWD1 55871
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
48 PLD2 5338
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
49 OSR1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
50 SPRY2 10253
Affinity Capture-Western Homo sapiens
51 RMDN3 55177
Proximity Label-MS Homo sapiens
52 LMP2  
Reconstituted Complex
53 HIF1A 3091
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
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